Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana
Abortion is one of the major causes of economic losses in livestock production worldwide. Because several factors can lead to abortion in cattle, sheep and goats, laboratory diagnosis, including the molecular detection of pathogens causing abortion, is often necessary. Bacterial zoonotic diseases su...
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2024-12-01
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| author | Boitumelo M. Modise-Tlotleng Sununguko W. Mpoloka Tirumala B. K. Settypalli Joseph Hyera Tebogo Kgotlele Kago Kumile Mosarwa E. Sechele Obuile O. Raboloko Chandapiwa Marobela-Raborokgwe Gerrit J. Viljoen Giovanni Cattoli Charles E. Lamien |
| author_facet | Boitumelo M. Modise-Tlotleng Sununguko W. Mpoloka Tirumala B. K. Settypalli Joseph Hyera Tebogo Kgotlele Kago Kumile Mosarwa E. Sechele Obuile O. Raboloko Chandapiwa Marobela-Raborokgwe Gerrit J. Viljoen Giovanni Cattoli Charles E. Lamien |
| author_sort | Boitumelo M. Modise-Tlotleng |
| collection | DOAJ |
| description | Abortion is one of the major causes of economic losses in livestock production worldwide. Because several factors can lead to abortion in cattle, sheep and goats, laboratory diagnosis, including the molecular detection of pathogens causing abortion, is often necessary. Bacterial zoonotic diseases such as brucellosis, coxiellosis, leptospirosis, and listeriosis have been implicated in livestock abortion, but they are under diagnosed and under-reported in most developing countries, including Botswana. This study applied a recently developed multiplex high-resolution melting analysis technique, coupled with singleplex qPCR assays, to investigate abortions in livestock in Botswana, using 152 samples from cattle, sheep, and goat abortion cases. <i>Brucella</i> spp. were the most frequent pathogen detected, with an overall frequency of 21.1%, followed by <i>Coxiella burnetii</i> with 19.1%. <i>Listeria monocytogenes</i> and <i>Leptospira</i> spp. were not detected in any of specimens samples investigated. Mixed infections with <i>Brucella</i> spp. and <i>C. burnetii</i> were observed in 35% specimes examined. There was a good agreement between the multiplex qPCR-HRM and singleplex qPCR for detecting <i>Brucella</i> spp. and <i>C. burnetii</i>. This study is the first report on the syndromic testing of abortion-causing pathogens in Botswana. It shows the importance of molecular methods in the differential diagnosis of abortion-causing diseases in domestic ruminants. |
| format | Article |
| id | doaj-art-8f4eddc421cf45ec8367b66d2c7951f1 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-8f4eddc421cf45ec8367b66d2c7951f12024-12-27T14:41:50ZengMDPI AGMicroorganisms2076-26072024-12-011212264410.3390/microorganisms12122644Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in BotswanaBoitumelo M. Modise-Tlotleng0Sununguko W. Mpoloka1Tirumala B. K. Settypalli2Joseph Hyera3Tebogo Kgotlele4Kago Kumile5Mosarwa E. Sechele6Obuile O. Raboloko7Chandapiwa Marobela-Raborokgwe8Gerrit J. Viljoen9Giovanni Cattoli10Charles E. Lamien11National Agricultural Research and Development Institute, Private Bag 0035, Gaborone, BotswanaDepartment of Biological Sciences, University of Botswana, Private Bag 00704, Gaborone, BotswanaAnimal Production and Health Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400 Vienna, AustriaBotswana Vaccine Institute, Private Bag 0031, Gaborone, BotswanaNational Agricultural Research and Development Institute, Private Bag 0035, Gaborone, BotswanaNational Agricultural Research and Development Institute, Private Bag 0035, Gaborone, BotswanaDepartment of Veterinary Services (DVS), Ministry of Agriculture, Private Bag 0032, Gaborone, BotswanaDepartment of Veterinary Services (DVS), Ministry of Agriculture, Private Bag 0032, Gaborone, BotswanaDepartment of Veterinary Services (DVS), Ministry of Agriculture, Private Bag 0032, Gaborone, BotswanaAnimal Production and Health Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400 Vienna, AustriaIstituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Università 10, 35020 Legnaro, ItalyAnimal Production and Health Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400 Vienna, AustriaAbortion is one of the major causes of economic losses in livestock production worldwide. Because several factors can lead to abortion in cattle, sheep and goats, laboratory diagnosis, including the molecular detection of pathogens causing abortion, is often necessary. Bacterial zoonotic diseases such as brucellosis, coxiellosis, leptospirosis, and listeriosis have been implicated in livestock abortion, but they are under diagnosed and under-reported in most developing countries, including Botswana. This study applied a recently developed multiplex high-resolution melting analysis technique, coupled with singleplex qPCR assays, to investigate abortions in livestock in Botswana, using 152 samples from cattle, sheep, and goat abortion cases. <i>Brucella</i> spp. were the most frequent pathogen detected, with an overall frequency of 21.1%, followed by <i>Coxiella burnetii</i> with 19.1%. <i>Listeria monocytogenes</i> and <i>Leptospira</i> spp. were not detected in any of specimens samples investigated. Mixed infections with <i>Brucella</i> spp. and <i>C. burnetii</i> were observed in 35% specimes examined. There was a good agreement between the multiplex qPCR-HRM and singleplex qPCR for detecting <i>Brucella</i> spp. and <i>C. burnetii</i>. This study is the first report on the syndromic testing of abortion-causing pathogens in Botswana. It shows the importance of molecular methods in the differential diagnosis of abortion-causing diseases in domestic ruminants.https://www.mdpi.com/2076-2607/12/12/2644brucellosisleptospirosiscoxiellosislisteriosissyndromic surveillancezoonotic |
| spellingShingle | Boitumelo M. Modise-Tlotleng Sununguko W. Mpoloka Tirumala B. K. Settypalli Joseph Hyera Tebogo Kgotlele Kago Kumile Mosarwa E. Sechele Obuile O. Raboloko Chandapiwa Marobela-Raborokgwe Gerrit J. Viljoen Giovanni Cattoli Charles E. Lamien Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana Microorganisms brucellosis leptospirosis coxiellosis listeriosis syndromic surveillance zoonotic |
| title | Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana |
| title_full | Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana |
| title_fullStr | Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana |
| title_full_unstemmed | Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana |
| title_short | Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana |
| title_sort | molecular testing of zoonotic bacteria in cattle sheep and goat abortion cases in botswana |
| topic | brucellosis leptospirosis coxiellosis listeriosis syndromic surveillance zoonotic |
| url | https://www.mdpi.com/2076-2607/12/12/2644 |
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