Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry
Abstract Background Equine melanocytic neoplasm (EMN) is a skin tumor commonly observed in grey horses. Limited research has yet to investigate proteomic profiles of EMN, particularly in the early stages and their expression patterns. This study, therefore, aimed to identify signature proteins from...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-08-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-025-04956-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849333194594189312 |
|---|---|
| author | Parichart Tesena Ruethaiwan Vinijkumthorn Amornthep Kingkaw Petchpailin Yanyongsirikarn Khajornpol Phasuk Sekkarin Ploypetch Narumon Phaonakrop Sittiruk Roytrakul Wanwipa Vongsangnak Nawarus Prapaiwan |
| author_facet | Parichart Tesena Ruethaiwan Vinijkumthorn Amornthep Kingkaw Petchpailin Yanyongsirikarn Khajornpol Phasuk Sekkarin Ploypetch Narumon Phaonakrop Sittiruk Roytrakul Wanwipa Vongsangnak Nawarus Prapaiwan |
| author_sort | Parichart Tesena |
| collection | DOAJ |
| description | Abstract Background Equine melanocytic neoplasm (EMN) is a skin tumor commonly observed in grey horses. Limited research has yet to investigate proteomic profiles of EMN, particularly in the early stages and their expression patterns. This study, therefore, aimed to identify signature proteins from tissue biopsies to distinguish early EMN, severe EMN, and normal groups. Results Using proteomic analysis of 19 tissue samples (normal: n = 6, early EMN: n = 7, severe EMN: n = 6) through LC–MS/MS, 12,310 proteins were identified. Differentially expressed proteins (DEPs) and functional interaction analysis revealed significant overexpression of Wnt signature proteins, e.g., canonical (Wnt2B) and non-canonical (Wnt5B) Wnt signaling in early EMN stages. Immunohistochemical staining (IHC) towards immunolocalizing Wnt signature protein, particularly the Wnt2B functional signal, further verified its higher expression in early EMN compared to other groups. Conclusions These findings suggest that the Wnt pathway and functional insight are key mediators in signal transduction during early EMN, offering potential markers for initial stage detection. This study enhances the understanding of EMN mechanisms and the role of Wnt proteins, with implications for developing future diagnostic and therapeutic strategies. |
| format | Article |
| id | doaj-art-8dfcf8f7c13a41bab0f36bce53f62419 |
| institution | Kabale University |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-8dfcf8f7c13a41bab0f36bce53f624192025-08-20T03:45:57ZengBMCBMC Veterinary Research1746-61482025-08-0121111810.1186/s12917-025-04956-wProbing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistryParichart Tesena0Ruethaiwan Vinijkumthorn1Amornthep Kingkaw2Petchpailin Yanyongsirikarn3Khajornpol Phasuk4Sekkarin Ploypetch5Narumon Phaonakrop6Sittiruk Roytrakul7Wanwipa Vongsangnak8Nawarus Prapaiwan9Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol UniversityDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol UniversityFaculty of Science, Interdisciplinary Graduate Program in Bioscience, Kasetsart UniversityFaculty of Veterinary Science, Equine Clinic, Prasuarthon Small Animal Hospital, Mahidol UniversityVeterinary Medicine Disposal and Animal Husbandry, Sub-Division Patrol Special Operation Division, Mounted Police Sub-DivisionDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol UniversityFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyDepartment of Zoology, Faculty of Science, Kasetsart UniversityDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol UniversityAbstract Background Equine melanocytic neoplasm (EMN) is a skin tumor commonly observed in grey horses. Limited research has yet to investigate proteomic profiles of EMN, particularly in the early stages and their expression patterns. This study, therefore, aimed to identify signature proteins from tissue biopsies to distinguish early EMN, severe EMN, and normal groups. Results Using proteomic analysis of 19 tissue samples (normal: n = 6, early EMN: n = 7, severe EMN: n = 6) through LC–MS/MS, 12,310 proteins were identified. Differentially expressed proteins (DEPs) and functional interaction analysis revealed significant overexpression of Wnt signature proteins, e.g., canonical (Wnt2B) and non-canonical (Wnt5B) Wnt signaling in early EMN stages. Immunohistochemical staining (IHC) towards immunolocalizing Wnt signature protein, particularly the Wnt2B functional signal, further verified its higher expression in early EMN compared to other groups. Conclusions These findings suggest that the Wnt pathway and functional insight are key mediators in signal transduction during early EMN, offering potential markers for initial stage detection. This study enhances the understanding of EMN mechanisms and the role of Wnt proteins, with implications for developing future diagnostic and therapeutic strategies.https://doi.org/10.1186/s12917-025-04956-wEquine melanocytic neoplasmGrey horsesImmunohistochemistryProteomicsWnt pathway |
| spellingShingle | Parichart Tesena Ruethaiwan Vinijkumthorn Amornthep Kingkaw Petchpailin Yanyongsirikarn Khajornpol Phasuk Sekkarin Ploypetch Narumon Phaonakrop Sittiruk Roytrakul Wanwipa Vongsangnak Nawarus Prapaiwan Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry BMC Veterinary Research Equine melanocytic neoplasm Grey horses Immunohistochemistry Proteomics Wnt pathway |
| title | Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry |
| title_full | Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry |
| title_fullStr | Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry |
| title_full_unstemmed | Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry |
| title_short | Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry |
| title_sort | probing wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry |
| topic | Equine melanocytic neoplasm Grey horses Immunohistochemistry Proteomics Wnt pathway |
| url | https://doi.org/10.1186/s12917-025-04956-w |
| work_keys_str_mv | AT paricharttesena probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT ruethaiwanvinijkumthorn probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT amornthepkingkaw probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT petchpailinyanyongsirikarn probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT khajornpolphasuk probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT sekkarinploypetch probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT narumonphaonakrop probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT sittirukroytrakul probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT wanwipavongsangnak probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry AT nawarusprapaiwan probingwntpathwayandfunctionalsignalinequinemelanocyticneoplasmsthroughquantitativeproteomicsandimmunohistochemistry |