Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids
Chitosan intercalated montmorillonite and polyvinyl alcohol-citric acid (PVA/CA) nanofibers were electrospun onto stainless steel wires. The fibers were placed inside a stainless steel tube and utilized for fiber-in-tube solid phase microextraction (FIT-SPME) to detect trace levels of tricyclic anti...
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Elsevier
2025-02-01
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| Series: | Advances in Sample Preparation |
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| author | Saeed Pourhosseini Yadollah Yamini Seyedeh Sara Nasrollahi |
| author_facet | Saeed Pourhosseini Yadollah Yamini Seyedeh Sara Nasrollahi |
| author_sort | Saeed Pourhosseini |
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| description | Chitosan intercalated montmorillonite and polyvinyl alcohol-citric acid (PVA/CA) nanofibers were electrospun onto stainless steel wires. The fibers were placed inside a stainless steel tube and utilized for fiber-in-tube solid phase microextraction (FIT-SPME) to detect trace levels of tricyclic antidepressant drugs (TCAs) in biological samples. The analytes were separated and quantified using gas chromatography coupled with mass spectrometric detection (GC–MS). The effects of various parameters on the extraction efficiency, including pH (9), extraction time (25 min), extraction flow rate (3 mL min-1), salt addition (0 % w/v), type of eluent (ethanol 99 %), eluent volume (300 µL), and desorption flow rate (70 µL min-1), were investigated and optimized. Under optimal conditions, the linear dynamic range for doxepin, and sertraline was found to be 0.08–750.0 ng mL-1; for amitriptyline, desipramine, and trimipramine, it was 10.0–750.0 ng mL-1; and for carbamazepine and maprotiline, it was 50.0–750.0 ng mL-1. The proposed method exhibited low limits of detections in the range of 0.02–15.2 ng mL-1. Additionally, coefficients of determination (R²) exceeding 0.9911 were achieved, indicating excellent linearity for the calibration curves. To assess the feasibility of the FIT-SPME-GC–MS method, extraction and analysis of TCAs were performed on plasma and urine samples. Relative recovery percentages (RR%) ranged from 90.3 % to 117.6 %. |
| format | Article |
| id | doaj-art-8effd04e32f74512bcd4e7d9c954b4a0 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
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| series | Advances in Sample Preparation |
| spelling | doaj-art-8effd04e32f74512bcd4e7d9c954b4a02024-12-25T04:21:41ZengElsevierAdvances in Sample Preparation2772-58202025-02-0113100145Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluidsSaeed Pourhosseini0Yadollah Yamini1Seyedeh Sara Nasrollahi2Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, IranCorresponding author.; Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, IranDepartment of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, IranChitosan intercalated montmorillonite and polyvinyl alcohol-citric acid (PVA/CA) nanofibers were electrospun onto stainless steel wires. The fibers were placed inside a stainless steel tube and utilized for fiber-in-tube solid phase microextraction (FIT-SPME) to detect trace levels of tricyclic antidepressant drugs (TCAs) in biological samples. The analytes were separated and quantified using gas chromatography coupled with mass spectrometric detection (GC–MS). The effects of various parameters on the extraction efficiency, including pH (9), extraction time (25 min), extraction flow rate (3 mL min-1), salt addition (0 % w/v), type of eluent (ethanol 99 %), eluent volume (300 µL), and desorption flow rate (70 µL min-1), were investigated and optimized. Under optimal conditions, the linear dynamic range for doxepin, and sertraline was found to be 0.08–750.0 ng mL-1; for amitriptyline, desipramine, and trimipramine, it was 10.0–750.0 ng mL-1; and for carbamazepine and maprotiline, it was 50.0–750.0 ng mL-1. The proposed method exhibited low limits of detections in the range of 0.02–15.2 ng mL-1. Additionally, coefficients of determination (R²) exceeding 0.9911 were achieved, indicating excellent linearity for the calibration curves. To assess the feasibility of the FIT-SPME-GC–MS method, extraction and analysis of TCAs were performed on plasma and urine samples. Relative recovery percentages (RR%) ranged from 90.3 % to 117.6 %.http://www.sciencedirect.com/science/article/pii/S2772582024000421NanofibersBio-nanocompositesUrine analysisPlasma analysisElectrospun fibersTricyclic antidepressants |
| spellingShingle | Saeed Pourhosseini Yadollah Yamini Seyedeh Sara Nasrollahi Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids Advances in Sample Preparation Nanofibers Bio-nanocomposites Urine analysis Plasma analysis Electrospun fibers Tricyclic antidepressants |
| title | Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids |
| title_full | Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids |
| title_fullStr | Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids |
| title_full_unstemmed | Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids |
| title_short | Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids |
| title_sort | electrospun chitosan modified montmorillonite polyvinyl alcohol nanofibers as suitable coating for fiber in tube solid phase microextraction of trace amounts of tricyclic antidepressants in biological fluids |
| topic | Nanofibers Bio-nanocomposites Urine analysis Plasma analysis Electrospun fibers Tricyclic antidepressants |
| url | http://www.sciencedirect.com/science/article/pii/S2772582024000421 |
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