A size-invariant bud-duration timer enables robustness in yeast cell size control.
Cell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0209301 |
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| _version_ | 1846150970924335104 |
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| author | Corey A H Allard Franziska Decker Orion D Weiner Jared E Toettcher Brian R Graziano |
| author_facet | Corey A H Allard Franziska Decker Orion D Weiner Jared E Toettcher Brian R Graziano |
| author_sort | Corey A H Allard |
| collection | DOAJ |
| description | Cell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant 'timer' specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis. |
| format | Article |
| id | doaj-art-8d42d3a706ac48ea9bbf7fa026b54991 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-8d42d3a706ac48ea9bbf7fa026b549912024-11-28T05:31:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011312e020930110.1371/journal.pone.0209301A size-invariant bud-duration timer enables robustness in yeast cell size control.Corey A H AllardFranziska DeckerOrion D WeinerJared E ToettcherBrian R GrazianoCell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant 'timer' specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.https://doi.org/10.1371/journal.pone.0209301 |
| spellingShingle | Corey A H Allard Franziska Decker Orion D Weiner Jared E Toettcher Brian R Graziano A size-invariant bud-duration timer enables robustness in yeast cell size control. PLoS ONE |
| title | A size-invariant bud-duration timer enables robustness in yeast cell size control. |
| title_full | A size-invariant bud-duration timer enables robustness in yeast cell size control. |
| title_fullStr | A size-invariant bud-duration timer enables robustness in yeast cell size control. |
| title_full_unstemmed | A size-invariant bud-duration timer enables robustness in yeast cell size control. |
| title_short | A size-invariant bud-duration timer enables robustness in yeast cell size control. |
| title_sort | size invariant bud duration timer enables robustness in yeast cell size control |
| url | https://doi.org/10.1371/journal.pone.0209301 |
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