Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system

Genome editing of porcine zygotes via lipofection of two guide RNAs using a CRISPR/Cas9 system

CRISPR/Cas9-based multiplex genome editing via electroporation is relatively efficient; however, lipofection is versatile because of its ease of use and low cost. Here, we aimed to determine the efficiency of lipofection in CRISPR/Cas9-based multiplex genome editing using growth hormone receptor (GH...

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Bibliographic Details
Main Authors: Qingyi LIN, Koki TAKEBAYASHI, Nanaka TORIGOE, Bin LIU, Zhao NAMULA, Maki HIRATA, Fuminori TANIHARA, Megumi NAGAHARA, Takeshige OTOI
Format: Article
Language:English
Published: The Society for Reproduction and Development 2024-08-01
Series:The Journal of Reproduction and Development
Subjects:
crispr/cas9 system
growth hormone receptor (ghr)
glycoprotein alpha-galactosyltransferase 1 (ggta1)
lipofection
porcine zygote
Online Access:https://www.jstage.jst.go.jp/article/jrd/70/6/70_2024-054/_pdf/-char/en
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https://www.jstage.jst.go.jp/article/jrd/70/6/70_2024-054/_pdf/-char/en

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