<span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i>
<span style="font-variant: small-caps;">d</span>-Xylitol is a low-calorie and anti-cariogenic sweetener suitable for diabetic patients, making it a valuable ingredient in various health-related applications. In this study, we investigated the production of <span style="...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/12/12/2489 |
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| Summary: | <span style="font-variant: small-caps;">d</span>-Xylitol is a low-calorie and anti-cariogenic sweetener suitable for diabetic patients, making it a valuable ingredient in various health-related applications. In this study, we investigated the production of <span style="font-variant: small-caps;">d</span>-xylitol from<span style="font-variant: small-caps;"> l</span>-arabinose derived from sugar beet press pulp (SBPP) hydrolysate using an engineered <i>Aspergillus niger</i> strain. Initial batch studies applying stirred tank bioreactors demonstrated <span style="font-variant: small-caps;">d</span>-xylitol production of 4.6 g L<sup>−1</sup> with a yield of 0.37 g <span style="font-variant: small-caps;">d</span>-xylitol g<sup>−1</sup> <span style="font-variant: small-caps;">l</span>-arabinose with a synthetic medium. Subsequently, the conversion of enzymatically produced and clarified SBPP hydrolysate was studied. We found that pre-treatment of the enzymatic hydrolysate with activated carbon was essential to remove inhibitory components. Moreover, an automated aeration switch-off was implemented based on the CO<sub>2</sub> signal of the off-gas analyzer of the stirred tank bioreactor to prevent <span style="font-variant: small-caps;">d</span>-xylitol degradation after <span style="font-variant: small-caps;">l</span>-arabinose depletion. This resulted in a final <span style="font-variant: small-caps;">d</span>-xylitol concentration of 4.3 g L<sup>−1</sup> with an improved yield of 0.43 g <span style="font-variant: small-caps;">d</span>-xylitol g<sup>−1</sup> <span style="font-variant: small-caps;">l</span>-arabinose. The feasibility of utilizing the agricultural residue SBPP for <span style="font-variant: small-caps;">d</span>-xylitol production was successfully demonstrated with engineered <i>A. niger</i>. |
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| ISSN: | 2076-2607 |