Concordance in diagnostic testing for respiratory pathogens of bighorn sheep
ABSTRACT Reliable diagnostic tests are essential for disease investigation and management. This is particularly true for diseases of free‐ranging wildlife where sampling is logistically difficult precluding retesting. Clinical assays for wildlife diseases frequently vary among laboratories because o...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-12-01
|
| Series: | Wildlife Society Bulletin |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/wsb.721 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846120080915562496 |
|---|---|
| author | Daniel P. Walsh E. Frances Cassirer Michael D. Bonds Daniel R. Brown William H. Edwards Glen C. Weiser Mark L. Drew Robert E. Briggs Karen A. Fox Michael W. Miller Sudarvili Shanthalingam Subramaniam Srikumaran Thomas E. Besser |
| author_facet | Daniel P. Walsh E. Frances Cassirer Michael D. Bonds Daniel R. Brown William H. Edwards Glen C. Weiser Mark L. Drew Robert E. Briggs Karen A. Fox Michael W. Miller Sudarvili Shanthalingam Subramaniam Srikumaran Thomas E. Besser |
| author_sort | Daniel P. Walsh |
| collection | DOAJ |
| description | ABSTRACT Reliable diagnostic tests are essential for disease investigation and management. This is particularly true for diseases of free‐ranging wildlife where sampling is logistically difficult precluding retesting. Clinical assays for wildlife diseases frequently vary among laboratories because of lack of appropriate standardized commercial kits. Results of diagnostic testing may also be called into question when investigators report different etiologies for disease outbreaks, despite similar clinical and pathologic findings. To evaluate reliability of diagnostic testing for respiratory pathogens of bighorn sheep (Ovis canadensis), we conducted a series of ring tests across 6 laboratories routinely involved in detection of Mycoplasma ovipneumoniae, Pasteurellaceae, lktA (the Pasteurellaceae gene encoding leukotoxin), and 3 reference laboratories. Consistency of results for replicate samples within laboratories was high (median agreement = 1.0). Agreement between laboratories was high for polymerase chain reaction (PCR) detection of M. ovipneumoniae and culture isolation of Mannheimia spp. and Bibersteinia trehalosi (median agreement = 0.89–0.95, Kappa = 0.65–0.74), and lower for PCR detection of Mannheimia spp. lktA (median agreement = 0.58, Kappa = 0.12). Most errors on defined status samples were false negatives, suggesting test sensitivity was a greater problem than specificity. However, tests for M. haemolytica and lktA yielded some false positive results. Despite differences in testing protocols, median agreement among laboratories and correct classification of controls for most agents was ≥0.80, meeting or exceeding the standard required by federal proficiency testing programs. This information is valuable for interpreting test results, laboratory quality assessments, and advancing diagnosis of respiratory disease in wild sheep. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. |
| format | Article |
| id | doaj-art-8cf8088ebfc14b0ab96fb3d91446c68c |
| institution | Kabale University |
| issn | 2328-5540 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Wildlife Society Bulletin |
| spelling | doaj-art-8cf8088ebfc14b0ab96fb3d91446c68c2024-12-16T13:16:30ZengWileyWildlife Society Bulletin2328-55402016-12-0140463464210.1002/wsb.721Concordance in diagnostic testing for respiratory pathogens of bighorn sheepDaniel P. Walsh0E. Frances Cassirer1Michael D. Bonds2Daniel R. Brown3William H. Edwards4Glen C. Weiser5Mark L. Drew6Robert E. Briggs7Karen A. Fox8Michael W. Miller9Sudarvili Shanthalingam10Subramaniam Srikumaran11Thomas E. Besser12U.S. Geological SurveyNational Wildlife Health Center6006 Schroeder RoadMadisonWI53711USAIdaho Department of Fish and Game3316 16th StreetLewistonID83501USAU.S. Geological SurveyNational Wildlife Health Center6006 Schroeder RoadMadisonWI53711USADepartment of Infectious Diseases and PathologyUniversity of Florida2015 SW 16th AvenueGainesvilleFL32611USAWyoming Game and Fish DepartmentWildlife Health Laboratory1174 Snowy Range RoadLaramieWY82070USACaine Veterinary Teaching CenterUniversity of Idaho1020 E Homedale RoadCaldwellID83607USAIdaho Department of Fish and GameWildlife Health Laboratory16569 S 10th AvenueCaldwellID83607USAU.S. Department of AgricultureNational Animal Disease Center1920 Dayton AvenueAmesIA50010USAColorado Parks and WildlifeWildlife Health Program4330 W Laporte AvenueFort CollinsCO80521USAColorado