Respiratory tract bacteria distribution and transmission patterns among individuals in close contact
Abstract Background Respiratory disease outbreaks frequently occur in settings where individuals are in close contact, for example, schools and factories. However, the transmission patterns of oropharyngeal microbiota among healthy individuals living in clusters are unclear. Therefore, we aimed to i...
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BMC
2024-11-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-024-10019-6 |
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| author | Na Zhao Xingxing Lian Juan Du Hongyu Ren Tianshuo Zhao Qingbin Lu Yinan Li Fuqiang Cui Tian Qin |
| author_facet | Na Zhao Xingxing Lian Juan Du Hongyu Ren Tianshuo Zhao Qingbin Lu Yinan Li Fuqiang Cui Tian Qin |
| author_sort | Na Zhao |
| collection | DOAJ |
| description | Abstract Background Respiratory disease outbreaks frequently occur in settings where individuals are in close contact, for example, schools and factories. However, the transmission patterns of oropharyngeal microbiota among healthy individuals living in clusters are unclear. Therefore, we aimed to investigate the respiratory tract bacteria distribution and transmission patterns among individuals in close contact. Methods A total of 36 freshmen from Peking University Medical School participated in the study. We collected pharyngeal swabs on the first day of enrollment, 15, 30, and 60 days after cohabitation. DNA was extracted from the swabs and subjected to high-throughput sequencing to profile the microbial composition. Statistical analyses were performed to assess diversity and significance. Results Neisseriaceae, Prevotellaceae, and Streptococcaceae were the most abundant bacterial families detected. Over time, changes were observed in the bacterial communities, with a tendency for increased similarity between dormitory room members. By day 60 of cohabitation, the bacterial communities appeared to be more similar compared to the baseline (prior to cohabitation). The transmission patterns included spreading with colonization, spreading without colonization, and non-spreading. Bacteria belonging to the core genera are most likely to spread and colonize easily. Conclusion The risk of healthy cohabitants acquiring respiratory pathogens through close contact may be overestimated in epidemiological studies. Therefore, monitoring the spread of core genera that are easily transmitted and colonized is crucial for effective prevention of respiratory pathogen transmission. |
| format | Article |
| id | doaj-art-8f8233c9cdd64092bdfed319430fb8be |
| institution | Kabale University |
| issn | 1471-2334 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj-art-8f8233c9cdd64092bdfed319430fb8be2024-11-17T12:12:04ZengBMCBMC Infectious Diseases1471-23342024-11-012411910.1186/s12879-024-10019-6Respiratory tract bacteria distribution and transmission patterns among individuals in close contactNa Zhao0Xingxing Lian1Juan Du2Hongyu Ren3Tianshuo Zhao4Qingbin Lu5Yinan Li6Fuqiang Cui7Tian Qin8National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionDepartment of Laboratorial Science and Technology & Vaccine Research Center, School of Public Health, Peking UniversityNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionDepartment of Laboratorial Science and Technology & Vaccine Research Center, School of Public Health, Peking UniversityDepartment of Laboratorial Science and Technology & Vaccine Research Center, School of Public Health, Peking UniversityNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionDepartment of Laboratorial Science and Technology & Vaccine Research Center, School of Public Health, Peking UniversityNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and PreventionAbstract Background Respiratory disease outbreaks frequently occur in settings where individuals are in close contact, for example, schools and factories. However, the transmission patterns of oropharyngeal microbiota among healthy individuals living in clusters are unclear. Therefore, we aimed to investigate the respiratory tract bacteria distribution and transmission patterns among individuals in close contact. Methods A total of 36 freshmen from Peking University Medical School participated in the study. We collected pharyngeal swabs on the first day of enrollment, 15, 30, and 60 days after cohabitation. DNA was extracted from the swabs and subjected to high-throughput sequencing to profile the microbial composition. Statistical analyses were performed to assess diversity and significance. Results Neisseriaceae, Prevotellaceae, and Streptococcaceae were the most abundant bacterial families detected. Over time, changes were observed in the bacterial communities, with a tendency for increased similarity between dormitory room members. By day 60 of cohabitation, the bacterial communities appeared to be more similar compared to the baseline (prior to cohabitation). The transmission patterns included spreading with colonization, spreading without colonization, and non-spreading. Bacteria belonging to the core genera are most likely to spread and colonize easily. Conclusion The risk of healthy cohabitants acquiring respiratory pathogens through close contact may be overestimated in epidemiological studies. Therefore, monitoring the spread of core genera that are easily transmitted and colonized is crucial for effective prevention of respiratory pathogen transmission.https://doi.org/10.1186/s12879-024-10019-6Respiratory diseaseClose contactOropharyngeal microbiotaHigh-throughput sequencing |
| spellingShingle | Na Zhao Xingxing Lian Juan Du Hongyu Ren Tianshuo Zhao Qingbin Lu Yinan Li Fuqiang Cui Tian Qin Respiratory tract bacteria distribution and transmission patterns among individuals in close contact BMC Infectious Diseases Respiratory disease Close contact Oropharyngeal microbiota High-throughput sequencing |
| title | Respiratory tract bacteria distribution and transmission patterns among individuals in close contact |
| title_full | Respiratory tract bacteria distribution and transmission patterns among individuals in close contact |
| title_fullStr | Respiratory tract bacteria distribution and transmission patterns among individuals in close contact |
| title_full_unstemmed | Respiratory tract bacteria distribution and transmission patterns among individuals in close contact |
| title_short | Respiratory tract bacteria distribution and transmission patterns among individuals in close contact |
| title_sort | respiratory tract bacteria distribution and transmission patterns among individuals in close contact |
| topic | Respiratory disease Close contact Oropharyngeal microbiota High-throughput sequencing |
| url | https://doi.org/10.1186/s12879-024-10019-6 |
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