LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesia
Knowledge of drug pharmacokinetics and tissue distribution is precious for ensuring patient safety and optimizing treatments. The varied use of local anesthetics, as well as the fact that anesthetics can sometimes have low therapeutic indices and numerous adverse drug reactions, makes any novel phar...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1494646/full |
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| author | Mihaela Butiulca Mihaela Butiulca Lenard Farczadi Silvia Imre Silvia Imre Camil Eugen Vari Laurian Vlase Bogdan Cordos Leonard Azamfirei Leonard Azamfirei Alexandra Elena Lazar Alexandra Elena Lazar |
| author_facet | Mihaela Butiulca Mihaela Butiulca Lenard Farczadi Silvia Imre Silvia Imre Camil Eugen Vari Laurian Vlase Bogdan Cordos Leonard Azamfirei Leonard Azamfirei Alexandra Elena Lazar Alexandra Elena Lazar |
| author_sort | Mihaela Butiulca |
| collection | DOAJ |
| description | Knowledge of drug pharmacokinetics and tissue distribution is precious for ensuring patient safety and optimizing treatments. The varied use of local anesthetics, as well as the fact that anesthetics can sometimes have low therapeutic indices and numerous adverse drug reactions, makes any novel pharmacokinetics information valuable. The present manuscript describes a pharmacokinetic study of ropivacaine carried out after plane block anesthesia on an animal model, using high sensitivity, accurate, and precise LC-MS/MS bioanalysis. Both plasmatic concentrations and tissue distribution of ropivacaine and its primary active metabolite were determined. The results showed a tissue affinity of the anesthetics, a clearance of ropivacaine mainly by hepatic metabolism, and the final, mainly renal excretion of the hydroxylated metabolite. While the results cannot simply and directly be transposed to human pharmacokinetics, they offer a valuable basis for future studies and can contribute to a better understanding of the bioavailability and toxicology of the widely used modern anesthetic. |
| format | Article |
| id | doaj-art-8f9e67fad4084e389a8cc76ecf2889e8 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-8f9e67fad4084e389a8cc76ecf2889e82025-01-07T05:23:57ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.14946461494646LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesiaMihaela Butiulca0Mihaela Butiulca1Lenard Farczadi2Silvia Imre3Silvia Imre4Camil Eugen Vari5Laurian Vlase6Bogdan Cordos7Leonard Azamfirei8Leonard Azamfirei9Alexandra Elena Lazar10Alexandra Elena Lazar11Department of Anaesthesiology and Intensive Care Medicine, Faculty of General Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaDepartment of Anaesthesiology and Intensive Care Medicine, Emergency County Hospital, Târgu Mureș, RomaniaChromatography and Mass Spectrometry Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaChromatography and Mass Spectrometry Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaDepartment of Analytical Chemistry and Drug Analysis, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaDepartment of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaDepartment of Pharmaceutic Technology and Biopharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Experimental and Imaging Studies, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaDepartment of Anaesthesiology and Intensive Care Medicine, Faculty of General Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaDepartment of Anaesthesiology and Intensive Care Medicine, Emergency County Hospital, Târgu Mureș, RomaniaDepartment of Anaesthesiology and Intensive Care Medicine, Faculty of General Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Târgu Mureș, RomaniaDepartment of Anaesthesiology and Intensive Care Medicine, Emergency County Hospital, Târgu Mureș, RomaniaKnowledge of drug pharmacokinetics and tissue distribution is precious for ensuring patient safety and optimizing treatments. The varied use of local anesthetics, as well as the fact that anesthetics can sometimes have low therapeutic indices and numerous adverse drug reactions, makes any novel pharmacokinetics information valuable. The present manuscript describes a pharmacokinetic study of ropivacaine carried out after plane block anesthesia on an animal model, using high sensitivity, accurate, and precise LC-MS/MS bioanalysis. Both plasmatic concentrations and tissue distribution of ropivacaine and its primary active metabolite were determined. The results showed a tissue affinity of the anesthetics, a clearance of ropivacaine mainly by hepatic metabolism, and the final, mainly renal excretion of the hydroxylated metabolite. While the results cannot simply and directly be transposed to human pharmacokinetics, they offer a valuable basis for future studies and can contribute to a better understanding of the bioavailability and toxicology of the widely used modern anesthetic.https://www.frontiersin.org/articles/10.3389/fphar.2024.1494646/fullropivacainepharmacokineticsLC-MS/MSratsHPLC |
| spellingShingle | Mihaela Butiulca Mihaela Butiulca Lenard Farczadi Silvia Imre Silvia Imre Camil Eugen Vari Laurian Vlase Bogdan Cordos Leonard Azamfirei Leonard Azamfirei Alexandra Elena Lazar Alexandra Elena Lazar LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesia Frontiers in Pharmacology ropivacaine pharmacokinetics LC-MS/MS rats HPLC |
| title | LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesia |
| title_full | LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesia |
| title_fullStr | LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesia |
| title_full_unstemmed | LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesia |
| title_short | LC-MS/MS assisted pharmacokinetic and tissue distribution study of ropivacaine and 3-OH-ropivacaine on rats after plane block anesthesia |
| title_sort | lc ms ms assisted pharmacokinetic and tissue distribution study of ropivacaine and 3 oh ropivacaine on rats after plane block anesthesia |
| topic | ropivacaine pharmacokinetics LC-MS/MS rats HPLC |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1494646/full |
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