Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice
Objective:To study the effect of total saponins of panax notoginseng (PNS) on high-fat diet induced lipids deposition on the aortic tissue of apolipoprotein E-knockout (ApoE<sup>-/-</sup>) mice.Methods:The atherosclerosis models were prepared with a high-fat diet in the ApoE<sup>-/...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Office of Rehabilitation Medicine
2018-06-01
|
| Series: | 康复学报 |
| Subjects: | |
| Online Access: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2018.03027 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841536991904137216 |
|---|---|
| author | Changgeng FU Ruina BAI Yue LIU Linzi LONG Keji CHEN |
| author_facet | Changgeng FU Ruina BAI Yue LIU Linzi LONG Keji CHEN |
| author_sort | Changgeng FU |
| collection | DOAJ |
| description | Objective:To study the effect of total saponins of panax notoginseng (PNS) on high-fat diet induced lipids deposition on the aortic tissue of apolipoprotein E-knockout (ApoE<sup>-/-</sup>) mice.Methods:The atherosclerosis models were prepared with a high-fat diet in the ApoE<sup>-/-</sup>mouse model for 12 weeks. All ApoE<sup>-/-</sup>mice were randomly divided into three groups (model group, simvastatin group and total saponins group),and the wild type C57BL/6J mice were taked as the control group. ApoE<sup>-/-</sup>mice continuely intook high fat feeding by gavage for 8 weeks. Blood and the aortic root tissue were collected and serum lipid level was detected by biochemical analyzer, the serum IL-1β,IL-10 concentration was detected by ELISA, the lipid deposition of aortic tissue was observed by oil red O staining, CD<sub>68</sub><sup>+</sup>macrophage aggregation of aortic tissue was observed by immunohistochemical staining.Results:In the model group, TC and LDL-C were significantly increased, HDL-C was slightly reduced, and IL-1β,IL-10 were also increased significantly (<italic>P</italic><0.05).Aortic tissue oil red O positive staining cells increased significantly (<italic>P</italic><0.05),plaque lipid deposition was evident. The dots or patches protrude into the vascular and the vascular cavity was markedly narrowed, CD<sub>68</sub><sup>+</sup>macrophage was also increased significantly in aortic tissue (all <italic>P</italic><0.05).Compared to the model group, TC, LDL-C and IL-1β,IL-10 were significantly reduced (<italic>P</italic><0.05),HDL-C was slightly increased in both the simvastatin group and the total saponins group (<italic>P</italic><0.05).Positive staining cells of oil red O in aortic tissue, were significantly reduced, plaque lipid deposition and the area decreased. The degree of convexity was significantly reduced. CD<sub>68</sub><sup>+</sup>macrophage number and the macrophage aggregation were significantly reduced (all <italic>P</italic><0.05).Conclusion:PNS can regulate blood lipids, inhibit the immune inflammatory response, inhibit the lipid deposition of aortainhibiting and macrophage aggregation, which might be one of the anti-atherosclerosis mechanisms. |
| format | Article |
| id | doaj-art-9553b9746ade4b6eb9ab1ce8953753b7 |
| institution | Kabale University |
| issn | 2096-0328 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | Editorial Office of Rehabilitation Medicine |
| record_format | Article |
| series | 康复学报 |
| spelling | doaj-art-9553b9746ade4b6eb9ab1ce8953753b72025-01-14T10:05:04ZengEditorial Office of Rehabilitation Medicine康复学报2096-03282018-06-0128273123127337Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout MiceChanggeng FURuina BAIYue LIULinzi LONGKeji CHENObjective:To study the effect of total saponins of panax notoginseng (PNS) on high-fat diet induced lipids deposition on the aortic tissue of apolipoprotein E-knockout (ApoE<sup>-/-</sup>) mice.Methods:The atherosclerosis models were prepared with a high-fat diet in the ApoE<sup>-/-</sup>mouse model for 12 weeks. All ApoE<sup>-/-</sup>mice were randomly divided into three groups (model group, simvastatin group and total saponins group),and the wild type C57BL/6J mice were taked as the control group. ApoE<sup>-/-</sup>mice continuely intook high fat feeding by gavage for 8 weeks. Blood and the aortic root tissue were collected and serum lipid level was detected by biochemical analyzer, the serum IL-1β,IL-10 concentration was detected by ELISA, the lipid deposition of aortic tissue was observed by oil red O staining, CD<sub>68</sub><sup>+</sup>macrophage aggregation of aortic tissue was observed by immunohistochemical staining.Results:In the model group, TC and LDL-C were significantly increased, HDL-C was slightly reduced, and IL-1β,IL-10 were also increased significantly (<italic>P</italic><0.05).Aortic tissue oil red O positive staining cells increased significantly (<italic>P</italic><0.05),plaque lipid deposition was evident. The dots or patches protrude into the vascular and the vascular cavity was markedly narrowed, CD<sub>68</sub><sup>+</sup>macrophage was also increased significantly in aortic tissue (all <italic>P</italic><0.05).Compared to the model group, TC, LDL-C and IL-1β,IL-10 were significantly reduced (<italic>P</italic><0.05),HDL-C was slightly increased in both the simvastatin group and the total saponins group (<italic>P</italic><0.05).Positive staining cells of oil red O in aortic tissue, were significantly reduced, plaque lipid deposition and the area decreased. The degree of convexity was significantly reduced. CD<sub>68</sub><sup>+</sup>macrophage number and the macrophage aggregation were significantly reduced (all <italic>P</italic><0.05).Conclusion:PNS can regulate blood lipids, inhibit the immune inflammatory response, inhibit the lipid deposition of aortainhibiting and macrophage aggregation, which might be one of the anti-atherosclerosis mechanisms.http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2018.03027atherosclerosislipid depositiontotal saponins of panax notoginsengapolipoprotein Eknockout |
| spellingShingle | Changgeng FU Ruina BAI Yue LIU Linzi LONG Keji CHEN Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice 康复学报 atherosclerosis lipid deposition total saponins of panax notoginseng apolipoprotein E knockout |
| title | Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice |
| title_full | Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice |
| title_fullStr | Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice |
| title_full_unstemmed | Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice |
| title_short | Effect of Total Saponins of Panax Notoginseng on Lipids Deposition of Apolipoprotein E-Knockout Mice |
| title_sort | effect of total saponins of panax notoginseng on lipids deposition of apolipoprotein e knockout mice |
| topic | atherosclerosis lipid deposition total saponins of panax notoginseng apolipoprotein E knockout |
| url | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2018.03027 |
| work_keys_str_mv | AT changgengfu effectoftotalsaponinsofpanaxnotoginsengonlipidsdepositionofapolipoproteineknockoutmice AT ruinabai effectoftotalsaponinsofpanaxnotoginsengonlipidsdepositionofapolipoproteineknockoutmice AT yueliu effectoftotalsaponinsofpanaxnotoginsengonlipidsdepositionofapolipoproteineknockoutmice AT linzilong effectoftotalsaponinsofpanaxnotoginsengonlipidsdepositionofapolipoproteineknockoutmice AT kejichen effectoftotalsaponinsofpanaxnotoginsengonlipidsdepositionofapolipoproteineknockoutmice |