Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2
Recent advancements in green nanotechnology have enabled the synthesis of nanoparticles using natural resources, thereby offering ecofriendly alternatives for various biomedical applications. In this study, we report the biosynthesis of gold nanoparticles (GNPs) using vitalmelon (VW) extract (VW-GNP...
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Elsevier
2025-02-01
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| Series: | Journal of Photochemistry and Photobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666469024000290 |
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| author | Sun Young Park He mi Kang Kangmin Park Lu Guo Jin-Woo Oh Geuntae Park Nam Jun Kang Young-Whan Choi |
| author_facet | Sun Young Park He mi Kang Kangmin Park Lu Guo Jin-Woo Oh Geuntae Park Nam Jun Kang Young-Whan Choi |
| author_sort | Sun Young Park |
| collection | DOAJ |
| description | Recent advancements in green nanotechnology have enabled the synthesis of nanoparticles using natural resources, thereby offering ecofriendly alternatives for various biomedical applications. In this study, we report the biosynthesis of gold nanoparticles (GNPs) using vitalmelon (VW) extract (VW-GNPs) without the need for stabilizers or surfactants. The VW extract served as a reducing and a stabilizing agent. The synthesized VW-GNPs were characterized by UV–Vis spectroscopy, dynamic light scattering, Fourier-transform infrared spectroscopy, high-resolution transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The results revealed successful synthesis of VW-GNPs with an average size of 35.41 ± 2.37 nm and a zeta potential of -28.16 ± 1.01 mV, indicating good stability. The biological activity of VW-GNPs was evaluated in lipogenesis and browning of differentiating white adipocytes, focusing on their effects on lipid droplet formation, mitochondrial biogenesis, and expression of browning markers such as UCP1, PRDM16, and PGC1α. VW-GNPs significantly reduced lipid accumulation in fully differentiated 3T3-L1 adipocytes and promoted the conversion of white adipocytes into metabolically active beige adipocytes. Moreover, VW-GNPs enhanced mitochondrial biogenesis, which is crucial for energy expenditure in beige adipocytes. Further investigation using pharmacological inhibition and knockdown experiments revealed that phospholipase D2 inhibition synergistically promoted the browning effects of VW-GNPs. These findings suggest that VW-GNPs represent a promising novel therapeutic strategy to combat obesity by modulating adipocyte metabolism and increasing energy expenditure via adipocyte browning. |
| format | Article |
| id | doaj-art-92fe0e9480e74eb79f64724ff3765ee4 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Journal of Photochemistry and Photobiology |
| spelling | doaj-art-92fe0e9480e74eb79f64724ff3765ee42025-01-12T05:25:49ZengElsevierJournal of Photochemistry and Photobiology2666-46902025-02-0125100254Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2Sun Young Park0He mi Kang1Kangmin Park2Lu Guo3Jin-Woo Oh4Geuntae Park5Nam Jun Kang6Young-Whan Choi7Institute of Nanobio Convergence, Pusan National University, Busan 46241, Republic of Korea; Correspondence author at: Institute of Nanobio Convergence, Pusan National University, 2, Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea.Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of KoreaInstitute of Nanobio Convergence, Pusan National University, Busan 46241, Republic of KoreaDepartment of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of KoreaDepartment of Nano Fusion Technology, Graduate School, Pusan National University, Busan 46241, Republic of KoreaDepartment of Nano Fusion Technology, Graduate School, Pusan National University, Busan 46241, Republic of KoreaDepartment of Horticulture, Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 50463, Republic of Korea; Correspondence author at: Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, 1268-50, Samnangjin-ro, Samnangjin-eup, Miryang-si, Gyeongsangnam-do, Miryang 50463, Republic of Korea.Recent advancements in green nanotechnology have enabled the synthesis of nanoparticles using natural resources, thereby offering ecofriendly alternatives for various biomedical applications. In this study, we report the biosynthesis of gold nanoparticles (GNPs) using vitalmelon (VW) extract (VW-GNPs) without the need for stabilizers or surfactants. The VW extract served as a reducing and a stabilizing agent. The synthesized VW-GNPs were characterized by UV–Vis spectroscopy, dynamic light scattering, Fourier-transform infrared spectroscopy, high-resolution transmission electron microscopy, and energy-dispersive X-ray spectroscopy. The results revealed successful synthesis of VW-GNPs with an average size of 35.41 ± 2.37 nm and a zeta potential of -28.16 ± 1.01 mV, indicating good stability. The biological activity of VW-GNPs was evaluated in lipogenesis and browning of differentiating white adipocytes, focusing on their effects on lipid droplet formation, mitochondrial biogenesis, and expression of browning markers such as UCP1, PRDM16, and PGC1α. VW-GNPs significantly reduced lipid accumulation in fully differentiated 3T3-L1 adipocytes and promoted the conversion of white adipocytes into metabolically active beige adipocytes. Moreover, VW-GNPs enhanced mitochondrial biogenesis, which is crucial for energy expenditure in beige adipocytes. Further investigation using pharmacological inhibition and knockdown experiments revealed that phospholipase D2 inhibition synergistically promoted the browning effects of VW-GNPs. These findings suggest that VW-GNPs represent a promising novel therapeutic strategy to combat obesity by modulating adipocyte metabolism and increasing energy expenditure via adipocyte browning.http://www.sciencedirect.com/science/article/pii/S2666469024000290Green nanotechnologyGold nanoparticlesVitalmelonAdipocyte browningPhospholipase D2 |
| spellingShingle | Sun Young Park He mi Kang Kangmin Park Lu Guo Jin-Woo Oh Geuntae Park Nam Jun Kang Young-Whan Choi Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2 Journal of Photochemistry and Photobiology Green nanotechnology Gold nanoparticles Vitalmelon Adipocyte browning Phospholipase D2 |
| title | Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2 |
| title_full | Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2 |
| title_fullStr | Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2 |
| title_full_unstemmed | Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2 |
| title_short | Phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase D2 |
| title_sort | phytoreduced gold nanoparticles from vitalmelon promote white adipocyte browning in murine cells by targeting phospholipase d2 |
| topic | Green nanotechnology Gold nanoparticles Vitalmelon Adipocyte browning Phospholipase D2 |
| url | http://www.sciencedirect.com/science/article/pii/S2666469024000290 |
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