Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systems
Background and Objectives: The incidence of multidrug-resistant, Gram-negative organisms, isolated as the etiological agents of infections is ascending. The advent of novel antibiotics poses significant challenges, necessitating the optimization and utilization of extant antimicrobial agents. Cefop...
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| Format: | Article |
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4891 |
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| author | Uksim Qadri Sofiya Zaffar Saleem Javaid Wani Shugufta Roohi Munazah Aman Sabah Bhat Umaya Majid |
| author_facet | Uksim Qadri Sofiya Zaffar Saleem Javaid Wani Shugufta Roohi Munazah Aman Sabah Bhat Umaya Majid |
| author_sort | Uksim Qadri |
| collection | DOAJ |
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Background and Objectives: The incidence of multidrug-resistant, Gram-negative organisms, isolated as the etiological agents of infections is ascending. The advent of novel antibiotics poses significant challenges, necessitating the optimization and utilization of extant antimicrobial agents. Cefoperazone, a third-generation cephalosporin and β-lactam antimicrobial, when combined with sulbactam, an irreversible β-lactamase inhibitor, mitigates the vulnerability of cefoperazone to β-lactamase-producing organisms. Nonetheless, regional data on the susceptibility patterns for this pharmacological combination remains scarce. The primary objective of this investigation was to assess the efficacy of the cefoperazone-sulbactam combination against prevalent Gram-negative bacteria isolated from blood cultures.
Materials and Methods: A total of 700 Gram-negative isolates, comprising Escherichia coli, Klebsiella pneumoniae, Acinetobacter species, and Pseudomonas aeruginosa, were procured using the BacT/Alert 3D system. The identification and susceptibility testing for cefoperazone-sulbactam were performed using the VITEK Compact ID and AST system. Comparative analysis was conducted against other tested antibiotics.
Results: The study revealed that cefoperazone-sulbactam exhibited commendable in-vitro activity against Gram-negative pathogens isolated from blood, surpassed only by colistin and tigecycline.
Conclusion: Cefoperazone-sulbactam demonstrates robust activity against the most frequently encountered clinical pathogens, suggesting its potential as an efficacious therapeutic agent. The findings underscore the imperative for ongoing surveillance of resistance patterns and trends among commonly used antimicrobials.
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| format | Article |
| id | doaj-art-8e6eead95bae47d5967ec8ccf62c2494 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-8e6eead95bae47d5967ec8ccf62c24942025-01-06T08:38:49ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-12-0116610.18502/ijm.v16i6.17245Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systemsUksim Qadri0Sofiya Zaffar1Saleem Javaid Wani2Shugufta Roohi3Munazah Aman4Sabah Bhat5Umaya Majid6Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, IndiaDepartment of General Medicine, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, India Background and Objectives: The incidence of multidrug-resistant, Gram-negative organisms, isolated as the etiological agents of infections is ascending. The advent of novel antibiotics poses significant challenges, necessitating the optimization and utilization of extant antimicrobial agents. Cefoperazone, a third-generation cephalosporin and β-lactam antimicrobial, when combined with sulbactam, an irreversible β-lactamase inhibitor, mitigates the vulnerability of cefoperazone to β-lactamase-producing organisms. Nonetheless, regional data on the susceptibility patterns for this pharmacological combination remains scarce. The primary objective of this investigation was to assess the efficacy of the cefoperazone-sulbactam combination against prevalent Gram-negative bacteria isolated from blood cultures. Materials and Methods: A total of 700 Gram-negative isolates, comprising Escherichia coli, Klebsiella pneumoniae, Acinetobacter species, and Pseudomonas aeruginosa, were procured using the BacT/Alert 3D system. The identification and susceptibility testing for cefoperazone-sulbactam were performed using the VITEK Compact ID and AST system. Comparative analysis was conducted against other tested antibiotics. Results: The study revealed that cefoperazone-sulbactam exhibited commendable in-vitro activity against Gram-negative pathogens isolated from blood, surpassed only by colistin and tigecycline. Conclusion: Cefoperazone-sulbactam demonstrates robust activity against the most frequently encountered clinical pathogens, suggesting its potential as an efficacious therapeutic agent. The findings underscore the imperative for ongoing surveillance of resistance patterns and trends among commonly used antimicrobials. https://ijm.tums.ac.ir/index.php/ijm/article/view/4891Antimicrobial resistance;Cefoperazone/sulbactam;Gram negative isolates |
| spellingShingle | Uksim Qadri Sofiya Zaffar Saleem Javaid Wani Shugufta Roohi Munazah Aman Sabah Bhat Umaya Majid Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systems Iranian Journal of Microbiology Antimicrobial resistance; Cefoperazone/sulbactam; Gram negative isolates |
| title | Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systems |
| title_full | Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systems |
| title_fullStr | Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systems |
| title_full_unstemmed | Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systems |
| title_short | Evaluating the in vitro activity of cefoperazone-sulbactam against Gram negative pathogens in blood stream infections using automated systems |
| title_sort | evaluating the in vitro activity of cefoperazone sulbactam against gram negative pathogens in blood stream infections using automated systems |
| topic | Antimicrobial resistance; Cefoperazone/sulbactam; Gram negative isolates |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4891 |
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