Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes
Camels (<i>Camelus dromedarius</i>) are seasonal short-day breeders, regulated by photoperiod and melatonin secretion. However, no studies have explored melatonin levels in camel seminal plasma or their relationship with testosterone, age, or climatic factors, nor is it known whether mel...
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2025-01-01
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| author | Lamia Doghbri Melissa Carvajal-Serna Moufida Atigui Adriana Casao Victoria Peña-Delgado Mabrouk-Mouldi Seddik Mohamed Dbara Rosaura Pérez-Pé Mohamed Hammadi |
| author_facet | Lamia Doghbri Melissa Carvajal-Serna Moufida Atigui Adriana Casao Victoria Peña-Delgado Mabrouk-Mouldi Seddik Mohamed Dbara Rosaura Pérez-Pé Mohamed Hammadi |
| author_sort | Lamia Doghbri |
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| description | Camels (<i>Camelus dromedarius</i>) are seasonal short-day breeders, regulated by photoperiod and melatonin secretion. However, no studies have explored melatonin levels in camel seminal plasma or their relationship with testosterone, age, or climatic factors, nor is it known whether melatonin receptors exist in camel spermatozoa to respond to seminal melatonin. This study aimed to analyze melatonin levels in camel seminal plasma and its specific receptors in spermatozoa. Semen samples were obtained from November to March (breeding season). Testosterone and melatonin levels were measured in seminal plasma by ELISA. Melatonin receptors were localized in spermatozoa using immunofluorescence, and their presence was confirmed by Western Blot. Melatonin levels were higher from November to January and decreased in February and March. No correlation between testosterone and melatonin levels was found, but both hormones were negatively correlated with daylength (<i>p</i> = 0.0089 and <i>p</i> = 0.0688, respectively). Testosterone, but not melatonin, levels were affected by age. Two melatonin receptors (MT1, MT2) were detected on camel spermatozoa, with several immunotypes labeled mainly in the tail and post-acrosome region, but also in the acrosome and neck. Western Blot analysis confirmed the presence of these receptors, showing a 39 kDa band for MT1 and a 36 kDa band for MT2. Understanding melatonin’s effects on sperm could help ejaculates’ processing procedures, semen handling, and infertility issues in camels. |
| format | Article |
| id | doaj-art-8db41d93fb91465f9d8331754fc3e4a2 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-8db41d93fb91465f9d8331754fc3e4a22025-01-10T13:14:00ZengMDPI AGAnimals2076-26152025-01-011518310.3390/ani15010083Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm MembranesLamia Doghbri0Melissa Carvajal-Serna1Moufida Atigui2Adriana Casao3Victoria Peña-Delgado4Mabrouk-Mouldi Seddik5Mohamed Dbara6Rosaura Pérez-Pé7Mohamed Hammadi8Livestock and Wildlife Laboratory (LR16IRA04), Arid Lands Institute, University of Gabès, Médenine 4119, TunisiaGrupo BIOFITER, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, SpainLivestock and Wildlife Laboratory (LR16IRA04), Arid Lands Institute, University of Gabès, Médenine 4119, TunisiaGrupo BIOFITER, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, SpainGrupo BIOFITER, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, SpainLivestock and Wildlife Laboratory (LR16IRA04), Arid Lands Institute, University of Gabès, Médenine 4119, TunisiaLivestock and Wildlife Laboratory (LR16IRA04), Arid Lands Institute, University of Gabès, Médenine 4119, TunisiaGrupo BIOFITER, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, SpainLivestock and Wildlife Laboratory (LR16IRA04), Arid Lands Institute, University of Gabès, Médenine 4119, TunisiaCamels (<i>Camelus dromedarius</i>) are seasonal short-day breeders, regulated by photoperiod and melatonin secretion. However, no studies have explored melatonin levels in camel seminal plasma or their relationship with testosterone, age, or climatic factors, nor is it known whether melatonin receptors exist in camel spermatozoa to respond to seminal melatonin. This study aimed to analyze melatonin levels in camel seminal plasma and its specific receptors in spermatozoa. Semen samples were obtained from November to March (breeding season). Testosterone and melatonin levels were measured in seminal plasma by ELISA. Melatonin receptors were localized in spermatozoa using immunofluorescence, and their presence was confirmed by Western Blot. Melatonin levels were higher from November to January and decreased in February and March. No correlation between testosterone and melatonin levels was found, but both hormones were negatively correlated with daylength (<i>p</i> = 0.0089 and <i>p</i> = 0.0688, respectively). Testosterone, but not melatonin, levels were affected by age. Two melatonin receptors (MT1, MT2) were detected on camel spermatozoa, with several immunotypes labeled mainly in the tail and post-acrosome region, but also in the acrosome and neck. Western Blot analysis confirmed the presence of these receptors, showing a 39 kDa band for MT1 and a 36 kDa band for MT2. Understanding melatonin’s effects on sperm could help ejaculates’ processing procedures, semen handling, and infertility issues in camels.https://www.mdpi.com/2076-2615/15/1/83camelspermatozoatestosteronehumiditymelatonin membrane receptors |
| spellingShingle | Lamia Doghbri Melissa Carvajal-Serna Moufida Atigui Adriana Casao Victoria Peña-Delgado Mabrouk-Mouldi Seddik Mohamed Dbara Rosaura Pérez-Pé Mohamed Hammadi Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes Animals camel spermatozoa testosterone humidity melatonin membrane receptors |
| title | Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes |
| title_full | Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes |
| title_fullStr | Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes |
| title_full_unstemmed | Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes |
| title_short | Melatonin in Male Dromedary Camel (<i>Camelus dromedarius</i>) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes |
| title_sort | melatonin in male dromedary camel i camelus dromedarius i seminal plasma and its specific mt1 and mt2 receptors on sperm membranes |
| topic | camel spermatozoa testosterone humidity melatonin membrane receptors |
| url | https://www.mdpi.com/2076-2615/15/1/83 |
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