Identification and characterization of the GH3 gene family in maize
Abstract: The phytohormone auxin plays a central role in coordinating plant growth and development. GH3 is one of the three gene families that respond rapidly during auxin stimulation. Here, we report the identification and characterization of the GH3 gene family in maize. A total of 12 GH3 genes we...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2016-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915610760 |
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| author | Dong-feng ZHANG Nan ZHANG Tao ZHONG Chao WANG Ming-liang XU Jian-rong YE |
| author_facet | Dong-feng ZHANG Nan ZHANG Tao ZHONG Chao WANG Ming-liang XU Jian-rong YE |
| author_sort | Dong-feng ZHANG |
| collection | DOAJ |
| description | Abstract: The phytohormone auxin plays a central role in coordinating plant growth and development. GH3 is one of the three gene families that respond rapidly during auxin stimulation. Here, we report the identification and characterization of the GH3 gene family in maize. A total of 12 GH3 genes were identified, which are not evenly distributed over the 10 maize chromosomes. Maize GH3 protein sequences share a conserved domain which occupies nearly the entire protein. Diversified cis-elements were found in promoters of maize GH3 genes. In this study, the 12 maize GH3 proteins were primarily classified into two phylogenetic groups, similar to the 13 rice GH3 proteins, while 9 of the 19 Arabidopsis GH3 proteins were observed in the third phylogenetic group. Microarray analysis showed that expression of maize GH3 genes is temporally and spatially modulated. Additionally, maize GH3 genes displayed variable changes at transcript level upon pathogen infection. Results presented here provide insight into the diversification and evolution of GH3 proteins, and lay a foundation for the functional characterization of these GH3 genes in future, especially for elucidating the mechanisms of GH3-mediated pathogenesis. |
| format | Article |
| id | doaj-art-961fcb7ec1fd420da12e51c4a070402d |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-961fcb7ec1fd420da12e51c4a070402d2025-08-20T03:57:04ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-02-0115224926110.1016/S2095-3119(15)61076-0Identification and characterization of the GH3 gene family in maizeDong-feng ZHANG0Nan ZHANG1Tao ZHONG2Chao WANG3Ming-liang XU4Jian-rong YE5National Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaNational Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaNational Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaNational Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaNational Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaCorrespondence YE Jian-rong, Tel: +86-10-62731135, Fax: +86-10-62733808; National Maize Improvement Center/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.ChinaAbstract: The phytohormone auxin plays a central role in coordinating plant growth and development. GH3 is one of the three gene families that respond rapidly during auxin stimulation. Here, we report the identification and characterization of the GH3 gene family in maize. A total of 12 GH3 genes were identified, which are not evenly distributed over the 10 maize chromosomes. Maize GH3 protein sequences share a conserved domain which occupies nearly the entire protein. Diversified cis-elements were found in promoters of maize GH3 genes. In this study, the 12 maize GH3 proteins were primarily classified into two phylogenetic groups, similar to the 13 rice GH3 proteins, while 9 of the 19 Arabidopsis GH3 proteins were observed in the third phylogenetic group. Microarray analysis showed that expression of maize GH3 genes is temporally and spatially modulated. Additionally, maize GH3 genes displayed variable changes at transcript level upon pathogen infection. Results presented here provide insight into the diversification and evolution of GH3 proteins, and lay a foundation for the functional characterization of these GH3 genes in future, especially for elucidating the mechanisms of GH3-mediated pathogenesis.http://www.sciencedirect.com/science/article/pii/S2095311915610760auxinGH3 familypathogen infectionmaize (Zea mays L.) |
| spellingShingle | Dong-feng ZHANG Nan ZHANG Tao ZHONG Chao WANG Ming-liang XU Jian-rong YE Identification and characterization of the GH3 gene family in maize Journal of Integrative Agriculture auxin GH3 family pathogen infection maize (Zea mays L.) |
| title | Identification and characterization of the GH3 gene family in maize |
| title_full | Identification and characterization of the GH3 gene family in maize |
| title_fullStr | Identification and characterization of the GH3 gene family in maize |
| title_full_unstemmed | Identification and characterization of the GH3 gene family in maize |
| title_short | Identification and characterization of the GH3 gene family in maize |
| title_sort | identification and characterization of the gh3 gene family in maize |
| topic | auxin GH3 family pathogen infection maize (Zea mays L.) |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915610760 |
| work_keys_str_mv | AT dongfengzhang identificationandcharacterizationofthegh3genefamilyinmaize AT nanzhang identificationandcharacterizationofthegh3genefamilyinmaize AT taozhong identificationandcharacterizationofthegh3genefamilyinmaize AT chaowang identificationandcharacterizationofthegh3genefamilyinmaize AT mingliangxu identificationandcharacterizationofthegh3genefamilyinmaize AT jianrongye identificationandcharacterizationofthegh3genefamilyinmaize |