Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea

During an investigation of microfungi from various ecosystems in South Korea, two novel fungal species (KNUF-22-094T and KNUF-23-321CT) were discovered within the genera Microascus and Tolypocladium. The strain KNUF-22-094T stands out among other Microascus species due to its distinctive conidiogeno...

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Main Authors: Seong-Keun Lim, Das Kallol, Jun-Woo Choi, Min-Gyu Kim, Chang-Gi Back, In-Kyu Kang, Seung-Yeol Lee, Hee-Young Jung
Format: Article
Language:English
Published: Taylor & Francis Group 2025-05-01
Series:Mycobiology
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Online Access:https://www.tandfonline.com/doi/10.1080/12298093.2025.2474291
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author Seong-Keun Lim
Das Kallol
Jun-Woo Choi
Min-Gyu Kim
Chang-Gi Back
In-Kyu Kang
Seung-Yeol Lee
Hee-Young Jung
author_facet Seong-Keun Lim
Das Kallol
Jun-Woo Choi
Min-Gyu Kim
Chang-Gi Back
In-Kyu Kang
Seung-Yeol Lee
Hee-Young Jung
author_sort Seong-Keun Lim
collection DOAJ
description During an investigation of microfungi from various ecosystems in South Korea, two novel fungal species (KNUF-22-094T and KNUF-23-321CT) were discovered within the genera Microascus and Tolypocladium. The strain KNUF-22-094T stands out among other Microascus species due to its distinctive conidiogenous cells and conidial size ranges. The conidiogenous cells of KNUF-22-094T measured 2.3–4.3 × 2.0–3.6 µm, notably smaller than those of M. rothbergiorum (7.5–11.5 × 2.5–4.5 µm), M. sparsimycelialis (5.0–10.0 × 3.0–5.0 µm), which is considerably larger in both dimensions. For the conidial structures, conidia measured 2.6–4.8 × 2.6–4.4 µm, falling within a distinct range that is smaller than those of M. rothbergiorum (4.0–5.5 × 3.5–4.5 µm), M. verrucosus (5.0–7.0 × 4.5–6.0 µm), and M. restrictus (4.5–6.0 × 4.0–5.5 µm), but comparable in size to M. murinus, which tends to be more elongated (4.0–6.0 × 1.5–1.9 µm). The phialides of KNUF-23-321CT are globose, with a notably broad length range of 3.7–22.5 µm and a width of 0.9–1.5 µm, making it one of the most variable in length compared to species like T. album (3.5–10 µm) and T. endophyticum (1.3 ± 0.2 µm, 4.1 µm on average). Phylogenetic analyses using a multi-locus sequences supported to confirm their distinctness under the genus Microascus and Tolypocladium species. Thus, these fungal strains isolated from soil in Korea are proposed as a novel species according to their characteristics and are named Microascus microspora sp. nov. and Tolypocladium terrae sp. nov. Detailed descriptions, illustrations, and phylogenetic data are provided to support the recognition of these new species.
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spelling doaj-art-90a8f90c136b4531a72f73221c3d93672025-08-20T02:33:23ZengTaylor & Francis GroupMycobiology1229-80932092-93232025-05-0153328028810.1080/12298093.2025.2474291Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in KoreaSeong-Keun Lim0Das Kallol1Jun-Woo Choi2Min-Gyu Kim3Chang-Gi Back4In-Kyu Kang5Seung-Yeol Lee6Hee-Young Jung7Department of Plant Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Wine and Viticulture, California Polytechnic State University, San Luis Obispo, CA, USADepartment of Plant Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Plant Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Environmental Horticulture and Landscape Architecture, Dankook University, Cheonan, South KoreaDepartment of Horticultural Science, Kyungpook National University, Daegu, South KoreaDepartment of Plant Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Plant Medicine, Kyungpook National University, Daegu, South KoreaDuring an investigation of microfungi from various ecosystems in South Korea, two novel fungal species (KNUF-22-094T and KNUF-23-321CT) were discovered within the genera Microascus and Tolypocladium. The strain KNUF-22-094T stands out among other Microascus species due to its distinctive conidiogenous cells and conidial size ranges. The conidiogenous cells of KNUF-22-094T measured 2.3–4.3 × 2.0–3.6 µm, notably smaller than those of M. rothbergiorum (7.5–11.5 × 2.5–4.5 µm), M. sparsimycelialis (5.0–10.0 × 3.0–5.0 µm), which is considerably larger in both dimensions. For the conidial structures, conidia measured 2.6–4.8 × 2.6–4.4 µm, falling within a distinct range that is smaller than those of M. rothbergiorum (4.0–5.5 × 3.5–4.5 µm), M. verrucosus (5.0–7.0 × 4.5–6.0 µm), and M. restrictus (4.5–6.0 × 4.0–5.5 µm), but comparable in size to M. murinus, which tends to be more elongated (4.0–6.0 × 1.5–1.9 µm). The phialides of KNUF-23-321CT are globose, with a notably broad length range of 3.7–22.5 µm and a width of 0.9–1.5 µm, making it one of the most variable in length compared to species like T. album (3.5–10 µm) and T. endophyticum (1.3 ± 0.2 µm, 4.1 µm on average). Phylogenetic analyses using a multi-locus sequences supported to confirm their distinctness under the genus Microascus and Tolypocladium species. Thus, these fungal strains isolated from soil in Korea are proposed as a novel species according to their characteristics and are named Microascus microspora sp. nov. and Tolypocladium terrae sp. nov. Detailed descriptions, illustrations, and phylogenetic data are provided to support the recognition of these new species.https://www.tandfonline.com/doi/10.1080/12298093.2025.2474291MorphologyMicroascusphylogenyTolypocladiumsoil
spellingShingle Seong-Keun Lim
Das Kallol
Jun-Woo Choi
Min-Gyu Kim
Chang-Gi Back
In-Kyu Kang
Seung-Yeol Lee
Hee-Young Jung
Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea
Mycobiology
Morphology
Microascus
phylogeny
Tolypocladium
soil
title Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea
title_full Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea
title_fullStr Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea
title_full_unstemmed Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea
title_short Microascus microspora sp. nov. and Tolypocladium terrae sp. nov.; Two Novel Fungal Species Isolated from Soil in Korea
title_sort microascus microspora sp nov and tolypocladium terrae sp nov two novel fungal species isolated from soil in korea
topic Morphology
Microascus
phylogeny
Tolypocladium
soil
url https://www.tandfonline.com/doi/10.1080/12298093.2025.2474291
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