Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.
RNA silencing, mediated by small RNAs including microRNAs (miRNAs) and small interfering RNAs (siRNAs), is a potent antiviral or antibacterial mechanism, besides regulating normal cellular gene expression critical for development and physiology. To gain insights into host small RNA metabolism under...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-08-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002176&type=printable |
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| author | Peng Du Jianguo Wu Jiayao Zhang Shuqi Zhao Hong Zheng Ge Gao Liping Wei Yi Li |
| author_facet | Peng Du Jianguo Wu Jiayao Zhang Shuqi Zhao Hong Zheng Ge Gao Liping Wei Yi Li |
| author_sort | Peng Du |
| collection | DOAJ |
| description | RNA silencing, mediated by small RNAs including microRNAs (miRNAs) and small interfering RNAs (siRNAs), is a potent antiviral or antibacterial mechanism, besides regulating normal cellular gene expression critical for development and physiology. To gain insights into host small RNA metabolism under infections by different viruses, we used Solexa/Illumina deep sequencing to characterize the small RNA profiles of rice plants infected by two distinct viruses, Rice dwarf virus (RDV, dsRNA virus) and Rice stripe virus (RSV, a negative sense and ambisense RNA virus), respectively, as compared with those from non-infected plants. Our analyses showed that RSV infection enhanced the accumulation of some rice miRNA*s, but not their corresponding miRNAs, as well as accumulation of phased siRNAs from a particular precursor. Furthermore, RSV infection also induced the expression of novel miRNAs in a phased pattern from several conserved miRNA precursors. In comparison, no such changes in host small RNA expression was observed in RDV-infected rice plants. Significantly RSV infection elevated the expression levels of selective OsDCLs and OsAGOs, whereas RDV infection only affected the expression of certain OsRDRs. Our results provide a comparative analysis, via deep sequencing, of changes in the small RNA profiles and in the genes of RNA silencing machinery induced by different viruses in a natural and economically important crop host plant. They uncover new mechanisms and complexity of virus-host interactions that may have important implications for further studies on the evolution of cellular small RNA biogenesis that impact pathogen infection, pathogenesis, as well as organismal development. |
| format | Article |
| id | doaj-art-95ea7f832f664360b7d18a683b55e0e4 |
| institution | Kabale University |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2011-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-95ea7f832f664360b7d18a683b55e0e42025-01-16T05:31:02ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742011-08-0178e100217610.1371/journal.ppat.1002176Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.Peng DuJianguo WuJiayao ZhangShuqi ZhaoHong ZhengGe GaoLiping WeiYi LiRNA silencing, mediated by small RNAs including microRNAs (miRNAs) and small interfering RNAs (siRNAs), is a potent antiviral or antibacterial mechanism, besides regulating normal cellular gene expression critical for development and physiology. To gain insights into host small RNA metabolism under infections by different viruses, we used Solexa/Illumina deep sequencing to characterize the small RNA profiles of rice plants infected by two distinct viruses, Rice dwarf virus (RDV, dsRNA virus) and Rice stripe virus (RSV, a negative sense and ambisense RNA virus), respectively, as compared with those from non-infected plants. Our analyses showed that RSV infection enhanced the accumulation of some rice miRNA*s, but not their corresponding miRNAs, as well as accumulation of phased siRNAs from a particular precursor. Furthermore, RSV infection also induced the expression of novel miRNAs in a phased pattern from several conserved miRNA precursors. In comparison, no such changes in host small RNA expression was observed in RDV-infected rice plants. Significantly RSV infection elevated the expression levels of selective OsDCLs and OsAGOs, whereas RDV infection only affected the expression of certain OsRDRs. Our results provide a comparative analysis, via deep sequencing, of changes in the small RNA profiles and in the genes of RNA silencing machinery induced by different viruses in a natural and economically important crop host plant. They uncover new mechanisms and complexity of virus-host interactions that may have important implications for further studies on the evolution of cellular small RNA biogenesis that impact pathogen infection, pathogenesis, as well as organismal development.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002176&type=printable |
| spellingShingle | Peng Du Jianguo Wu Jiayao Zhang Shuqi Zhao Hong Zheng Ge Gao Liping Wei Yi Li Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors. PLoS Pathogens |
| title | Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors. |
| title_full | Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors. |
| title_fullStr | Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors. |
| title_full_unstemmed | Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors. |
| title_short | Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors. |
| title_sort | viral infection induces expression of novel phased micrornas from conserved cellular microrna precursors |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002176&type=printable |
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