Imaging Reactive Oxygen Species in Arthritis
Reactive oxygen species (ROS) have been shown to play a role in the pathogenesis of arthritides. Luminol was used as the primary reporter of ROS and photons resulting from the chemiluminescence reaction were detected using a super-cooled CCD photon counting system. Luminol was injected intravenously...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2004-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200404124 |
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| _version_ | 1841564556654018560 |
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| author | Wei-Tsung Chen Ching-Hsuan Tung Ralph Weissleder |
| author_facet | Wei-Tsung Chen Ching-Hsuan Tung Ralph Weissleder |
| author_sort | Wei-Tsung Chen |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) have been shown to play a role in the pathogenesis of arthritides. Luminol was used as the primary reporter of ROS and photons resulting from the chemiluminescence reaction were detected using a super-cooled CCD photon counting system. Luminol was injected intravenously into groups of animals with different models of arthritis. Imaging signal correlated well with the severity of arthritis in focal and pan-arthritis as determined by histological measurement of ROS by formazan. Measurements were highly reproducible, sensitive, and repeatable. In vivo chemiluminescence imaging is expected to become a useful modality to elucidate the role of ROS in the pathogenesis of arthritides and in determining therapeutic efficacy of protective therapies. |
| format | Article |
| id | doaj-art-94959dc307f54fbcaff128b9fb3bfdd3 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2004-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-94959dc307f54fbcaff128b9fb3bfdd32025-01-02T22:37:31ZengSAGE PublishingMolecular Imaging1536-01212004-07-01310.1162/1535350020040412410.1162_15353500200404124Imaging Reactive Oxygen Species in ArthritisWei-Tsung ChenChing-Hsuan TungRalph WeisslederReactive oxygen species (ROS) have been shown to play a role in the pathogenesis of arthritides. Luminol was used as the primary reporter of ROS and photons resulting from the chemiluminescence reaction were detected using a super-cooled CCD photon counting system. Luminol was injected intravenously into groups of animals with different models of arthritis. Imaging signal correlated well with the severity of arthritis in focal and pan-arthritis as determined by histological measurement of ROS by formazan. Measurements were highly reproducible, sensitive, and repeatable. In vivo chemiluminescence imaging is expected to become a useful modality to elucidate the role of ROS in the pathogenesis of arthritides and in determining therapeutic efficacy of protective therapies.https://doi.org/10.1162/15353500200404124 |
| spellingShingle | Wei-Tsung Chen Ching-Hsuan Tung Ralph Weissleder Imaging Reactive Oxygen Species in Arthritis Molecular Imaging |
| title | Imaging Reactive Oxygen Species in Arthritis |
| title_full | Imaging Reactive Oxygen Species in Arthritis |
| title_fullStr | Imaging Reactive Oxygen Species in Arthritis |
| title_full_unstemmed | Imaging Reactive Oxygen Species in Arthritis |
| title_short | Imaging Reactive Oxygen Species in Arthritis |
| title_sort | imaging reactive oxygen species in arthritis |
| url | https://doi.org/10.1162/15353500200404124 |
| work_keys_str_mv | AT weitsungchen imagingreactiveoxygenspeciesinarthritis AT chinghsuantung imagingreactiveoxygenspeciesinarthritis AT ralphweissleder imagingreactiveoxygenspeciesinarthritis |