Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology
Abstract Background Infertility is a reproductive health problem that attracts worldwide attention. Polycystic ovary syndrome (PCOS) is a major cause of female infertility and patients with obesity and PCOS are particularly common in clinical practice. Long non-coding RNA (lncRNAs) are a functional...
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BMC
2024-11-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | https://doi.org/10.1186/s13048-024-01552-8 |
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| author | Xin Xin Li Dong Jiaxi Li Wen Chen Yue Qiu Fang Lian Haicui Wu |
| author_facet | Xin Xin Li Dong Jiaxi Li Wen Chen Yue Qiu Fang Lian Haicui Wu |
| author_sort | Xin Xin |
| collection | DOAJ |
| description | Abstract Background Infertility is a reproductive health problem that attracts worldwide attention. Polycystic ovary syndrome (PCOS) is a major cause of female infertility and patients with obesity and PCOS are particularly common in clinical practice. Long non-coding RNA (lncRNAs) are a functional core in cells that regulate gene expression, transcription, and chromatin modification processes, and participate in epigenetics, cell cycle, and cell differentiation. LncRNAs are assumed to play a role in the occurrence and development of PCOS; however, their specific mechanism of action remains to be elucidated. Methods High-throughput sequencing technology has been used to sequence and analyze lncRNAs in exosomes from the follicular fluid of patients with obesity and PCOS and those who underwent assisted reproductive therapy owing to male factors. Specific expression profiles of patients with obesity and PCOS were obtained and functional information analysis combined with a literature review were performed to screen for differentially expressed lncRNAs, which were validated using real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results High-throughput sequencing analysis revealed that compared to normal patients with male infertility, patients with obesity and PCOS had a total of 20 lncRNAs with significant expression differences in follicular fluid exosomes. Among them, 17 lncRNAs were upregulated and three were downregulated. Functional analysis showed that differentially expressed genes were mainly enriched in “cell metabolism,” “cell adhesion,” and other aspects: related gene pathways mainly involved Huntington’s disease, Parkinson’s disease, spliceosomes, non-alcoholic fatty liver disease, and ribosomes. Verification of differentially expressed lncRNAs revealed that the expression of lncRNAs TPT1-AS1, PTOV1-AS1, PTPRG-AS1, and SNHG14 in follicular fluid exosomes was consistent with the sequencing results. Conclusion A preliminary differential expression profile of lncRNAs in exosomes of patients with obesity and PCOS was established by transcriptomic analysis of these individuals. Our bioinformatics analysis results may be applicable to further study of the impact mechanism involving obesity and PCOS. These differentially expressed lncRNAs maybe served as potential biomarkers for in-depth studies of the occurrence, development on Follicle quality and function for patients with PCOS in the future. |
| format | Article |
| id | doaj-art-93d40723b50b4e2da5e8d50de3021be5 |
| institution | Kabale University |
| issn | 1757-2215 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj-art-93d40723b50b4e2da5e8d50de3021be52024-11-17T12:42:18ZengBMCJournal of Ovarian Research1757-22152024-11-0117111310.1186/s13048-024-01552-8Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technologyXin Xin0Li Dong1Jiaxi Li2Wen Chen3Yue Qiu4Fang Lian5Haicui Wu6First School of Clinical Medicine, Shandong University of Traditional Chinese MedicineFirst School of Clinical Medicine, Shandong University of Traditional Chinese MedicineShandong Wendeng Osteopathic HospitalFirst School of Clinical Medicine, Shandong University of Traditional Chinese MedicineAffiliated Hospital of Shandong University of Traditional Chinese MedicineAffiliated Hospital of Shandong University of Traditional Chinese MedicineAffiliated Hospital of Shandong University of Traditional Chinese MedicineAbstract Background Infertility is a reproductive health problem that attracts worldwide attention. Polycystic ovary syndrome (PCOS) is a major cause of female infertility and patients with obesity and PCOS are particularly common in clinical practice. Long non-coding RNA (lncRNAs) are a functional core in cells that regulate gene expression, transcription, and chromatin modification processes, and participate in epigenetics, cell cycle, and cell differentiation. LncRNAs are assumed to play a role in the occurrence and development of PCOS; however, their specific mechanism of action remains to be elucidated. Methods High-throughput sequencing technology has been used to sequence and analyze lncRNAs in exosomes from the follicular fluid of patients with obesity and PCOS and those who underwent assisted reproductive therapy owing to male factors. Specific expression profiles of patients with obesity and PCOS were obtained and functional information analysis combined with a literature review were performed to screen for differentially expressed lncRNAs, which were validated using real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results High-throughput sequencing analysis revealed that compared to normal patients with male infertility, patients with obesity and PCOS had a total of 20 lncRNAs with significant expression differences in follicular fluid exosomes. Among them, 17 lncRNAs were upregulated and three were downregulated. Functional analysis showed that differentially expressed genes were mainly enriched in “cell metabolism,” “cell adhesion,” and other aspects: related gene pathways mainly involved Huntington’s disease, Parkinson’s disease, spliceosomes, non-alcoholic fatty liver disease, and ribosomes. Verification of differentially expressed lncRNAs revealed that the expression of lncRNAs TPT1-AS1, PTOV1-AS1, PTPRG-AS1, and SNHG14 in follicular fluid exosomes was consistent with the sequencing results. Conclusion A preliminary differential expression profile of lncRNAs in exosomes of patients with obesity and PCOS was established by transcriptomic analysis of these individuals. Our bioinformatics analysis results may be applicable to further study of the impact mechanism involving obesity and PCOS. These differentially expressed lncRNAs maybe served as potential biomarkers for in-depth studies of the occurrence, development on Follicle quality and function for patients with PCOS in the future.https://doi.org/10.1186/s13048-024-01552-8Polycystic ovary syndromeLncRNAExosomeHigh throughput sequencingObesity |
| spellingShingle | Xin Xin Li Dong Jiaxi Li Wen Chen Yue Qiu Fang Lian Haicui Wu Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology Journal of Ovarian Research Polycystic ovary syndrome LncRNA Exosome High throughput sequencing Obesity |
| title | Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology |
| title_full | Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology |
| title_fullStr | Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology |
| title_full_unstemmed | Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology |
| title_short | Exploring lncRNA expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high-throughput sequencing technology |
| title_sort | exploring lncrna expression in follicular fluid exosomes of patients with obesity and polycystic ovary syndrome based on high throughput sequencing technology |
| topic | Polycystic ovary syndrome LncRNA Exosome High throughput sequencing Obesity |
| url | https://doi.org/10.1186/s13048-024-01552-8 |
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