Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon

This study aimed to assess the behaviour of phenolic compounds from yarrow extract encapsulated in nanoemulsions during in vitro gastrointestinal digestion. Oil-in-water nanoemulsions were developed using grape seed oil and lupin protein (LPI) as oil phase and emulsifier, respectively. The use of 6...

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Main Authors: María de las Nieves Siles-Sánchez, Laura Jaime, Milena Corredig, Susana Santoyo, Elena Arranz
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Food Hydrocolloids for Health
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2667025924000116
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author María de las Nieves Siles-Sánchez
Laura Jaime
Milena Corredig
Susana Santoyo
Elena Arranz
author_facet María de las Nieves Siles-Sánchez
Laura Jaime
Milena Corredig
Susana Santoyo
Elena Arranz
author_sort María de las Nieves Siles-Sánchez
collection DOAJ
description This study aimed to assess the behaviour of phenolic compounds from yarrow extract encapsulated in nanoemulsions during in vitro gastrointestinal digestion. Oil-in-water nanoemulsions were developed using grape seed oil and lupin protein (LPI) as oil phase and emulsifier, respectively. The use of 6 % LPI including 1 mg/mL of yarrow extract resulted in nanoemulsions with a homogeneous particle size distribution (200 nm) and an encapsulation efficiency of 85.6 %. During in vitro gastrointestinal digestion, most of the phenolics remained encapsulated, being protected from degradation. The in vitro bioavailability of the encapsulated phenolics was measured using a cell co-culture model (Caco-2/HT-29MTX). In this regard, nanoemulsions did not increase the bioavailability of yarrow phenolics, instead, they promoted their access to the colon. Finally, the antiproliferative activity was determined in Caco-2 cells, observing that the apical fraction inhibited cancer cells, indicating the bioefficacy of the non-absorbed phenolics. Thus, this study underscores the potential of LPI-stabilized nanoemulsions as a vehicle for protecting and delivering yarrow phenolics to the colon.
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institution Kabale University
issn 2667-0259
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publishDate 2024-12-01
publisher Elsevier
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series Food Hydrocolloids for Health
spelling doaj-art-9016f2fb914a4f56bc120ff9e3085a0d2024-12-17T05:01:39ZengElsevierFood Hydrocolloids for Health2667-02592024-12-016100186Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colonMaría de las Nieves Siles-Sánchez0Laura Jaime1Milena Corredig2Susana Santoyo3Elena Arranz4Universidad Autónoma de Madrid (CEI UAM+CSIC), Institute of Food Science and Research (CIAL), 28049 Madrid, Spain; Department of Food Science, CiFood Center for Innovative Foods, Aarhus University, 8200 Aarhus, Denmark; Corresponding author.Universidad Autónoma de Madrid (CEI UAM+CSIC), Institute of Food Science and Research (CIAL), 28049 Madrid, SpainDepartment of Food Science, CiFood Center for Innovative Foods, Aarhus University, 8200 Aarhus, DenmarkUniversidad Autónoma de Madrid (CEI UAM+CSIC), Institute of Food Science and Research (CIAL), 28049 Madrid, SpainUniversidad Autónoma de Madrid (CEI UAM+CSIC), Institute of Food Science and Research (CIAL), 28049 Madrid, SpainThis study aimed to assess the behaviour of phenolic compounds from yarrow extract encapsulated in nanoemulsions during in vitro gastrointestinal digestion. Oil-in-water nanoemulsions were developed using grape seed oil and lupin protein (LPI) as oil phase and emulsifier, respectively. The use of 6 % LPI including 1 mg/mL of yarrow extract resulted in nanoemulsions with a homogeneous particle size distribution (200 nm) and an encapsulation efficiency of 85.6 %. During in vitro gastrointestinal digestion, most of the phenolics remained encapsulated, being protected from degradation. The in vitro bioavailability of the encapsulated phenolics was measured using a cell co-culture model (Caco-2/HT-29MTX). In this regard, nanoemulsions did not increase the bioavailability of yarrow phenolics, instead, they promoted their access to the colon. Finally, the antiproliferative activity was determined in Caco-2 cells, observing that the apical fraction inhibited cancer cells, indicating the bioefficacy of the non-absorbed phenolics. Thus, this study underscores the potential of LPI-stabilized nanoemulsions as a vehicle for protecting and delivering yarrow phenolics to the colon.http://www.sciencedirect.com/science/article/pii/S2667025924000116YarrowPhenolic compoundsLupin proteinNanoemulsionsDelivery system
spellingShingle María de las Nieves Siles-Sánchez
Laura Jaime
Milena Corredig
Susana Santoyo
Elena Arranz
Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon
Food Hydrocolloids for Health
Yarrow
Phenolic compounds
Lupin protein
Nanoemulsions
Delivery system
title Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon
title_full Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon
title_fullStr Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon
title_full_unstemmed Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon
title_short Encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon
title_sort encapsulation of yarrow phenolic compounds in lupin protein nanoemulsions increases stability during gastrointestinal transit and delivery in the colon
topic Yarrow
Phenolic compounds
Lupin protein
Nanoemulsions
Delivery system
url http://www.sciencedirect.com/science/article/pii/S2667025924000116
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