Establishment of a rapid method for the detection of Brucella canis based on recombinase-mediated thermostable nucleic acid amplification technology

Establishment of a rapid method for the detection of Brucella canis based on recombinase-mediated thermostable nucleic acid amplification technology

ObjectiveTo establish a rapid detection method for canine brucellosis using recombinase-aided amplification (RAA) technology.MethodsThe outer membrane protein 25 gene fragment (Omp25) of Brucella canis was targeted. Primers and fluorescent probes were designed and synthesized, and recombinant plasmi...

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Bibliographic Details
Main Authors:	Shao-Zheng Song, Zi-Yuan Li, Yuan-Yuan Liu, Ying-Chao Wu, Kang-Ying Yu, Zhengyi He
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-01-01
Series:	Frontiers in Cellular and Infection Microbiology
Subjects:	recombinase thermostatic nucleic acid amplification rapid detection Brucella canis
Online Access:	https://www.frontiersin.org/articles/10.3389/fcimb.2024.1493492/full
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