Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties
The production of metal nanoparticles is an expanding field of study owing to its promise in advancing technologies. Typically, these nanoparticles are created through chemical processes that are often detrimental to the environment. Globally large amounts of fruit waste are generated daily. Utilizi...
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Elsevier
2025-03-01
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| Series: | Hybrid Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773207X24002367 |
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| author | Pranay Pramanik Krishnendu Dakua Trisha Kar Ranabir Sahu Tarun Kumar Dua Gouranga Nandi Sangita Dey Anoop Kumar Ritu Khanra Paramita Paul |
| author_facet | Pranay Pramanik Krishnendu Dakua Trisha Kar Ranabir Sahu Tarun Kumar Dua Gouranga Nandi Sangita Dey Anoop Kumar Ritu Khanra Paramita Paul |
| author_sort | Pranay Pramanik |
| collection | DOAJ |
| description | The production of metal nanoparticles is an expanding field of study owing to its promise in advancing technologies. Typically, these nanoparticles are created through chemical processes that are often detrimental to the environment. Globally large amounts of fruit waste are generated daily. Utilizing these fruit waste to create metal nanoparticles through environmentally friendly methods presents an innovative, cost-efficient, and eco-friendly approach compared to conventional techniques which are energy intensive, costly and hazardous for the environment. Mango seed kernel extract and copper sulphate pentahydrate were used to produce copper oxide nanoparticles (CONPs) using a green synthesis method. Physico-chemical characterizations of the CONPs were done by DLS, FTIR, UV–Visible spectrophotometry, SEM, TEM, EDX and X-ray diffraction. The nanoparticles showed surface plasmon resonance peak at 276 nm, and were found to be of size 211 nm. FTIR analysis inveterate the occurrence of phytochemicals in both the mango seed kernel extract as well as the nanoparticles, suggesting the kernel's potential as stabilizers, reducers, and capping agents. DPPH free radical scavenging assay indicated the CONPs to have mild antioxidant activity with IC50 of 453.72 μg/mL. The synthesised CONPs were found to successfully inhibit growth of both Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. Moreover, MTT assay exhibited strong anticancer potential of CONPs against SH-SY5Y neuroblastoma cell-lines with IC50 of 24.14 μg/mL. |
| format | Article |
| id | doaj-art-9509c2c828a744d693017570a25ffac8 |
| institution | Kabale University |
| issn | 2773-207X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hybrid Advances |
| spelling | doaj-art-9509c2c828a744d693017570a25ffac82025-01-09T06:17:06ZengElsevierHybrid Advances2773-207X2025-03-018100375Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological propertiesPranay Pramanik0Krishnendu Dakua1Trisha Kar2Ranabir Sahu3Tarun Kumar Dua4Gouranga Nandi5Sangita Dey6Anoop Kumar7Ritu Khanra8Paramita Paul9Department of Pharmaceutical Technology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Pharmaceutical Technology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Pharmaceutical Technology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Pharmaceutical Technology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Pharmaceutical Technology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Pharmaceutical Technology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Biotechnology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Biotechnology, University of North Bengal, 734013, Darjeeling, West Bengal, IndiaDepartment of Pharmaceutical Technology, JIS University, Kolkata, West Bengal, IndiaDepartment of Pharmaceutical Technology, University of North Bengal, 734013, Darjeeling, West Bengal, India; Corresponding author. Department of Pharmaceutical Technology, University of North Bengal, Raja Rammohunpur, 734013, Darjeeling, West Bengal, India.The production of metal nanoparticles is an expanding field of study owing to its promise in advancing technologies. Typically, these nanoparticles are created through chemical processes that are often detrimental to the environment. Globally large amounts of fruit waste are generated daily. Utilizing these fruit waste to create metal nanoparticles through environmentally friendly methods presents an innovative, cost-efficient, and eco-friendly approach compared to conventional techniques which are energy intensive, costly and hazardous for the environment. Mango seed kernel extract and copper sulphate pentahydrate were used to produce copper oxide nanoparticles (CONPs) using a green synthesis method. Physico-chemical characterizations of the CONPs were done by DLS, FTIR, UV–Visible spectrophotometry, SEM, TEM, EDX and X-ray diffraction. The nanoparticles showed surface plasmon resonance peak at 276 nm, and were found to be of size 211 nm. FTIR analysis inveterate the occurrence of phytochemicals in both the mango seed kernel extract as well as the nanoparticles, suggesting the kernel's potential as stabilizers, reducers, and capping agents. DPPH free radical scavenging assay indicated the CONPs to have mild antioxidant activity with IC50 of 453.72 μg/mL. The synthesised CONPs were found to successfully inhibit growth of both Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. Moreover, MTT assay exhibited strong anticancer potential of CONPs against SH-SY5Y neuroblastoma cell-lines with IC50 of 24.14 μg/mL.http://www.sciencedirect.com/science/article/pii/S2773207X24002367Green synthesisCopper oxide nanoparticlesRaman spectraAntioxidant activityAntibacterial activityCytotoxic activity |
| spellingShingle | Pranay Pramanik Krishnendu Dakua Trisha Kar Ranabir Sahu Tarun Kumar Dua Gouranga Nandi Sangita Dey Anoop Kumar Ritu Khanra Paramita Paul Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties Hybrid Advances Green synthesis Copper oxide nanoparticles Raman spectra Antioxidant activity Antibacterial activity Cytotoxic activity |
| title | Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties |
| title_full | Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties |
| title_fullStr | Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties |
| title_full_unstemmed | Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties |
| title_short | Biosynthesis and in-vitro characterizations of copper oxide nanoparticle using Mangifera indica seed kernel extract and assessment of pharmacological properties |
| title_sort | biosynthesis and in vitro characterizations of copper oxide nanoparticle using mangifera indica seed kernel extract and assessment of pharmacological properties |
| topic | Green synthesis Copper oxide nanoparticles Raman spectra Antioxidant activity Antibacterial activity Cytotoxic activity |
| url | http://www.sciencedirect.com/science/article/pii/S2773207X24002367 |
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