Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome
Metabolic syndrome (MetS) is a chronic disease associated with the disturbance of gut microbiota homeostasis. Metabolites derived from gut microbes play essential roles in MetS prevention and therapy. Here, we focused on the inhibitory effect of the extract of millet bran protein (EMBP) on a high-fa...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250165 |
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| author | Shuhua Shan Ruopeng Yin Jiangying Shi Lizhen Zhang Jiaqi Zhou Qinqin Qiao Xiushan Dong Wenjing Zhao Zhuoyu Li |
| author_facet | Shuhua Shan Ruopeng Yin Jiangying Shi Lizhen Zhang Jiaqi Zhou Qinqin Qiao Xiushan Dong Wenjing Zhao Zhuoyu Li |
| author_sort | Shuhua Shan |
| collection | DOAJ |
| description | Metabolic syndrome (MetS) is a chronic disease associated with the disturbance of gut microbiota homeostasis. Metabolites derived from gut microbes play essential roles in MetS prevention and therapy. Here, we focused on the inhibitory effect of the extract of millet bran protein (EMBP) on a high-fat diet (HFD)-induced MetS, aiming to identify gut microbiota and their metabolites that involve in the anti-MetS activity of EMBP. The obesity, chronic inflammation, insulin resistance in MetS mouse models were abolished after EMBP treatment. The protective mechanism of EMBP against HFD-induced MetS may depend on improved gut barrier function. Using microbiome analysis, we found that EMBP supplementation improved gut microbiome dysbiosis in MetS mice, specifically upregulating Bacteroides acidifaciens. The fecal microbiota transplantation (FMT) also demonstrated this phenomenon. In addition, metabolomic analysis showed that EMBP mediates metabolic profiling reprogramming in MetS mice. Notably, a microbiota-derived metabolite, gamma-aminobutyric acid (GABA), is enriched by EMBP. In addition, exogenous GABA treatment produced a similar protective effect to EMBP by improving NRF2-dependent gut barrier function to protect HFD-induced MetS. The results suggest that EMBP suppress host MetS by remodeling of gut microbiota as an effective candidate for next-generation medicine food dual purpose dietary supplement to intervene in MetS. |
| format | Article |
| id | doaj-art-96310b733db54aac92de3c8647235b6d |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-96310b733db54aac92de3c8647235b6d2025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011341987200110.26599/FSHW.2022.9250165Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndromeShuhua Shan0Ruopeng Yin1Jiangying Shi2Lizhen Zhang3Jiaqi Zhou4Qinqin Qiao5Xiushan Dong6Wenjing Zhao7Zhuoyu Li8Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaInstitute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, ChinaInstitute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, ChinaDepartment of Biology, Biological Science and Technology College, Taiyuan Normal University, Jinzhong 030619, ChinaInstitute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, ChinaMetabolic syndrome (MetS) is a chronic disease associated with the disturbance of gut microbiota homeostasis. Metabolites derived from gut microbes play essential roles in MetS prevention and therapy. Here, we focused on the inhibitory effect of the extract of millet bran protein (EMBP) on a high-fat diet (HFD)-induced MetS, aiming to identify gut microbiota and their metabolites that involve in the anti-MetS activity of EMBP. The obesity, chronic inflammation, insulin resistance in MetS mouse models were abolished after EMBP treatment. The protective mechanism of EMBP against HFD-induced MetS may depend on improved gut barrier function. Using microbiome analysis, we found that EMBP supplementation improved gut microbiome dysbiosis in MetS mice, specifically upregulating Bacteroides acidifaciens. The fecal microbiota transplantation (FMT) also demonstrated this phenomenon. In addition, metabolomic analysis showed that EMBP mediates metabolic profiling reprogramming in MetS mice. Notably, a microbiota-derived metabolite, gamma-aminobutyric acid (GABA), is enriched by EMBP. In addition, exogenous GABA treatment produced a similar protective effect to EMBP by improving NRF2-dependent gut barrier function to protect HFD-induced MetS. The results suggest that EMBP suppress host MetS by remodeling of gut microbiota as an effective candidate for next-generation medicine food dual purpose dietary supplement to intervene in MetS.https://www.sciopen.com/article/10.26599/FSHW.2022.9250165metabolic syndromegut microbiotaextract of millet bran proteingamma-aminobutyric acidgut barrier function |
| spellingShingle | Shuhua Shan Ruopeng Yin Jiangying Shi Lizhen Zhang Jiaqi Zhou Qinqin Qiao Xiushan Dong Wenjing Zhao Zhuoyu Li Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome Food Science and Human Wellness metabolic syndrome gut microbiota extract of millet bran protein gamma-aminobutyric acid gut barrier function |
| title | Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome |
| title_full | Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome |
| title_fullStr | Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome |
| title_full_unstemmed | Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome |
| title_short | Gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome |
| title_sort | gut microbiota remodeling drived by dietary millet protein prevents the metabolic syndrome |
| topic | metabolic syndrome gut microbiota extract of millet bran protein gamma-aminobutyric acid gut barrier function |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250165 |
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