Cloning and functional analysis of ZmMADS42 gene in maize
Maize (Zea mays L.) is the most important cereal crop in the world. Flowering period and photoperiod play important roles in the reproductive development of maize. This study, investigated ZmMADS42, a gene that is highly expressed in the shoot apical meristem. Agrobacterium infection was used to suc...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | GM Crops & Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21645698.2024.2328384 |
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| author | Yang Zhao Jianyu Lu Bo Hu Peng Jiao Bai Gao Zhenzhong Jiang Siyan Liu Shuyan Guan Yiyong Ma |
| author_facet | Yang Zhao Jianyu Lu Bo Hu Peng Jiao Bai Gao Zhenzhong Jiang Siyan Liu Shuyan Guan Yiyong Ma |
| author_sort | Yang Zhao |
| collection | DOAJ |
| description | Maize (Zea mays L.) is the most important cereal crop in the world. Flowering period and photoperiod play important roles in the reproductive development of maize. This study, investigated ZmMADS42, a gene that is highly expressed in the shoot apical meristem. Agrobacterium infection was used to successfully obtain overexpressed ZmMADS42 plants. Fluorescence quantitative PCR revealed that the expression of the ZmMADS42 gene in the shoot apical meristem of transgenic plants was 2.8 times higher than that of the wild-type(WT). In addition, the expression of the ZmMADS42 gene in the endosperm was 2.4 times higher than that in the wild-type. The seed width of the T2 generation increased by 5.35%, whereas the seed length decreased by 7.78% compared with that of the wild-type. Dissection of the shoot tips of transgenic and wild-type plants from the 7-leaf stage to the 9-leaf stage revealed that the transgenic plants entered the differentiation stage earlier and exhibited more tassel meristems during their vegetative growth period. The mature transgenic plants were approximately 20 cm shorter in height and had a lower panicle position than the wild-type plants. Comparing the flowering period, the tasseling, powdering, and silking stages of the transgenic plants occurred 10 days earlier than those of the wild-type plants. The results showed that the ZmMADS42 gene played a significant role in regulating the flowering period and plant height of maize. |
| format | Article |
| id | doaj-art-902493b38fbd4f29ba14a795bc242130 |
| institution | Kabale University |
| issn | 2164-5698 2164-5701 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | GM Crops & Food |
| spelling | doaj-art-902493b38fbd4f29ba14a795bc2421302024-12-16T16:41:27ZengTaylor & Francis GroupGM Crops & Food2164-56982164-57012024-12-0115110511710.1080/21645698.2024.2328384Cloning and functional analysis of ZmMADS42 gene in maizeYang ZhaoJianyu Lu0Bo Hu1Peng JiaoBai Gao2Zhenzhong Jiang3Siyan LiuShuyan Guan4Yiyong Ma5College of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaMaize (Zea mays L.) is the most important cereal crop in the world. Flowering period and photoperiod play important roles in the reproductive development of maize. This study, investigated ZmMADS42, a gene that is highly expressed in the shoot apical meristem. Agrobacterium infection was used to successfully obtain overexpressed ZmMADS42 plants. Fluorescence quantitative PCR revealed that the expression of the ZmMADS42 gene in the shoot apical meristem of transgenic plants was 2.8 times higher than that of the wild-type(WT). In addition, the expression of the ZmMADS42 gene in the endosperm was 2.4 times higher than that in the wild-type. The seed width of the T2 generation increased by 5.35%, whereas the seed length decreased by 7.78% compared with that of the wild-type. Dissection of the shoot tips of transgenic and wild-type plants from the 7-leaf stage to the 9-leaf stage revealed that the transgenic plants entered the differentiation stage earlier and exhibited more tassel meristems during their vegetative growth period. The mature transgenic plants were approximately 20 cm shorter in height and had a lower panicle position than the wild-type plants. Comparing the flowering period, the tasseling, powdering, and silking stages of the transgenic plants occurred 10 days earlier than those of the wild-type plants. The results showed that the ZmMADS42 gene played a significant role in regulating the flowering period and plant height of maize.https://www.tandfonline.com/doi/10.1080/21645698.2024.2328384Flowering periodMADS-boxmaizeplant heightZmMADS42 |
| spellingShingle | Yang Zhao Jianyu Lu Bo Hu Peng Jiao Bai Gao Zhenzhong Jiang Siyan Liu Shuyan Guan Yiyong Ma Cloning and functional analysis of ZmMADS42 gene in maize GM Crops & Food Flowering period MADS-box maize plant height ZmMADS42 |
| title | Cloning and functional analysis of ZmMADS42 gene in maize |
| title_full | Cloning and functional analysis of ZmMADS42 gene in maize |
| title_fullStr | Cloning and functional analysis of ZmMADS42 gene in maize |
| title_full_unstemmed | Cloning and functional analysis of ZmMADS42 gene in maize |
| title_short | Cloning and functional analysis of ZmMADS42 gene in maize |
| title_sort | cloning and functional analysis of zmmads42 gene in maize |
| topic | Flowering period MADS-box maize plant height ZmMADS42 |
| url | https://www.tandfonline.com/doi/10.1080/21645698.2024.2328384 |
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