Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system
CRISPR/Cas9-based multiplex genome editing via electroporation is relatively efficient; however, lipofection is versatile because of its ease of use and low cost. Here, we aimed to determine the efficiency of lipofection in CRISPR/Cas9-based multiplex genome editing using growth hormone receptor (GH...
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| Main Authors: | Qingyi LIN, Koki TAKEBAYASHI, Nanaka TORIGOE, Bin LIU, Zhao NAMULA, Maki HIRATA, Fuminori TANIHARA, Megumi NAGAHARA, Takeshige OTOI |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Society for Reproduction and Development
2024-08-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/70/6/70_2024-054/_pdf/-char/en |
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