Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers

Simultaneous multigene integration in Aspergillus fumigatus using CRISPR/Cas9 and endogenous counter-selectable markers

Abstract Background The discovery of CRISPR/Cas9 and its subsequent accessibility in daily research initiated a new era in genome editing. This game-changing genetic instrument enabled a vast array of challenging applications requiring site-specific genome engineering as well as applications involvi...

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Main Authors: Luis Enrique Sastré-Velásquez, Natalia Mach, Birte Mertens, Alexander Kühbacher, Petra Merschak, Alex Dallemulle, Lukas Lechner, Clara Baldin, George Diallinas, Fabio Gsaller
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Journal of Biological Engineering
Subjects:
Aspergillus fumigatus
CRISPR/Cas9
Endogenous counter-selectable markers
Multigene integration
Azole resistance
Online Access:https://doi.org/10.1186/s13036-025-00539-3
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https://doi.org/10.1186/s13036-025-00539-3

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