Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs
(1) Background: This study was performed to evaluate whether the addition of β-mannanase alone or combined with a multi-carbohydrase complex can improve diet digestibility, nutrient and energy metabolism, and the gut health of growing pigs. (2) Methods: Twenty-four pigs (35.56 ± 3.81 kg) were fed a...
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2024-11-01
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| author | Gabriela Miotto Galli Ines Andretta Camila Lopes Carvalho Thais B. Stefanello Bruna Souza de Lima Cony Alícia Zem Fraga Karine Ludwig Takeuti Aline Beatriz da Rosa Marcos Kipper |
| author_facet | Gabriela Miotto Galli Ines Andretta Camila Lopes Carvalho Thais B. Stefanello Bruna Souza de Lima Cony Alícia Zem Fraga Karine Ludwig Takeuti Aline Beatriz da Rosa Marcos Kipper |
| author_sort | Gabriela Miotto Galli |
| collection | DOAJ |
| description | (1) Background: This study was performed to evaluate whether the addition of β-mannanase alone or combined with a multi-carbohydrase complex can improve diet digestibility, nutrient and energy metabolism, and the gut health of growing pigs. (2) Methods: Twenty-four pigs (35.56 ± 3.81 kg) were fed a control corn–soybean meal-based diet (no addition) or a control diet with β-mannanase (BM; 300 g/ton) or control diet β-mannanase plus a multi-carbohydrase complex including xylanase, β-glucanase, and arabinofuranosidases (BM + MCC; 300 + 50 g/ton) for 13 days. Total fecal and urine samples were collected from days 6 to 12. The feces samples were collected from all the pigs to determine fecal biomarkers using commercial ELISA tests. Blood samples were collected from all the pigs on day 13 to assess the serum concentrations of acute-phase proteins. All the pigs were euthanized on day 13 for intestinal tissue collection for morphometric analysis. Data were submitted to variance analysis and differences were considered significant at <i>p</i> ≤ 0.05 and a trend for 0.05 < <i>p</i> ≤ 0.10. (3) Results: The addition of BM and BM + MCC resulted in greater dry matter, protein, and energy digestibility coefficients, and protein (2.87% and 2.60%) and energy (2.61% and 1.44%) metabolizability coefficients compared to control (<i>p</i> < 0.05). A greater retention of nitrogen ratio and lower fecal energy were observed in BM and BM + MCC than in the control (<i>p</i> < 0.01). Furthermore, the addition of BM and BM + MCC resulted in lower manure production (29.78 and 49.77%, respectively) and fecal moisture (<i>p</i> < 0.001) compared to the control. The BM addition resulted in a greater villus area and villi height to crypt depth ratio compared to the control <i>(p</i> < 0.05). The addition of BM and BM + MCC diets also reduced the fecal calprotectin levels by 52 and 56% in relation to the control pigs. (4) Conclusions: The use of β-mannanase alone or associated with multi-carbohydrase complex improved nutritional digestibility, nutrient and energy metabolism, and gut health, and reduced the manure production of growing pigs. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-2615 |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-90ce28e4c1f44fd6b8891c744b6ff6df2024-12-13T16:21:18ZengMDPI AGAnimals2076-26152024-11-011423345710.3390/ani14233457Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing PigsGabriela Miotto Galli0Ines Andretta1Camila Lopes Carvalho2Thais B. Stefanello3Bruna Souza de Lima Cony4Alícia Zem Fraga5Karine Ludwig Takeuti6Aline Beatriz da Rosa7Marcos Kipper8Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540000, RS, BrazilFaculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540000, RS, BrazilFaculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540000, RS, BrazilFaculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540000, RS, BrazilFaculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540000, RS, BrazilFaculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540000, RS, BrazilDepartamento de Medicina Veterinária, Universidade Feevale, Novo Hamburgo 93525075, RS, BrazilDepartamento de Medicina Veterinária, Universidade Feevale, Novo Hamburgo 93525075, RS, BrazilElanco Animal Health, São Paulo 04703002, SP, Brazil(1) Background: This study was performed to evaluate whether the addition of β-mannanase alone or combined with a multi-carbohydrase complex can improve diet digestibility, nutrient and energy metabolism, and the gut health of growing pigs. (2) Methods: Twenty-four pigs (35.56 ± 3.81 kg) were fed a control corn–soybean meal-based diet (no addition) or a control diet with β-mannanase (BM; 300 g/ton) or control diet β-mannanase plus a multi-carbohydrase complex including xylanase, β-glucanase, and arabinofuranosidases (BM + MCC; 300 + 50 g/ton) for 13 days. Total fecal and urine samples were collected from days 6 to 12. The feces samples were collected from all the pigs to determine fecal biomarkers using commercial ELISA tests. Blood samples were collected from all the pigs on day 13 to assess the serum concentrations of acute-phase proteins. All the pigs were euthanized on day 13 for intestinal tissue collection for morphometric analysis. Data were submitted to variance analysis and differences were considered significant at <i>p</i> ≤ 0.05 and a trend for 0.05 < <i>p</i> ≤ 0.10. (3) Results: The addition of BM and BM + MCC resulted in greater dry matter, protein, and energy digestibility coefficients, and protein (2.87% and 2.60%) and energy (2.61% and 1.44%) metabolizability coefficients compared to control (<i>p</i> < 0.05). A greater retention of nitrogen ratio and lower fecal energy were observed in BM and BM + MCC than in the control (<i>p</i> < 0.01). Furthermore, the addition of BM and BM + MCC resulted in lower manure production (29.78 and 49.77%, respectively) and fecal moisture (<i>p</i> < 0.001) compared to the control. The BM addition resulted in a greater villus area and villi height to crypt depth ratio compared to the control <i>(p</i> < 0.05). The addition of BM and BM + MCC diets also reduced the fecal calprotectin levels by 52 and 56% in relation to the control pigs. (4) Conclusions: The use of β-mannanase alone or associated with multi-carbohydrase complex improved nutritional digestibility, nutrient and energy metabolism, and gut health, and reduced the manure production of growing pigs.https://www.mdpi.com/2076-2615/14/23/3457arabinofuranosidasesβ-glucanaseβ-mannansdigestibilityenzymesfeed additives |
| spellingShingle | Gabriela Miotto Galli Ines Andretta Camila Lopes Carvalho Thais B. Stefanello Bruna Souza de Lima Cony Alícia Zem Fraga Karine Ludwig Takeuti Aline Beatriz da Rosa Marcos Kipper Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs Animals arabinofuranosidases β-glucanase β-mannans digestibility enzymes feed additives |
| title | Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs |
| title_full | Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs |
| title_fullStr | Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs |
| title_full_unstemmed | Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs |
| title_short | Effects of β-Mannanase Alone or Combined with Multi-Carbohydrase Complex in Corn–Soybean Meal Diets on Nutrient Metabolism and Gut Health of Growing Pigs |
| title_sort | effects of β mannanase alone or combined with multi carbohydrase complex in corn soybean meal diets on nutrient metabolism and gut health of growing pigs |
| topic | arabinofuranosidases β-glucanase β-mannans digestibility enzymes feed additives |
| url | https://www.mdpi.com/2076-2615/14/23/3457 |
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