Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective Profile
This work is based on research aiming to extract and identify flavonoids from jaborandi (<i>Pilocarpus microphyllus</i>) leaves and investigate their antioxidant and acute antinociceptive capacity. Characterization of the constituents of the ethyl acetate fraction (EtOAcF) obtained from...
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2024-11-01
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| author | Márcia Luana Gomes Perfeito Fábio de Oliveira Silva Ribeiro Joilson Ramos de Jesus Leiz Maria Costa Véras Alyne Rodrigues de Araújo Nobre Everton Moraes Lopes José Carlos Eloi de Queiroz Andreanne Gomes Vasconcelos Miguel Gomes Cardoso João Gonçalves Fernanda Regina de Castro Almeida Daniel Dias Rufino Arcanjo José Roberto de Souza de Almeida Leite |
| author_facet | Márcia Luana Gomes Perfeito Fábio de Oliveira Silva Ribeiro Joilson Ramos de Jesus Leiz Maria Costa Véras Alyne Rodrigues de Araújo Nobre Everton Moraes Lopes José Carlos Eloi de Queiroz Andreanne Gomes Vasconcelos Miguel Gomes Cardoso João Gonçalves Fernanda Regina de Castro Almeida Daniel Dias Rufino Arcanjo José Roberto de Souza de Almeida Leite |
| author_sort | Márcia Luana Gomes Perfeito |
| collection | DOAJ |
| description | This work is based on research aiming to extract and identify flavonoids from jaborandi (<i>Pilocarpus microphyllus</i>) leaves and investigate their antioxidant and acute antinociceptive capacity. Characterization of the constituents of the ethyl acetate fraction (EtOAcF) obtained from the methanolic extract (ME) was performed by UV-Vis spectrophotometry, infrared spectroscopy, high-performance liquid chromatography (HPLC), mass spectrometry (MS), and cyclic voltammetry, demonstrating the possible majority component of this fraction, the flavone chrysin. Its solubility properties in HPLC are very close to those of the flavonol quercetin, revealing the characteristic presence of this group. An MS spectrum of the fraction revealed a major protonated molecule of m/z 254.9 [M+H]<sup>+</sup>. The EtOAcF fraction showed three oxidation processes at 0.32 V, 0.54 V, and 0.73 V vs. Ag/AgCl. Three reduction processes at the respective potentials: 0.60 V, −0.03 V, and -0.24 V vs. Ag/AgCl, indicating potential antioxidant activity. At DPPH and ABTS antioxidant radical capture assay, The IC<sub>50</sub> obtained was 0.5 mg/mL and 0.81 mg/mL, respectively. In vivo test to determine the mechanical nociceptive threshold in the von Frey test, the dose of 100 mg/kg of the EtOAcF was able to cause inhibition of behavioral changes in neuropathy. The results obtained in this study demonstrate the biological potential of an EtOAcF derived from jaborandi leaves. |
| format | Article |
| id | doaj-art-8d65ea7588944a719fa4b02a6449657e |
| institution | Kabale University |
| issn | 2813-2998 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Drugs and Drug Candidates |
| spelling | doaj-art-8d65ea7588944a719fa4b02a6449657e2024-12-27T14:20:16ZengMDPI AGDrugs and Drug Candidates2813-29982024-11-013479681210.3390/ddc3040045Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective ProfileMárcia Luana Gomes Perfeito0Fábio de Oliveira Silva Ribeiro1Joilson Ramos de Jesus2Leiz Maria Costa Véras3Alyne Rodrigues de Araújo Nobre4Everton Moraes Lopes5José Carlos Eloi de Queiroz6Andreanne Gomes Vasconcelos7Miguel Gomes Cardoso8João Gonçalves9Fernanda Regina de Castro Almeida10Daniel Dias Rufino Arcanjo11José Roberto de Souza de Almeida Leite12Biodiversity and Biotechnology Research Center (BIOTEC), Parnaíba Delta Federal University (UFDPar), Parnaíba 64202-020, BrazilBiodiversity and Biotechnology Research Center (BIOTEC), Parnaíba Delta Federal University (UFDPar), Parnaíba 64202-020, BrazilBiodiversity and Biotechnology Research Center (BIOTEC), Parnaíba Delta Federal University (UFDPar), Parnaíba 64202-020, BrazilBiodiversity and Biotechnology Research Center (BIOTEC), Parnaíba Delta Federal University (UFDPar), Parnaíba 64202-020, BrazilBiodiversity and Biotechnology Research Center (BIOTEC), Parnaíba Delta Federal University (UFDPar), Parnaíba 64202-020, BrazilResearch Center in Medicinal Plants, Federal University of Piauí (UFPI), Teresina 64202-020, BrazilUnified Education Center of the Federal District (UDF), Brasilia 70390-045, DF, BrazilUnified Education Center of the Federal District (UDF), Brasilia 70390-045, DF, BrazilResearch Center in Morphology and Applied Immunology (NuPMIA), Area of Morphology, Faculty of Medicine (FM), University of Brasilia (UnB), Brasilia 70910-900, BraziliMed.ULisboa, Faculdade de Farmácia, Universidade de Lisboa, 1600-277 Lisboa, PortugalResearch Center in Medicinal Plants, Federal University of Piauí (UFPI), Teresina 64202-020, BrazilLAFMOL—Laboratory of Functional and Molecular Studies in Physiopharmacology, Department of Biophysics and Physiology, Federal University of Piauí, Teresina 64049-550, BrazilBiodiversity and Biotechnology Research Center (BIOTEC), Parnaíba Delta Federal University (UFDPar), Parnaíba 64202-020, BrazilThis work is based on research aiming to extract and identify flavonoids from jaborandi (<i>Pilocarpus microphyllus</i>) leaves and investigate their antioxidant and acute antinociceptive capacity. Characterization of the constituents of the ethyl acetate fraction (EtOAcF) obtained from the methanolic extract (ME) was performed by UV-Vis spectrophotometry, infrared spectroscopy, high-performance liquid chromatography (HPLC), mass spectrometry (MS), and cyclic voltammetry, demonstrating the possible majority component of this fraction, the flavone chrysin. Its solubility properties in HPLC are very close to those of the flavonol quercetin, revealing the characteristic presence of this group. An MS spectrum of the fraction revealed a major protonated molecule of m/z 254.9 [M+H]<sup>+</sup>. The EtOAcF fraction showed three oxidation processes at 0.32 V, 0.54 V, and 0.73 V vs. Ag/AgCl. Three reduction processes at the respective potentials: 0.60 V, −0.03 V, and -0.24 V vs. Ag/AgCl, indicating potential antioxidant activity. At DPPH and ABTS antioxidant radical capture assay, The IC<sub>50</sub> obtained was 0.5 mg/mL and 0.81 mg/mL, respectively. In vivo test to determine the mechanical nociceptive threshold in the von Frey test, the dose of 100 mg/kg of the EtOAcF was able to cause inhibition of behavioral changes in neuropathy. The results obtained in this study demonstrate the biological potential of an EtOAcF derived from jaborandi leaves.https://www.mdpi.com/2813-2998/3/4/45jaborandiflavonoidsantioxidant activityneuropathic pain |
| spellingShingle | Márcia Luana Gomes Perfeito Fábio de Oliveira Silva Ribeiro Joilson Ramos de Jesus Leiz Maria Costa Véras Alyne Rodrigues de Araújo Nobre Everton Moraes Lopes José Carlos Eloi de Queiroz Andreanne Gomes Vasconcelos Miguel Gomes Cardoso João Gonçalves Fernanda Regina de Castro Almeida Daniel Dias Rufino Arcanjo José Roberto de Souza de Almeida Leite Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective Profile Drugs and Drug Candidates jaborandi flavonoids antioxidant activity neuropathic pain |
| title | Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective Profile |
| title_full | Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective Profile |
| title_fullStr | Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective Profile |
| title_full_unstemmed | Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective Profile |
| title_short | Extraction and Identification of Flavonoids from the Leaves of <i>Pilocarpus microphyllus</i>: Focus on Antioxidant Activity and Neuroprotective Profile |
| title_sort | extraction and identification of flavonoids from the leaves of i pilocarpus microphyllus i focus on antioxidant activity and neuroprotective profile |
| topic | jaborandi flavonoids antioxidant activity neuropathic pain |
| url | https://www.mdpi.com/2813-2998/3/4/45 |
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