Parks and WildlifeWildlife Health Program4330 W Laporte AvenueFort CollinsCO80521USADepartment of Veterinary Microbiology and PathologyWashington State UniversityPullmanWA99164USADepartment of Veterinary Microbiology and PathologyWashington State UniversityPullmanWA99164USADepartment of Veterinary Microbiology and PathologyWashington State UniversityPullmanWA99164USAABSTRACT Reliable diagnostic tests are essential for disease investigation and management. This is particularly true for diseases of free‐ranging wildlife where sampling is logistically difficult precluding retesting. Clinical assays for wildlife diseases frequently vary among laboratories because of lack of appropriate standardized commercial kits. Results of diagnostic testing may also be called into question when investigators report different etiologies for disease outbreaks, despite similar clinical and pathologic findings. To evaluate reliability of diagnostic testing for respiratory pathogens of bighorn sheep (Ovis canadensis), we conducted a series of ring tests across 6 laboratories routinely involved in detection of Mycoplasma ovipneumoniae, Pasteurellaceae, lktA (the Pasteurellaceae gene encoding leukotoxin), and 3 reference laboratories. Consistency of results for replicate samples within laboratories was high (median agreement = 1.0). Agreement between laboratories was high for polymerase chain reaction (PCR) detection of M. ovipneumoniae and culture isolation of Mannheimia spp. and Bibersteinia trehalosi (median agreement = 0.89–0.95, Kappa = 0.65–0.74), and lower for PCR detection of Mannheimia spp. lktA (median agreement = 0.58, Kappa = 0.12). Most errors on defined status samples were false negatives, suggesting test sensitivity was a greater problem than specificity. However, tests for M. haemolytica and lktA yielded some false positive results. Despite differences in testing protocols, median agreement among laboratories and correct classification of controls for most agents was ≥0.80, meeting or exceeding the standard required by federal proficiency testing programs. This information is valuable for interpreting test results, laboratory quality assessments, and advancing diagnosis of respiratory disease in wild sheep. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.https://doi.org/10.1002/wsb.721Bayesian Kappa statisticclinical testleukotoxinMycoplasma ovipneumoniaePasteurellaceaepneumonia |
| spellingShingle | Daniel P. Walsh E. Frances Cassirer Michael D. Bonds Daniel R. Brown William H. Edwards Glen C. Weiser Mark L. Drew Robert E. Briggs Karen A. Fox Michael W. Miller Sudarvili Shanthalingam Subramaniam Srikumaran Thomas E. Besser Concordance in diagnostic testing for respiratory pathogens of bighorn sheep Wildlife Society Bulletin Bayesian Kappa statistic clinical test leukotoxin Mycoplasma ovipneumoniae Pasteurellaceae pneumonia |
| title | Concordance in diagnostic testing for respiratory pathogens of bighorn sheep |
| title_full | Concordance in diagnostic testing for respiratory pathogens of bighorn sheep |
| title_fullStr | Concordance in diagnostic testing for respiratory pathogens of bighorn sheep |
| title_full_unstemmed | Concordance in diagnostic testing for respiratory pathogens of bighorn sheep |
| title_short | Concordance in diagnostic testing for respiratory pathogens of bighorn sheep |
| title_sort | concordance in diagnostic testing for respiratory pathogens of bighorn sheep |
| topic | Bayesian Kappa statistic clinical test leukotoxin Mycoplasma ovipneumoniae Pasteurellaceae pneumonia |
| url | https://doi.org/10.1002/wsb.721 |
| work_keys_str_mv | AT danielpwalsh concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT efrancescassirer concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT michaeldbonds concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT danielrbrown concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT williamhedwards concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT glencweiser concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT markldrew concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT robertebriggs concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT karenafox concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT michaelwmiller concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT sudarvilishanthalingam concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT subramaniamsrikumaran concordanceindiagnostictestingforrespiratorypathogensofbighornsheep AT thomasebesser concordanceindiagnostictestingforrespiratorypathogensofbighornsheep |