Effect of ergothineine and quercetin additions in to the in vitro embriyo development
<b>Aim:</b> This study aimed to investigate the effects of ergothioneine and quercetin on the in vitro development of bovine embryos.<p> <b>Materials and Methods: </b>Cumulus-oocyte complexes (COCs) were collected via ovary aspiration from a local abattoir and cultured...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2024-06-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1447 |
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| Summary: | <b>Aim:</b> This study aimed to investigate the effects of ergothioneine and
quercetin on the in vitro development of bovine embryos.<p>
<b>Materials and Methods: </b>Cumulus-oocyte complexes (COCs) were collected
via ovary aspiration from a local abattoir and cultured in vitro for maturation.
After maturation, in vitro fertilization was performed. The pronuclear
embryos were divided into three groups: control, 10 ?M L-ergothioneine,
and 10 ?M quercetin supplemented. After the addition of antioxidants to the
CR1aa medium, in vitro culture of embryos were performed. The cleavage
and morula rates were assessed on days 2 and 5, respectively. Blastocyst
formation and quality were assessed on days 7-8.<p>
<b>Results:</b> Statistical analysis showed cleavage and morula rates were
significantly higher in the ergothioneine group compared to the quercetin and
control groups (P<0.05). While no blastocysts formed in the quercetin group,
the blastocyst rate reached to 17.96% with ergothioneine supplementation
on day 8.<p>
<b>Conclusion: </b>In conclusion, supplementation with 10 ?M ergothioneine
enhanced the in vitro development of bovine oocytes. However, 10 ?M
quercetin supplementation impaired development, and no blastocyst
formation observed. Further studies utilizing different concentrations are
warranted to better understand the effects. This study provides insights into
modulating oxidative stress during in vitro embryonic production. |
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| ISSN: | 1309-6958 2146-1953 |