TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infection
IntroductionTLR7 detects the presence of single-stranded RNA (ssRNA) viruses, including human T-lymphotropic virus 1 (HTLV-1), and triggers antiviral and inflammatory responses that are responsible for infection control. Genetic variations in the TLR7 gene may alter cytokine production and influence...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1462352/full |
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| author | Emmanuelle Giuliana Mendes Santana Fabiane dos Santos Ferreira Wandrey Roberto dos Santos Brito Felipe Teixeira Lopes Aline Cecy Rocha de Lima Gabriel dos Santos Pereira Neto Ednelza da Silva Graça Amoras Sandra Souza Lima Carlos Araujo da Costa Maísa Silva Souza Ricardo Ishak Izaura Maria Vieira Cayres-Vallinoto Antonio Carlos Rosário Vallinoto Maria Alice Freitas Queiroz |
| author_facet | Emmanuelle Giuliana Mendes Santana Fabiane dos Santos Ferreira Wandrey Roberto dos Santos Brito Felipe Teixeira Lopes Aline Cecy Rocha de Lima Gabriel dos Santos Pereira Neto Ednelza da Silva Graça Amoras Sandra Souza Lima Carlos Araujo da Costa Maísa Silva Souza Ricardo Ishak Izaura Maria Vieira Cayres-Vallinoto Antonio Carlos Rosário Vallinoto Maria Alice Freitas Queiroz |
| author_sort | Emmanuelle Giuliana Mendes Santana |
| collection | DOAJ |
| description | IntroductionTLR7 detects the presence of single-stranded RNA (ssRNA) viruses, including human T-lymphotropic virus 1 (HTLV-1), and triggers antiviral and inflammatory responses that are responsible for infection control. Genetic variations in the TLR7 gene may alter cytokine production and influence the course of HTLV-1 infection. In the present study, the associations of TLR7 gene polymorphisms with HTLV-1-related symptoms, receptor expression levels, IFN-α and TNF-α levels and the proviral load were investigated.MethodsBlood samples from 159 individuals with HTLV-1 infection (66 with inflammatory diseases and 93 asymptomatic individuals) and 159 controls were collected. The genotyping of polymorphisms, TLR7 gene expression analysis and the quantification of the proviral load were performed by real-time PCR, and cytokine measurement was performed by enzyme-linked immunosorbent assay (ELISA).ResultsCarriers of the polymorphic allele for TLR7 rs179008 (A/T) had lower levels of IFN-α, while carriers of the polymorphic allele for TLR7 rs3853839 (C/G) had higher levels of TLR7 and IFN-α expression. The polymorphisms were not associated with symptoms of diseases related to HTLV-1 infection. The combination of A/G alleles for the TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms was associated with increased IFN-α levels and a decreased proviral load.DiscussionAlthough the polymorphisms did not influence the presence of symptoms of diseases caused by HTLV-1, carriers of the wild-type alleles for TLR7 rs179008 (A/T) and the polymorphism for TLR7 rs3853839 (C/G) appears to have a stronger antiviral response and increased infection control. |
| format | Article |
| id | doaj-art-8cd004eae3a84be7a3ab07a02307b9f9 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-8cd004eae3a84be7a3ab07a02307b9f92024-11-22T05:10:20ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-11-011510.3389/fimmu.2024.14623521462352TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infectionEmmanuelle Giuliana Mendes Santana0Fabiane dos Santos Ferreira1Wandrey Roberto dos Santos Brito2Felipe Teixeira Lopes3Aline Cecy Rocha de Lima4Gabriel dos Santos Pereira Neto5Ednelza da Silva Graça Amoras6Sandra Souza Lima7Carlos Araujo da Costa8Maísa Silva Souza9Ricardo Ishak10Izaura Maria Vieira Cayres-Vallinoto11Antonio Carlos Rosário Vallinoto12Maria Alice Freitas Queiroz13Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Cellular and Molecular Biology, Tropical Medicine Center, Federal University of Pará, Belém, BrazilLaboratory of Cellular and Molecular Biology, Tropical Medicine Center, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Virology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilIntroductionTLR7 detects the presence of single-stranded RNA (ssRNA) viruses, including human T-lymphotropic virus 1 (HTLV-1), and triggers antiviral and inflammatory responses that are responsible for infection control. Genetic variations in the TLR7 gene may alter cytokine production and influence the course of HTLV-1 infection. In the present study, the associations of TLR7 gene polymorphisms with HTLV-1-related symptoms, receptor expression levels, IFN-α and TNF-α levels and the proviral load were investigated.MethodsBlood samples from 159 individuals with HTLV-1 infection (66 with inflammatory diseases and 93 asymptomatic individuals) and 159 controls were collected. The genotyping of polymorphisms, TLR7 gene expression analysis and the quantification of the proviral load were performed by real-time PCR, and cytokine measurement was performed by enzyme-linked immunosorbent assay (ELISA).ResultsCarriers of the polymorphic allele for TLR7 rs179008 (A/T) had lower levels of IFN-α, while carriers of the polymorphic allele for TLR7 rs3853839 (C/G) had higher levels of TLR7 and IFN-α expression. The polymorphisms were not associated with symptoms of diseases related to HTLV-1 infection. The combination of A/G alleles for the TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms was associated with increased IFN-α levels and a decreased proviral load.DiscussionAlthough the polymorphisms did not influence the presence of symptoms of diseases caused by HTLV-1, carriers of the wild-type alleles for TLR7 rs179008 (A/T) and the polymorphism for TLR7 rs3853839 (C/G) appears to have a stronger antiviral response and increased infection control.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1462352/fullHTLV-I infectiontoll-like receptor 7polymorphism geneticgene expression profileinterferon-alpha |
| spellingShingle | Emmanuelle Giuliana Mendes Santana Fabiane dos Santos Ferreira Wandrey Roberto dos Santos Brito Felipe Teixeira Lopes Aline Cecy Rocha de Lima Gabriel dos Santos Pereira Neto Ednelza da Silva Graça Amoras Sandra Souza Lima Carlos Araujo da Costa Maísa Silva Souza Ricardo Ishak Izaura Maria Vieira Cayres-Vallinoto Antonio Carlos Rosário Vallinoto Maria Alice Freitas Queiroz TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infection Frontiers in Immunology HTLV-I infection toll-like receptor 7 polymorphism genetic gene expression profile interferon-alpha |
| title | TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infection |
| title_full | TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infection |
| title_fullStr | TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infection |
| title_full_unstemmed | TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infection |
| title_short | TLR7 rs179008 (A/T) and TLR7 rs3853839 (C/G) polymorphisms are associated with variations in IFN-α levels in HTLV-1 infection |
| title_sort | tlr7 rs179008 a t and tlr7 rs3853839 c g polymorphisms are associated with variations in ifn α levels in htlv 1 infection |
| topic | HTLV-I infection toll-like receptor 7 polymorphism genetic gene expression profile interferon-alpha |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1462352/full |
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