Serological surveillance of noroviruses in a community-based prospective cohort: a study protocol
Introduction Noroviruses are the leading cause of viral acute gastroenteritis affecting all age groups. Since 2014, the previous rarely reported GII.P17-GII.17 and recombinant GII.P16-GII.2 norovirus emerged, replacing GII.4 predominant genotype, causing increased outbreaks in China and other countr...
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BMJ Publishing Group
2021-03-01
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| Series: | BMJ Open |
| Online Access: | https://bmjopen.bmj.com/content/11/3/e043228.full |
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| author | Lu Wang Dongjie Xie Jingrong Yu Mark Momoh Koroma Mengsi Qiu Wentao Duan Xu-Fu Zhang Ying-Chun Dai |
| author_facet | Lu Wang Dongjie Xie Jingrong Yu Mark Momoh Koroma Mengsi Qiu Wentao Duan Xu-Fu Zhang Ying-Chun Dai |
| author_sort | Lu Wang |
| collection | DOAJ |
| description | Introduction Noroviruses are the leading cause of viral acute gastroenteritis affecting all age groups. Since 2014, the previous rarely reported GII.P17-GII.17 and recombinant GII.P16-GII.2 norovirus emerged, replacing GII.4 predominant genotype, causing increased outbreaks in China and other countries. Meanwhile, GII.4/2012 Sydney strain has re-emerged as the dominant variant in many places in 2015–2018. The role of herd immunity as the driving force during these new emerging or re-emerging noroviruses is poorly defined. Serological surveillance studies on community-based prospective cohort on norovirus are highly needed.Methods and analyses This study will include 1000 out of 9798 participants aged 18 years and above from Caofeidian district, Tangshan city, northern China. Baseline data on sociodemographic characteristics and blood samples were collected in 2013–2014. Blood collection will be replicated annually throughout the cohort until 2023. Saliva samples were also collected in 2016. The seroprevalence and seroincidence of blockade antibodies against norovirus genotypes of GII.P17-GII.17, GII.P16-GII.2, the re-emerged GII.4/2012 and potential novel pandemic variants will be evaluated by ELISA. Associations between genotype blockade antibodies and sociodemographic factors and human histo-blood group antigens will be evaluated using univariate and multivariate analysis. The dynamics of herd immunity duration will be estimated in this longitudinal surveillance.Ethics and dissemination The study has been approved by the Ethical Committees of the Staff Hospital of Jidong oil-field of China National Petroleum Corporation. This study will provide insight into the seroprevalence and seroincidence of noroviruses, and their relationships with sociodemographic characteristics and genetic susceptibility. It will also explain herd immunity of the emerged and re-emerged genotypes or variants. The study will further enable an understanding of the mechanism driving the replacement of norovirus genotypes. Research findings will be disseminated in peer-reviewed journals and at scientific meetings. |
| format | Article |
| id | doaj-art-90725ec334884dbe91f49dda2fc643b0 |
| institution | Kabale University |
| issn | 2044-6055 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Open |
| spelling | doaj-art-90725ec334884dbe91f49dda2fc643b02024-11-17T22:15:08ZengBMJ Publishing GroupBMJ Open2044-60552021-03-0111310.1136/bmjopen-2020-043228Serological surveillance of noroviruses in a community-based prospective cohort: a study protocolLu Wang0Dongjie Xie1Jingrong Yu2Mark Momoh Koroma3Mengsi Qiu4Wentao Duan5Xu-Fu Zhang6Ying-Chun Dai74 Department of Acupuncture and Rehabilitation, Hospital of Chengdu Universily of TCM, Chengdu, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Epidemiology,Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong, China4 Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Epidemiology,Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Epidemiology,Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong, ChinaIntroduction Noroviruses are the leading cause of viral acute gastroenteritis affecting all age groups. Since 2014, the previous rarely reported GII.P17-GII.17 and recombinant GII.P16-GII.2 norovirus emerged, replacing GII.4 predominant genotype, causing increased outbreaks in China and other countries. Meanwhile, GII.4/2012 Sydney strain has re-emerged as the dominant variant in many places in 2015–2018. The role of herd immunity as the driving force during these new emerging or re-emerging noroviruses is poorly defined. Serological surveillance studies on community-based prospective cohort on norovirus are highly needed.Methods and analyses This study will include 1000 out of 9798 participants aged 18 years and above from Caofeidian district, Tangshan city, northern China. Baseline data on sociodemographic characteristics and blood samples were collected in 2013–2014. Blood collection will be replicated annually throughout the cohort until 2023. Saliva samples were also collected in 2016. The seroprevalence and seroincidence of blockade antibodies against norovirus genotypes of GII.P17-GII.17, GII.P16-GII.2, the re-emerged GII.4/2012 and potential novel pandemic variants will be evaluated by ELISA. Associations between genotype blockade antibodies and sociodemographic factors and human histo-blood group antigens will be evaluated using univariate and multivariate analysis. The dynamics of herd immunity duration will be estimated in this longitudinal surveillance.Ethics and dissemination The study has been approved by the Ethical Committees of the Staff Hospital of Jidong oil-field of China National Petroleum Corporation. This study will provide insight into the seroprevalence and seroincidence of noroviruses, and their relationships with sociodemographic characteristics and genetic susceptibility. It will also explain herd immunity of the emerged and re-emerged genotypes or variants. The study will further enable an understanding of the mechanism driving the replacement of norovirus genotypes. Research findings will be disseminated in peer-reviewed journals and at scientific meetings.https://bmjopen.bmj.com/content/11/3/e043228.full |
| spellingShingle | Lu Wang Dongjie Xie Jingrong Yu Mark Momoh Koroma Mengsi Qiu Wentao Duan Xu-Fu Zhang Ying-Chun Dai Serological surveillance of noroviruses in a community-based prospective cohort: a study protocol BMJ Open |
| title | Serological surveillance of noroviruses in a community-based prospective cohort: a study protocol |
| title_full | Serological surveillance of noroviruses in a community-based prospective cohort: a study protocol |
| title_fullStr | Serological surveillance of noroviruses in a community-based prospective cohort: a study protocol |
| title_full_unstemmed | Serological surveillance of noroviruses in a community-based prospective cohort: a study protocol |
| title_short | Serological surveillance of noroviruses in a community-based prospective cohort: a study protocol |
| title_sort | serological surveillance of noroviruses in a community based prospective cohort a study protocol |
| url | https://bmjopen.bmj.com/content/11/3/e043228.full |
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