Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis.
<h4>Background</h4>The emergence of drug-resistant Tuberculosis (TB) has made treatment challenging. Although fluoroquinolones (FQs) are used as key drugs in the treatment of multidrug-resistant tuberculosis (MDR-TB), the problem of FQs resistance is becoming increasingly serious. Rifamp...
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Public Library of Science (PLoS)
2025-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0315512 |
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| author | Qin Zhou Na Pu Ge Xu Hangchi Liu Xudong Jia Xiaomin Wang Peng Xu |
| author_facet | Qin Zhou Na Pu Ge Xu Hangchi Liu Xudong Jia Xiaomin Wang Peng Xu |
| author_sort | Qin Zhou |
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| description | <h4>Background</h4>The emergence of drug-resistant Tuberculosis (TB) has made treatment challenging. Although fluoroquinolones (FQs) are used as key drugs in the treatment of multidrug-resistant tuberculosis (MDR-TB), the problem of FQs resistance is becoming increasingly serious. Rifampicin (RIF) resistance is considered a risk factor for FQs resistance. The objective of this study was to investigate the impact of RIF and isoniazid (INH) resistance on the FQs resistance in vitro experiment.<h4>Methods</h4>FQs resistant strains were selected in vitro from RIF and/or INH resistant Mycobacterium smegmatis (M.sm). The sequencing of the gyrA gene, and the minimum inhibitory concentration (MIC) of FQs (ciprofloxacin, levofloxacin, moxifloxacin and gatifloxacin) were performed for FQs-resistant strains.<h4>Results</h4>A total of 222 FQs-resistant M.sm strains were selected, all of which had the gyrA mutation. Seven gyrA mutations were detected, with mutations at loci 90 and 94 being the most common. There were no differences in FQs resistance developed from RIF and/or INH resistant M.sm. There was a significant difference in the MIC of the gyrA mutant types to FQs. The highest resistance to FQs was observed in the Gly88Cys mutant strains. M.sm with the identical gyrA mutation showed the highest resistance to ciprofloxacin and relatively low resistance to gatifloxacin and moxifloxacin.<h4>Conclusions</h4>In this study, we found no evidence that RIF and/or INH resistance directly affects FQs resistance in M.sm in vitro experiments. Resistance profiles of different gryA mutations to the four FQs drugs were also presented. These findings provide a more comprehensive understanding of FQs resistance. |
| format | Article |
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| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-93c7f41eeb76494fb8a516e9e0b1e4432025-01-08T05:31:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031551210.1371/journal.pone.0315512Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis.Qin ZhouNa PuGe XuHangchi LiuXudong JiaXiaomin WangPeng Xu<h4>Background</h4>The emergence of drug-resistant Tuberculosis (TB) has made treatment challenging. Although fluoroquinolones (FQs) are used as key drugs in the treatment of multidrug-resistant tuberculosis (MDR-TB), the problem of FQs resistance is becoming increasingly serious. Rifampicin (RIF) resistance is considered a risk factor for FQs resistance. The objective of this study was to investigate the impact of RIF and isoniazid (INH) resistance on the FQs resistance in vitro experiment.<h4>Methods</h4>FQs resistant strains were selected in vitro from RIF and/or INH resistant Mycobacterium smegmatis (M.sm). The sequencing of the gyrA gene, and the minimum inhibitory concentration (MIC) of FQs (ciprofloxacin, levofloxacin, moxifloxacin and gatifloxacin) were performed for FQs-resistant strains.<h4>Results</h4>A total of 222 FQs-resistant M.sm strains were selected, all of which had the gyrA mutation. Seven gyrA mutations were detected, with mutations at loci 90 and 94 being the most common. There were no differences in FQs resistance developed from RIF and/or INH resistant M.sm. There was a significant difference in the MIC of the gyrA mutant types to FQs. The highest resistance to FQs was observed in the Gly88Cys mutant strains. M.sm with the identical gyrA mutation showed the highest resistance to ciprofloxacin and relatively low resistance to gatifloxacin and moxifloxacin.<h4>Conclusions</h4>In this study, we found no evidence that RIF and/or INH resistance directly affects FQs resistance in M.sm in vitro experiments. Resistance profiles of different gryA mutations to the four FQs drugs were also presented. These findings provide a more comprehensive understanding of FQs resistance.https://doi.org/10.1371/journal.pone.0315512 |
| spellingShingle | Qin Zhou Na Pu Ge Xu Hangchi Liu Xudong Jia Xiaomin Wang Peng Xu Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis. PLoS ONE |
| title | Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis. |
| title_full | Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis. |
| title_fullStr | Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis. |
| title_full_unstemmed | Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis. |
| title_short | Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis. |
| title_sort | rifampicin and isoniazid resistance not promote fluoroquinolone resistance in mycobacterium smegmatis |
| url | https://doi.org/10.1371/journal.pone.0315512 |
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