Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as Adsorbent
This research is aimed at evaluating strategies for the adsorption and recovery of anthocyanins present in purple cabbage using natural and modified clays as adsorbent. In the batch adsorption experiments, the anthocyanin extracts were put in contact with the adsorbents, and different parameters wer...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2023-01-01
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| Series: | Adsorption Science & Technology |
| Online Access: | http://dx.doi.org/10.1155/2023/2724122 |
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| author | Darlyson Tavares Guimarães Liana Maria Ramos Mendes Luiz Bruno de Sousa Sabino Edy Sousa de Brito Enrique Vilarrasa-García Enrique Rodríguez-Castellón Juan Antonio Cecilia Ivanildo José da Silva Junior |
| author_facet | Darlyson Tavares Guimarães Liana Maria Ramos Mendes Luiz Bruno de Sousa Sabino Edy Sousa de Brito Enrique Vilarrasa-García Enrique Rodríguez-Castellón Juan Antonio Cecilia Ivanildo José da Silva Junior |
| author_sort | Darlyson Tavares Guimarães |
| collection | DOAJ |
| description | This research is aimed at evaluating strategies for the adsorption and recovery of anthocyanins present in purple cabbage using natural and modified clays as adsorbent. In the batch adsorption experiments, the anthocyanin extracts were put in contact with the adsorbents, and different parameters were evaluated to determine the best conditions for their adsorption and recovery. It was noted that the highest levels of adsorption (28.0 mg g-1) occurred using a porous clay heterostructure (PCH) material as adsorbent, with a mass of 25 mg and 120 min of contact. Under the same conditions, the sepiolite only presented an adsorption capacity of 14.0 mg g-1. The desorption results showed that the 60% methanolic solution recovered 60% of the anthocyanins adsorbed on PCH, while the 80% ethanolic solution recovered 35% of those adsorbed on sepiolite. The eluted anthocyanin solutions showed a 98% lower sugar concentration than the crude extract, indicating the low affinity of the adsorbents for sugars. Six types of acylated cyanidins were identified via UPLC-QToF-MSE in the extract, and it was confirmed from the FTIR analyses that the highest affinity of the clays occurred with the anthocyanins that presented more organic acid in their structure. The results show that PCH and sepiolite have high selectivity for anthocyanins and low affinity for the sugars present in the plant extract, facilitating the process of partial purification and application of these pigments. |
| format | Article |
| id | doaj-art-94097453cafe4534980f858743fb536f |
| institution | Kabale University |
| issn | 2048-4038 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-94097453cafe4534980f858743fb536f2025-01-02T22:22:32ZengSAGE PublishingAdsorption Science & Technology2048-40382023-01-01202310.1155/2023/2724122Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as AdsorbentDarlyson Tavares Guimarães0Liana Maria Ramos Mendes1Luiz Bruno de Sousa Sabino2Edy Sousa de Brito3Enrique Vilarrasa-García4Enrique Rodríguez-Castellón5Juan Antonio Cecilia6Ivanildo José da Silva Junior7Department of Chemical EngineeringDepartment of Chemical EngineeringDepartment of Chemical EngineeringEmbrapa Tropical AgroindustryDepartment of Chemical EngineeringDepartment of Inorganic ChemistryDepartment of Inorganic ChemistryDepartment of Chemical EngineeringThis research is aimed at evaluating strategies for the adsorption and recovery of anthocyanins present in purple cabbage using natural and modified clays as adsorbent. In the batch adsorption experiments, the anthocyanin extracts were put in contact with the adsorbents, and different parameters were evaluated to determine the best conditions for their adsorption and recovery. It was noted that the highest levels of adsorption (28.0 mg g-1) occurred using a porous clay heterostructure (PCH) material as adsorbent, with a mass of 25 mg and 120 min of contact. Under the same conditions, the sepiolite only presented an adsorption capacity of 14.0 mg g-1. The desorption results showed that the 60% methanolic solution recovered 60% of the anthocyanins adsorbed on PCH, while the 80% ethanolic solution recovered 35% of those adsorbed on sepiolite. The eluted anthocyanin solutions showed a 98% lower sugar concentration than the crude extract, indicating the low affinity of the adsorbents for sugars. Six types of acylated cyanidins were identified via UPLC-QToF-MSE in the extract, and it was confirmed from the FTIR analyses that the highest affinity of the clays occurred with the anthocyanins that presented more organic acid in their structure. The results show that PCH and sepiolite have high selectivity for anthocyanins and low affinity for the sugars present in the plant extract, facilitating the process of partial purification and application of these pigments.http://dx.doi.org/10.1155/2023/2724122 |
| spellingShingle | Darlyson Tavares Guimarães Liana Maria Ramos Mendes Luiz Bruno de Sousa Sabino Edy Sousa de Brito Enrique Vilarrasa-García Enrique Rodríguez-Castellón Juan Antonio Cecilia Ivanildo José da Silva Junior Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as Adsorbent Adsorption Science & Technology |
| title | Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as Adsorbent |
| title_full | Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as Adsorbent |
| title_fullStr | Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as Adsorbent |
| title_full_unstemmed | Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as Adsorbent |
| title_short | Partial Purification of Anthocyanins (Brassica oleracea var. Rubra) from Purple Cabbage Using Natural and Modified Clays as Adsorbent |
| title_sort | partial purification of anthocyanins brassica oleracea var rubra from purple cabbage using natural and modified clays as adsorbent |
| url | http://dx.doi.org/10.1155/2023/2724122 |
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