Hypothetical Protein from Aspergillus niger contains Chromate Reductase Motifs
Chromate reductase is an enzyme that converts toxic and carcinogenic Cr6+ to non-carcinogenic and less soluble Cr3+. A hypothetical protein (ref.XP-001388504.1) from Aspergillus niger has been identified as chromate reductase using bioinformatics techniques. The techniques involved Clustal W alignm...
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| Format: | Article |
| Language: | English |
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Umaru Musa Yar'adua University, Katsina, Nigeria
2016-12-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/346 |
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| author | Sallau, A. B. Fiona Henriquez, Sani Ibrahim, Andrew Jonathan Nok H M Inuwa, Craig W. Roberts |
| author_facet | Sallau, A. B. Fiona Henriquez, Sani Ibrahim, Andrew Jonathan Nok H M Inuwa, Craig W. Roberts |
| author_sort | Sallau, A. B. |
| collection | DOAJ |
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Chromate reductase is an enzyme that converts toxic and carcinogenic Cr6+ to non-carcinogenic and less soluble Cr3+. A hypothetical protein (ref.XP-001388504.1) from Aspergillus niger has been identified as chromate reductase using bioinformatics techniques. The techniques involved Clustal W alignment and Basic Local Alignment Search Tool (BLAST). Using Clustal W alignment the motifs VTPEYN and LKNAID motifs were identified to be common among the different chromate reductase homologues aligned. The same motifs reappeared when the hypothetical protein was aligned with other chromate reductase homologues. The BLAST search was able to also identify VTPEYN and LKNAID motifs on the A. niger genome indicating the likelihood of the hypothetical protein being chromate reductase. The homologue was recovered as putative chromate reductase gene encoded by an open reading frame of 2227 bp nucleotide sequence. These findings clearly demonstrate the hypothetical protein is likely to be chromate reductase.
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| format | Article |
| id | doaj-art-8d46aa9950e049b392b83a6ca6e4fda6 |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-8d46aa9950e049b392b83a6ca6e4fda62024-11-28T11:10:04ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222016-12-011110.47430/ujmr.1611.016346Hypothetical Protein from Aspergillus niger contains Chromate Reductase MotifsSallau, A. B.0Fiona Henriquez,1Sani Ibrahim,2Andrew Jonathan Nok3H M Inuwa,4Craig W. Roberts5Department of Biochemistry Ahmadu Bello University, ZariaImmunology and Microbiology Department, University of West Scotland, Paisely UK Department of Biochemistry Ahmadu Bello University, ZariaDepartment of Biochemistry Ahmadu Bello University, ZariaDepartment of Biochemistry Ahmadu Bello University, ZariaImmunology and Microbiology Unit, Strathclyde Institute of Pharmacy and Biomedical Sciences, Glasgow, UK Chromate reductase is an enzyme that converts toxic and carcinogenic Cr6+ to non-carcinogenic and less soluble Cr3+. A hypothetical protein (ref.XP-001388504.1) from Aspergillus niger has been identified as chromate reductase using bioinformatics techniques. The techniques involved Clustal W alignment and Basic Local Alignment Search Tool (BLAST). Using Clustal W alignment the motifs VTPEYN and LKNAID motifs were identified to be common among the different chromate reductase homologues aligned. The same motifs reappeared when the hypothetical protein was aligned with other chromate reductase homologues. The BLAST search was able to also identify VTPEYN and LKNAID motifs on the A. niger genome indicating the likelihood of the hypothetical protein being chromate reductase. The homologue was recovered as putative chromate reductase gene encoded by an open reading frame of 2227 bp nucleotide sequence. These findings clearly demonstrate the hypothetical protein is likely to be chromate reductase. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/346Aspergillus nigergenemotifschromate reductase |
| spellingShingle | Sallau, A. B. Fiona Henriquez, Sani Ibrahim, Andrew Jonathan Nok H M Inuwa, Craig W. Roberts Hypothetical Protein from Aspergillus niger contains Chromate Reductase Motifs UMYU Journal of Microbiology Research Aspergillus niger gene motifs chromate reductase |
| title | Hypothetical Protein from Aspergillus niger contains Chromate Reductase Motifs |
| title_full | Hypothetical Protein from Aspergillus niger contains Chromate Reductase Motifs |
| title_fullStr | Hypothetical Protein from Aspergillus niger contains Chromate Reductase Motifs |
| title_full_unstemmed | Hypothetical Protein from Aspergillus niger contains Chromate Reductase Motifs |
| title_short | Hypothetical Protein from Aspergillus niger contains Chromate Reductase Motifs |
| title_sort | hypothetical protein from aspergillus niger contains chromate reductase motifs |
| topic | Aspergillus niger gene motifs chromate reductase |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/346 |
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