LncRNA DRAIC facilitates the progression of renal cell carcinoma by modulting the microRNA-145-3p/ABRACL pathway signaling
Abstract This study aims to elucidate the functional mechanisms of LncRNA DRAIC in renal cell carcinoma, particularly its regulation of tumor progression via microRNA-145-3p and ABRACL. The downstream targets of LncRNA DRAIC were predicted using databases, and their interactions with microRNA-145-3p...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-08070-7 |
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| Summary: | Abstract This study aims to elucidate the functional mechanisms of LncRNA DRAIC in renal cell carcinoma, particularly its regulation of tumor progression via microRNA-145-3p and ABRACL. The downstream targets of LncRNA DRAIC were predicted using databases, and their interactions with microRNA-145-3p were experimentally validated. The effects of LncRNA DRAIC overexpression on the proliferation, invasion, and migration of renal carcinoma cells were evaluated using stable cell lines, CCK-8 assays, Transwell assays, subcutaneous tumor transplantation in nude mice, and in vivo fluorescence imaging experiments. Techniques such as Western blot and RT-PCR were utilized to monitor the expression levels of ABRACL under the regulation of LncRNA DRAIC, further confirming its role in renal carcinoma cell functions. Overexpression of LncRNA DRAIC significantly enhanced the proliferation, invasion, and migration capabilities of renal carcinoma cells. Moreover, it was discovered that LncRNA DRAIC regulates the expression of ABRACL by modulating microRNA-145-3p, thereby affecting the functionality of renal carcinoma cells. This reveals the critical mechanisms of LncRNA DRAIC in renal cell carcinoma, where it governs tumor cell biology through microRNA-145-3p and ABRACL, offering new targets and theoretical foundations for the treatment of renal cell carcinoma. |
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| ISSN: | 2045-2322 |