Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinoma
BackgroundLong non-coding RNAs (lncRNAs) are dysregulated in nasopharyngeal carcinoma (NPC), yet their interplay with pharmacological agents like aloe-emodin (AE) remains unclear. This study explores AE’s anti-NPC mechanisms via lncRNA D63785 and the PI3K/Akt/mTOR pathway.MethodsNPC cells (CNE1, C66...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1573408/full |
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| author | Min He Lei Xie Lei Xie Jiayi Huang Han Su Jiahua Hu Liuping Xie Liuping Xie Mengqin Li Mengqin Li Xin Zeng Xin Zeng Jianhong Tang Jianhong Tang Jianhong Tang |
| author_facet | Min He Lei Xie Lei Xie Jiayi Huang Han Su Jiahua Hu Liuping Xie Liuping Xie Mengqin Li Mengqin Li Xin Zeng Xin Zeng Jianhong Tang Jianhong Tang Jianhong Tang |
| author_sort | Min He |
| collection | DOAJ |
| description | BackgroundLong non-coding RNAs (lncRNAs) are dysregulated in nasopharyngeal carcinoma (NPC), yet their interplay with pharmacological agents like aloe-emodin (AE) remains unclear. This study explores AE’s anti-NPC mechanisms via lncRNA D63785 and the PI3K/Akt/mTOR pathway.MethodsNPC cells (CNE1, C666-1) were treated with AE, followed by qRT-PCR and Western blotting to assess lncRNA D63785 and PI3K/Akt/mTOR pathway proteins. siRNA-mediated lncRNA D63785 knockdown combined with functional assays (CCK-8, EdU, colony/wound-healing) evaluated AE’s effects on proliferation, migration, and pathway activity. In vivo validation used nude mouse xenografts.ResultsLncRNA D63785 was overexpressed in NPC cells (p < 0.01). AE suppressed lncRNA D63785 expression, concurrently reducing PI3K/Akt/mTOR phosphorylation (p < 0.05). siRNA knockdown partially reversed AE’s inhibition of NPC cell viability, proliferation, and migration. In vivo, AE attenuated tumor growth (p < 0.05), correlating with lncRNA D63785 downregulation and PI3K/Akt/mTOR dephosphorylation.ConclusionAE exerts anti-NPC effects by targeting the lncRNA D63785-PI3K/Akt/mTOR axis, offering a novel therapeutic strategy. These findings bridge AE’s pharmacological activity with lncRNA regulatory networks in NPC pathogenesis. |
| format | Article |
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| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-9532f5af9f6a4f24aa99b5b8aeb23dba2025-08-20T03:51:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-07-011610.3389/fphar.2025.15734081573408Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinomaMin He0Lei Xie1Lei Xie2Jiayi Huang3Han Su4Jiahua Hu5Liuping Xie6Liuping Xie7Mengqin Li8Mengqin Li9Xin Zeng10Xin Zeng11Jianhong Tang12Jianhong Tang13Jianhong Tang14Department of Pharmacy, The Second Affiliated Hospital of Guilin Medical University, Guilin, ChinaCollege of Pharmacy, Guilin Medical University, Guilin, ChinaGuangxi Key Laboratory of Diabetic Systems Medicine, Guilin Medical University, Guilin, ChinaDepartment of Traditional Chinese Medicine, The Second Affiliated Hospital of Guilin Medical University, Guilin, ChinaDepartment of Neurology, The Affiliated Hospital of Guilin Medical University, Guilin, ChinaCentral Laboratory, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, The Second Affiliated Hospital of Guilin Medical University, Guilin, ChinaCollege of Pharmacy, Guilin Medical University, Guilin, ChinaGuangxi Key Laboratory of Diabetic Systems Medicine, Guilin Medical University, Guilin, ChinaCollege of Pharmacy, Guilin Medical University, Guilin, ChinaGuangxi Key Laboratory of Diabetic Systems Medicine, Guilin Medical University, Guilin, ChinaCollege of Pharmacy, Guilin Medical University, Guilin, ChinaGuangxi Key Laboratory of Diabetic Systems Medicine, Guilin Medical University, Guilin, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Guilin Medical University, Guilin, ChinaGuangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, The Second Affiliated Hospital of Guilin Medical University, Guilin, ChinaSchool of General Medical, Guilin Medical University, Guilin, ChinaBackgroundLong non-coding RNAs (lncRNAs) are dysregulated in nasopharyngeal carcinoma (NPC), yet their interplay with pharmacological agents like aloe-emodin (AE) remains unclear. This study explores AE’s anti-NPC mechanisms via lncRNA D63785 and the PI3K/Akt/mTOR pathway.MethodsNPC cells (CNE1, C666-1) were treated with AE, followed by qRT-PCR and Western blotting to assess lncRNA D63785 and PI3K/Akt/mTOR pathway proteins. siRNA-mediated lncRNA D63785 knockdown combined with functional assays (CCK-8, EdU, colony/wound-healing) evaluated AE’s effects on proliferation, migration, and pathway activity. In vivo validation used nude mouse xenografts.ResultsLncRNA D63785 was overexpressed in NPC cells (p < 0.01). AE suppressed lncRNA D63785 expression, concurrently reducing PI3K/Akt/mTOR phosphorylation (p < 0.05). siRNA knockdown partially reversed AE’s inhibition of NPC cell viability, proliferation, and migration. In vivo, AE attenuated tumor growth (p < 0.05), correlating with lncRNA D63785 downregulation and PI3K/Akt/mTOR dephosphorylation.ConclusionAE exerts anti-NPC effects by targeting the lncRNA D63785-PI3K/Akt/mTOR axis, offering a novel therapeutic strategy. These findings bridge AE’s pharmacological activity with lncRNA regulatory networks in NPC pathogenesis.https://www.frontiersin.org/articles/10.3389/fphar.2025.1573408/fullAENPCLncRNA D63785PI3K/Akt/mTOR signaling pathwayproliferation and migration |
| spellingShingle | Min He Lei Xie Lei Xie Jiayi Huang Han Su Jiahua Hu Liuping Xie Liuping Xie Mengqin Li Mengqin Li Xin Zeng Xin Zeng Jianhong Tang Jianhong Tang Jianhong Tang Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinoma Frontiers in Pharmacology AE NPC LncRNA D63785 PI3K/Akt/mTOR signaling pathway proliferation and migration |
| title | Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinoma |
| title_full | Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinoma |
| title_fullStr | Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinoma |
| title_full_unstemmed | Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinoma |
| title_short | Aloe-emodin mediates the inhibitory effect of LncRNA D63785 on the PI3K/Akt/mTOR pathway in nasopharyngeal carcinoma |
| title_sort | aloe emodin mediates the inhibitory effect of lncrna d63785 on the pi3k akt mtor pathway in nasopharyngeal carcinoma |
| topic | AE NPC LncRNA D63785 PI3K/Akt/mTOR signaling pathway proliferation and migration |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1573408/full |
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