Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition
Objective To analyze the diversity and composition changes of gut fungal communities between mouse model of total parental nutrition (TPN) and normal control mice. Methods After mouse model of TPN was constructed, fresh feces were collected from TPN mice (n=5) and normal control mice (n=5). Interna...
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Editorial Office of Journal of Army Medical University
2024-11-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | https://aammt.tmmu.edu.cn/html/202404125.htm |
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| _version_ | 1846166061507936256 |
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| author | XIE Huichao CHEN Yihui LIU Xingyu |
| author_facet | XIE Huichao CHEN Yihui LIU Xingyu |
| author_sort | XIE Huichao |
| collection | DOAJ |
| description | Objective To analyze the diversity and composition changes of gut fungal communities between mouse model of total parental nutrition (TPN) and normal control mice. Methods After mouse model of TPN was constructed, fresh feces were collected from TPN mice (n=5) and normal control mice (n=5). Internal transcribed spacer (ITS) DNA sequencing was applied to determine intestinal fungi, and then bioinformatics analysis was conducted to identify the differences in fungal diversity, structure, and functional properties between the 2 groups of mice. Results There were significant differences in Alpha diversity (P < 0.05) and Beta diversity (P < 0.01) of intestinal fungi between the 2 groups. In the TPN model group, the relative abundances of Candida, Penicillium, Aspergillus and Talaromyces were obviously reduced (all P < 0.01). LEfSe analysis indicated that the above 4 strains were notably enriched in the normal control mice. Conclusion TPN mice exhibit characteristic changes in the composition of gut fungal flora compared to normal control mice. Dysfunction of gut fungal community may promote the occurrence of TPN related complications, and regulating the balance of gut fungal community may become a new strategy for preventing TPN related complications.
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| format | Article |
| id | doaj-art-91159e2fe958434ea9d84ff8ec1e065b |
| institution | Kabale University |
| issn | 2097-0927 |
| language | zho |
| publishDate | 2024-11-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj-art-91159e2fe958434ea9d84ff8ec1e065b2024-11-16T10:36:51ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272024-11-0146212407241410.16016/j.2097-0927.202404125Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutritionXIE Huichao0CHEN Yihui1LIU Xingyu2Department of General Surgery, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of General Surgery, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, ChinaDepartment of Nutrition, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China Objective To analyze the diversity and composition changes of gut fungal communities between mouse model of total parental nutrition (TPN) and normal control mice. Methods After mouse model of TPN was constructed, fresh feces were collected from TPN mice (n=5) and normal control mice (n=5). Internal transcribed spacer (ITS) DNA sequencing was applied to determine intestinal fungi, and then bioinformatics analysis was conducted to identify the differences in fungal diversity, structure, and functional properties between the 2 groups of mice. Results There were significant differences in Alpha diversity (P < 0.05) and Beta diversity (P < 0.01) of intestinal fungi between the 2 groups. In the TPN model group, the relative abundances of Candida, Penicillium, Aspergillus and Talaromyces were obviously reduced (all P < 0.01). LEfSe analysis indicated that the above 4 strains were notably enriched in the normal control mice. Conclusion TPN mice exhibit characteristic changes in the composition of gut fungal flora compared to normal control mice. Dysfunction of gut fungal community may promote the occurrence of TPN related complications, and regulating the balance of gut fungal community may become a new strategy for preventing TPN related complications. https://aammt.tmmu.edu.cn/html/202404125.htmtotal parental nutritionfungiits dna |
| spellingShingle | XIE Huichao CHEN Yihui LIU Xingyu Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition 陆军军医大学学报 total parental nutrition fungi its dna |
| title | Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition |
| title_full | Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition |
| title_fullStr | Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition |
| title_full_unstemmed | Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition |
| title_short | Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition |
| title_sort | diversity and composition changes of intestinal fungi in a mouse model of total parenteral nutrition |
| topic | total parental nutrition fungi its dna |
| url | https://aammt.tmmu.edu.cn/html/202404125.htm |
| work_keys_str_mv | AT xiehuichao diversityandcompositionchangesofintestinalfungiinamousemodeloftotalparenteralnutrition AT chenyihui diversityandcompositionchangesofintestinalfungiinamousemodeloftotalparenteralnutrition AT liuxingyu diversityandcompositionchangesofintestinalfungiinamousemodeloftotalparenteralnutrition |