Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for microbiome studies

Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for microbiome studies

Abstract Most respiratory microbiome studies use amplicon sequencing due to high host DNA. Metagenomics sequencing offers finer taxonomic resolution, phage assessment, and functional characterization. We evaluated five host DNA depletion methods on frozen nasal swabs from healthy adults, sputum from...

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Main Authors: Minsik Kim, Raymond C. Parrish, Michael J. Tisza, Viral S. Shah, Thi Tran, Matthew Ross, Juwan Cormier, Aribah Baig, Ching-Ying Huang, Laura Brenner, Isabel Neuringer, Katrine Whiteson, J. Kirk Harris, Amy D. Willis, Peggy S. Lai
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-024-07290-3
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