Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway
Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties. In this study, PC12, HepG2 cells, and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mecha...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250103 |
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| author | Xiaomei Du Kexin Wang Xiaoyan Sang Xiangxing Meng Jiao Xie Tianxin Wang Xiaozhi Liu Qun Huang Nan Zhang Hao Wang |
| author_facet | Xiaomei Du Kexin Wang Xiaoyan Sang Xiangxing Meng Jiao Xie Tianxin Wang Xiaozhi Liu Qun Huang Nan Zhang Hao Wang |
| author_sort | Xiaomei Du |
| collection | DOAJ |
| description | Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties. In this study, PC12, HepG2 cells, and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms. The results showed that naringin inhibited H2O2-induced decline in cell viability and decreased the content of reactive oxygen species in cells. Meanwhile, naringin prolonged the lifespan of f lies, enhanced the abilities of climbing and the resistance to stress, improved the activities of antioxidant enzymes, and decreased malondialdehyde content. Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells. Moreover, naringin down-regulated the mRNA expressions of inr, chico, pi3k, and akt-1, and up-regulated the mRNA expressions of dilp2, dilp3, dilp5, and foxo, thereby activating autophagy-related genes and increasing the number of lysosomes. Furthermore, the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling (IIS) pathway and activating the autophagy pathway, which was consistent with the result of network pharmacological predictions. |
| format | Article |
| id | doaj-art-8d4a48f55f414a1dbc20d801da619ee9 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-8d4a48f55f414a1dbc20d801da619ee92025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331231124510.26599/FSHW.2022.9250103Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathwayXiaomei Du0Kexin Wang1Xiaoyan Sang2Xiangxing Meng3Jiao Xie4Tianxin Wang5Xiaozhi Liu6Qun Huang7Nan Zhang8Hao Wang9State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaThe key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guizhou 550025, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaDepartment of neurosurgery, the Fifth Central Hospital of Tianjin, Tianjin 300450, ChinaThe key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guizhou 550025, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaNaringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties. In this study, PC12, HepG2 cells, and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms. The results showed that naringin inhibited H2O2-induced decline in cell viability and decreased the content of reactive oxygen species in cells. Meanwhile, naringin prolonged the lifespan of f lies, enhanced the abilities of climbing and the resistance to stress, improved the activities of antioxidant enzymes, and decreased malondialdehyde content. Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells. Moreover, naringin down-regulated the mRNA expressions of inr, chico, pi3k, and akt-1, and up-regulated the mRNA expressions of dilp2, dilp3, dilp5, and foxo, thereby activating autophagy-related genes and increasing the number of lysosomes. Furthermore, the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling (IIS) pathway and activating the autophagy pathway, which was consistent with the result of network pharmacological predictions.https://www.sciopen.com/article/10.26599/FSHW.2022.9250103drosophila melanogasterinsulin signaling (iis) pathwaynaringinpc12 cellhepg2 cell |
| spellingShingle | Xiaomei Du Kexin Wang Xiaoyan Sang Xiangxing Meng Jiao Xie Tianxin Wang Xiaozhi Liu Qun Huang Nan Zhang Hao Wang Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway Food Science and Human Wellness drosophila melanogaster insulin signaling (iis) pathway naringin pc12 cell hepg2 cell |
| title | Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway |
| title_full | Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway |
| title_fullStr | Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway |
| title_full_unstemmed | Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway |
| title_short | Naringin ameliorates H2O2-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway |
| title_sort | naringin ameliorates h2o2 induced oxidative damage in cells and prolongs the lifespan of female drosophila melanogaster via the insulin signaling pathway |
| topic | drosophila melanogaster insulin signaling (iis) pathway naringin pc12 cell hepg2 cell |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250103 |
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