Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice
Abstract Saccharomyces boulardii (S. boulardii) is a fungal probiotic used to treat digestive disorders. However, the mechanism(s) by which S. boulardii affects the small intestine remains unclear. Here, we aimed to explore the effects of S. boulardii on the small intestine and the underlying mechan...
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2024-11-01
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| Online Access: | https://doi.org/10.1186/s12866-024-03647-0 |
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| author | Shuai Tang Jia Li Yi Li Haitao Du Wenya Zhu Ru Zhang Jun Wan |
| author_facet | Shuai Tang Jia Li Yi Li Haitao Du Wenya Zhu Ru Zhang Jun Wan |
| author_sort | Shuai Tang |
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| description | Abstract Saccharomyces boulardii (S. boulardii) is a fungal probiotic used to treat digestive disorders. However, the mechanism(s) by which S. boulardii affects the small intestine remains unclear. Here, we aimed to explore the effects of S. boulardii on the small intestine and the underlying mechanisms in mice with loperamide-induced constipation. While S. boulardii administration did not fully reverse the alterations in loperamide-induced defecation parameters, it altered the small intestinal floral composition toward a community conducive to alleviate constipation. Moreover, S. boulardii up-regulated the expression of tyrosine-protein kinase Kit (c-Kit), aquaporin 3 (AQP3), interleukin (IL)-10, myosin light chain kinase (MLCK), and phosphorylated myosin light chain 20 (P-MLC20), while concurrently down-regulating the expression levels of inducible nitric oxide synthase (iNOS), p65, and IL-17 A. These alterations indicate a discernible effect of small intestinal water reabsorption, inflammatory factor levels, and smooth muscle contraction. Saccharomyces boulardii also positively regulated small intestinal metabolite levels, such as fructose 6-phosphate, dihomo-alpha-linolenic acid, and 3-(4-hydroxyphenyl) lactate, and participated in metabolic pathways such as arginine biosynthesis, linoleic acid metabolism, and protein digestion and absorption. While not fully reversing defecation changes, Saccharomyces boulardii alters intestinal flora, up-regulates key proteins affecting water reabsorption and inflammation, and positively influences metabolic pathways. Our study provides serves as a basis for further studies on the application of S. boulardii in the treatment of intestinal disorders. |
| format | Article |
| id | doaj-art-912d8f4cd4734b9cb0b27e6e5ddc2307 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-912d8f4cd4734b9cb0b27e6e5ddc23072024-11-24T12:13:22ZengBMCBMC Microbiology1471-21802024-11-0124111310.1186/s12866-024-03647-0Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated miceShuai Tang0Jia Li1Yi Li2Haitao Du3Wenya Zhu4Ru Zhang5Jun Wan6Medical School of Chinese PLADepartment of Gastroenterology, The 983rd Hospital of Joint Logistic Support Force of PLADepartment of Gastroenterology, The First Medical Center, Chinese PLA General HospitalDepartment of Gastroenterology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General HospitalDepartment of Geriatrics, The Sixth Medical Center, Chinese PLA General HospitalDepartment of Gastroenterology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General HospitalDepartment of Gastroenterology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General HospitalAbstract Saccharomyces boulardii (S. boulardii) is a fungal probiotic used to treat digestive disorders. However, the mechanism(s) by which S. boulardii affects the small intestine remains unclear. Here, we aimed to explore the effects of S. boulardii on the small intestine and the underlying mechanisms in mice with loperamide-induced constipation. While S. boulardii administration did not fully reverse the alterations in loperamide-induced defecation parameters, it altered the small intestinal floral composition toward a community conducive to alleviate constipation. Moreover, S. boulardii up-regulated the expression of tyrosine-protein kinase Kit (c-Kit), aquaporin 3 (AQP3), interleukin (IL)-10, myosin light chain kinase (MLCK), and phosphorylated myosin light chain 20 (P-MLC20), while concurrently down-regulating the expression levels of inducible nitric oxide synthase (iNOS), p65, and IL-17 A. These alterations indicate a discernible effect of small intestinal water reabsorption, inflammatory factor levels, and smooth muscle contraction. Saccharomyces boulardii also positively regulated small intestinal metabolite levels, such as fructose 6-phosphate, dihomo-alpha-linolenic acid, and 3-(4-hydroxyphenyl) lactate, and participated in metabolic pathways such as arginine biosynthesis, linoleic acid metabolism, and protein digestion and absorption. While not fully reversing defecation changes, Saccharomyces boulardii alters intestinal flora, up-regulates key proteins affecting water reabsorption and inflammation, and positively influences metabolic pathways. Our study provides serves as a basis for further studies on the application of S. boulardii in the treatment of intestinal disorders.https://doi.org/10.1186/s12866-024-03647-0Saccharomyces boulardiiConstipationLoperamideMetaboliteKEGG pathway |
| spellingShingle | Shuai Tang Jia Li Yi Li Haitao Du Wenya Zhu Ru Zhang Jun Wan Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice BMC Microbiology Saccharomyces boulardii Constipation Loperamide Metabolite KEGG pathway |
| title | Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |
| title_full | Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |
| title_fullStr | Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |
| title_full_unstemmed | Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |
| title_short | Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |
| title_sort | effects of saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |
| topic | Saccharomyces boulardii Constipation Loperamide Metabolite KEGG pathway |
| url | https://doi.org/10.1186/s12866-024-03647-0 |
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