Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system

CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications. CRISPR-Cas systems can be categorized into six different types ba...

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Main Authors: Hui Cheng, Haoyue Deng, Dongdao Ma, Mengyuan Gao, Zhihan Zhou, Heng Li, Shejuan Liu, Tieshan Teng
Format: Article
Language:English
Published: Elsevier 2024-10-01
Series:Heliyon
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024155697
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author Hui Cheng
Haoyue Deng
Dongdao Ma
Mengyuan Gao
Zhihan Zhou
Heng Li
Shejuan Liu
Tieshan Teng
author_facet Hui Cheng
Haoyue Deng
Dongdao Ma
Mengyuan Gao
Zhihan Zhou
Heng Li
Shejuan Liu
Tieshan Teng
author_sort Hui Cheng
collection DOAJ
description CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications. CRISPR-Cas systems can be categorized into six different types based on their composition and mechanism, and there are also natural regulatory biomolecules in bacteria and bacteriophages that can either enhance or inhibit the immune function of CRISPR-Cas. The CRISPR-Cas systems are currently being trialed as a new tool for gene therapy to treat various human diseases, including cancers and genetic diseases, offering significant therapeutic potential. This paper comprehensively summarizes various aspects of the CRISPR-Cas system, encompassing its diversity, regulatory mechanisms, its clinical applications and the obstacles encountered.
format Article
id doaj-art-8dfe70d9f09540d9b0d07040afccc4d4
institution Kabale University
issn 2405-8440
language English
publishDate 2024-10-01
publisher Elsevier
record_format Article
series Heliyon
spelling doaj-art-8dfe70d9f09540d9b0d07040afccc4d42024-11-12T05:20:54ZengElsevierHeliyon2405-84402024-10-011020e39538Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas systemHui Cheng0Haoyue Deng1Dongdao Ma2Mengyuan Gao3Zhihan Zhou4Heng Li5Shejuan Liu6Tieshan Teng7School of Basic Medical Sciences, Henan University, Kaifeng, 475004, ChinaSchool of Basic Medical Sciences, Henan University, Kaifeng, 475004, ChinaSchool of Basic Medical Sciences, Henan University, Kaifeng, 475004, ChinaSchool of Basic Medical Sciences, Henan University, Kaifeng, 475004, ChinaSchool of Basic Medical Sciences, Henan University, Kaifeng, 475004, ChinaSchool of Medical Laboratory, Weifang Medical University, Weifang, 261053, Shandong, ChinaSchool of Basic Medical Sciences, Henan University, Kaifeng, 475004, China; Corresponding author.School of Basic Medical Sciences, Henan University, Kaifeng, 475004, China; Corresponding author.CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications. CRISPR-Cas systems can be categorized into six different types based on their composition and mechanism, and there are also natural regulatory biomolecules in bacteria and bacteriophages that can either enhance or inhibit the immune function of CRISPR-Cas. The CRISPR-Cas systems are currently being trialed as a new tool for gene therapy to treat various human diseases, including cancers and genetic diseases, offering significant therapeutic potential. This paper comprehensively summarizes various aspects of the CRISPR-Cas system, encompassing its diversity, regulatory mechanisms, its clinical applications and the obstacles encountered.http://www.sciencedirect.com/science/article/pii/S2405844024155697CRISPR-CasGenome editingRegulatory mechanismClinical applications
spellingShingle Hui Cheng
Haoyue Deng
Dongdao Ma
Mengyuan Gao
Zhihan Zhou
Heng Li
Shejuan Liu
Tieshan Teng
Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system
Heliyon
CRISPR-Cas
Genome editing
Regulatory mechanism
Clinical applications
title Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system
title_full Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system
title_fullStr Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system
title_full_unstemmed Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system
title_short Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system
title_sort insight into the natural regulatory mechanisms and clinical applications of the crispr cas system
topic CRISPR-Cas
Genome editing
Regulatory mechanism
Clinical applications
url http://www.sciencedirect.com/science/article/pii/S2405844024155697
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