Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/Disorder
ABSTRACT The pancreas is involved in digestion and glucose regulation in the human body. Given the recognized link between chronic pancreatitis and pancreatic cancer, addressing pancreatic disorders and pancreatic cancer is particularly challenging. This review aims to highlight the limitations of t...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | JGH Open |
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| Online Access: | https://doi.org/10.1002/jgh3.70061 |
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| author | Suvro Biswas Shamima Afrose Mohasana Akter Mita Md. Robiul Hasan Mst. Sharmin Sultana Shimu Shahriar Zaman Md. Abu Saleh |
| author_facet | Suvro Biswas Shamima Afrose Mohasana Akter Mita Md. Robiul Hasan Mst. Sharmin Sultana Shimu Shahriar Zaman Md. Abu Saleh |
| author_sort | Suvro Biswas |
| collection | DOAJ |
| description | ABSTRACT The pancreas is involved in digestion and glucose regulation in the human body. Given the recognized link between chronic pancreatitis and pancreatic cancer, addressing pancreatic disorders and pancreatic cancer is particularly challenging. This review aims to highlight the limitations of traditional methods in diagnosing pancreatic disorders and cancer and explore several next‐generation sequencing (NGS) approaches as a promising alternative. There are distinct clinical symptoms that are shared by a number of clinical phenotypes of pancreatic illness induced by particular genetic mutations. Traditional diagnostic methods encompass computed tomography, magnetic resonance imaging, contrast‐enhanced Doppler ultrasound, endoscopic ultrasound, endoscopic retrograde cholangiopancreatography, transabdominal ultrasound, laparoscopy, and positron emission tomography have a prognostic ability of only 5% or less and a 5‐year survival rate. Genetic sequencing can be employed as an alternative to conventional diagnostic techniques. Sanger sequencing and NGS are currently largely operated genome analysis, with no exception for pancreatic disease diagnosis. The NGS methods can sequence millions to billions of short DNA fragments, enabling enormous sample screening in a short amount of time with low‐abundance detection, like in 0.1%–1% mutation prevalence declining approximate cost. Whole‐genome sequencing, whole‐exome sequencing, RNA sequencing, and single‐cell NGS are a few NGS methods utilized to diagnose pancreatic disease. For both research and clinical applications, the NGS techniques can provide a precise diagnosis of pancreatic disorders in a short amount of time at a reasonable expenditure. |
| format | Article |
| id | doaj-art-9375d2b434d94e868e52af453b650d97 |
| institution | Kabale University |
| issn | 2397-9070 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | JGH Open |
| spelling | doaj-art-9375d2b434d94e868e52af453b650d972024-11-28T07:56:34ZengWileyJGH Open2397-90702024-11-01811n/an/a10.1002/jgh3.70061Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/DisorderSuvro Biswas0Shamima Afrose1Mohasana Akter Mita2Md. Robiul Hasan3Mst. Sharmin Sultana Shimu4Shahriar Zaman5Md. Abu Saleh6Miocrobiology Laboratory, Department of Genetic Engineering and Biotechnology University of Rajshahi BangladeshDepartment of Genetic Engineering and Biotechnology University of Rajshahi Rajshahi BangladeshDepartment of Genetic Engineering and Biotechnology University of Rajshahi Rajshahi BangladeshDepartment of Genetic Engineering and Biotechnology University of Rajshahi Rajshahi BangladeshDepartment of Genetic Engineering and Biotechnology University of Rajshahi Rajshahi BangladeshMiocrobiology Laboratory, Department of Genetic Engineering and Biotechnology University of Rajshahi BangladeshMiocrobiology Laboratory, Department of Genetic Engineering and Biotechnology University of Rajshahi BangladeshABSTRACT The pancreas is involved in digestion and glucose regulation in the human body. Given the recognized link between chronic pancreatitis and pancreatic cancer, addressing pancreatic disorders and pancreatic cancer is particularly challenging. This review aims to highlight the limitations of traditional methods in diagnosing pancreatic disorders and cancer and explore several next‐generation sequencing (NGS) approaches as a promising alternative. There are distinct clinical symptoms that are shared by a number of clinical phenotypes of pancreatic illness induced by particular genetic mutations. Traditional diagnostic methods encompass computed tomography, magnetic resonance imaging, contrast‐enhanced Doppler ultrasound, endoscopic ultrasound, endoscopic retrograde cholangiopancreatography, transabdominal ultrasound, laparoscopy, and positron emission tomography have a prognostic ability of only 5% or less and a 5‐year survival rate. Genetic sequencing can be employed as an alternative to conventional diagnostic techniques. Sanger sequencing and NGS are currently largely operated genome analysis, with no exception for pancreatic disease diagnosis. The NGS methods can sequence millions to billions of short DNA fragments, enabling enormous sample screening in a short amount of time with low‐abundance detection, like in 0.1%–1% mutation prevalence declining approximate cost. Whole‐genome sequencing, whole‐exome sequencing, RNA sequencing, and single‐cell NGS are a few NGS methods utilized to diagnose pancreatic disease. For both research and clinical applications, the NGS techniques can provide a precise diagnosis of pancreatic disorders in a short amount of time at a reasonable expenditure.https://doi.org/10.1002/jgh3.70061IPMNLAPCMCNNGSpancreatic diseasepancreatitis |
| spellingShingle | Suvro Biswas Shamima Afrose Mohasana Akter Mita Md. Robiul Hasan Mst. Sharmin Sultana Shimu Shahriar Zaman Md. Abu Saleh Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/Disorder JGH Open IPMN LAPC MCN NGS pancreatic disease pancreatitis |
| title | Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/Disorder |
| title_full | Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/Disorder |
| title_fullStr | Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/Disorder |
| title_full_unstemmed | Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/Disorder |
| title_short | Next‐Generation Sequencing: An Advanced Diagnostic Tool for Detection of Pancreatic Disease/Disorder |
| title_sort | next generation sequencing an advanced diagnostic tool for detection of pancreatic disease disorder |
| topic | IPMN LAPC MCN NGS pancreatic disease pancreatitis |
| url | https://doi.org/10.1002/jgh3.70061 |
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