Effects of Huoshanhuangya tea extract on lipid accumulation and inflamma-tory response in palmitic acid-induced cells
This study aimed to compare and analyze the major biochemical components and antioxidant activities of four different grades (special-grade 1, special-grade 2, grade 1 and grade 2) of Huoshanhuangya tea. Special-grade 1 Huoshanhuangya tea was chosen to study the effects of Huoshanhuangya tea extract...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2024-08-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.05.102 |
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| Summary: | This study aimed to compare and analyze the major biochemical components and antioxidant activities of four different grades (special-grade 1, special-grade 2, grade 1 and grade 2) of Huoshanhuangya tea. Special-grade 1 Huoshanhuangya tea was chosen to study the effects of Huoshanhuangya tea extract (HTE) on lipid accumulation and proinflammatory cytokines via palmitic acid-induced alpha mouse liver 12 (AML-12), BV-2, human kidney-2 (HK-2), and human gastric adenocarcinoma (AGS) cells. The results revealed that the special-grade 1 Huoshanhuangya tea had the lowest soluble sugar content, the highest caffeine content, catechins content and total free amino acid content, and the strongest antioxidant activity. After treatment with HTE at a certain concentration, the viability of palmitic acid-induced AML-12, BV-2, HK-2 and AGS cells significantly increased; the lipid accumulation induced by palmitic acid was inhibited; and the release levels of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) in palmitic acid-induced AML-12, BV-2 and HK-2 cells were significantly decreased. To further explore its mechanism of action, it was found that HTE down-regulated the expression of Toll-like receptor 4 (TLR4) and nuclear factor-κB RelA<italic/>(NF-κB p65) in the inflammatory signaling pathway of BV-2 cells, suggesting that HTE may relieve the palmitic acid-induced inflammatory damage of BV-2 cells by inhibiting the activation of the TLR4/NF-κB signaling pathway. |
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| ISSN: | 1008-9209 2097-5155 |