Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration
The nerve guide conduit is a favorable implant for repairing peripheral nerve injuries, which is vital for promoting nerve regeneration. Therefore, we integrated magnetic 3D Schwann cells (SC) into a 3D-printed nerve conduit made from polyurethane to establish an optimal microenvironment for neuron...
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| Format: | Article |
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Elsevier
2025-07-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127525006240 |
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| author | Yueh-Sheng Chen Jian-Jr Lee Yen-Hong Lin Yun-Jhen Lin Min-Hua Yu Cheng-Yu Chen Der-Yang Cho Ming-You Shie |
| author_facet | Yueh-Sheng Chen Jian-Jr Lee Yen-Hong Lin Yun-Jhen Lin Min-Hua Yu Cheng-Yu Chen Der-Yang Cho Ming-You Shie |
| author_sort | Yueh-Sheng Chen |
| collection | DOAJ |
| description | The nerve guide conduit is a favorable implant for repairing peripheral nerve injuries, which is vital for promoting nerve regeneration. Therefore, we integrated magnetic 3D Schwann cells (SC) into a 3D-printed nerve conduit made from polyurethane to establish an optimal microenvironment for neuron regeneration. The X-ray diffractometer and Fourier-transform infrared spectroscopy confirmed successful fabrication of a magnetic 3D cell block by incorporating iron oxide nanoparticles (IONP). The IONP-containing cell block, when exposed to static magnetic fields, exhibited enhanced cellular behavior, proliferation, and secretion of extracellular vesicles. Additionally, the nerve conduction was performed to evaluate the function of the regenerated nerves. These IONP-containing 3D cell block stimulated with the magnetic field exhibited higher nerve conduction velocity and reduced latency in the regenerated nerves. In vivo studies demonstrated an increased number and density of regenerated axons, along with more consistent and thicker myelin sheaths. The IONP and magnetic fields work synergistically to enhance the differentiation and proliferation of SC. This showcases their positive effect on promoting the regeneration of peripheral nerves after injuries. |
| format | Article |
| id | doaj-art-8c5ff79c3a9c48d1adcc1be8e01c5fb6 |
| institution | DOAJ |
| issn | 0264-1275 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj-art-8c5ff79c3a9c48d1adcc1be8e01c5fb62025-08-20T03:22:00ZengElsevierMaterials & Design0264-12752025-07-0125511420410.1016/j.matdes.2025.114204Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regenerationYueh-Sheng Chen0Jian-Jr Lee1Yen-Hong Lin2Yun-Jhen Lin3Min-Hua Yu4Cheng-Yu Chen5Der-Yang Cho6Ming-You Shie7School of Chinese Medicine, China Medical University, Taichung 406040, TaiwanSchool of Medicine, China Medical University, Taichung 406040, Taiwan; Department of Plastic and Reconstructive Surgery, China Medical University Hospital, Taichung 404332, Taiwan; Xenotransplantation Translational Research Center, China Medical University Hospital, Taichung 404332, TaiwanDepartment of Biomedical Engineering, China Medical University, Taichung 406040, Taiwan; Research & Development Center for x-Dimensional Extracellular Vesicles, China Medical University Hospital, Taichung 404332, TaiwanSchool of Medicine, China Medical University, Taichung 406040, TaiwanXenotransplantation Translational Research Center, China Medical University Hospital, Taichung 404332, Taiwan; Institute of Translational Medicine and New Drug Development, China Medical University, Taichung 406040, TaiwanResearch & Development Center for x-Dimensional Extracellular Vesicles, China Medical University Hospital, Taichung 404332, TaiwanXenotransplantation Translational Research Center, China Medical University Hospital, Taichung 404332, Taiwan; Research & Development Center for x-Dimensional Extracellular Vesicles, China Medical University Hospital, Taichung 404332, Taiwan; Department of Neurosurgery, China Medical University Hospital, Taichung 404332, Taiwan; Translational Cell Therapy Center, China Medical University Hospital, Taichung 404332, Taiwan; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan; Corresponding author at: Graduate Institute of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan.Xenotransplantation Translational Research Center, China Medical University Hospital, Taichung 404332, Taiwan; Department of Biomedical Engineering, China Medical University, Taichung 406040, Taiwan; Research & Development Center for x-Dimensional Extracellular Vesicles, China Medical University Hospital, Taichung 404332, Taiwan; Department of Bioinformatics and Medical Engineering, Asia University, Taichung 413305, Taiwan; Corresponding author at: Department of Biomedical Engineering, China Medical University, Taichung 406040, Taiwan.The nerve guide conduit is a favorable implant for repairing peripheral nerve injuries, which is vital for promoting nerve regeneration. Therefore, we integrated magnetic 3D Schwann cells (SC) into a 3D-printed nerve conduit made from polyurethane to establish an optimal microenvironment for neuron regeneration. The X-ray diffractometer and Fourier-transform infrared spectroscopy confirmed successful fabrication of a magnetic 3D cell block by incorporating iron oxide nanoparticles (IONP). The IONP-containing cell block, when exposed to static magnetic fields, exhibited enhanced cellular behavior, proliferation, and secretion of extracellular vesicles. Additionally, the nerve conduction was performed to evaluate the function of the regenerated nerves. These IONP-containing 3D cell block stimulated with the magnetic field exhibited higher nerve conduction velocity and reduced latency in the regenerated nerves. In vivo studies demonstrated an increased number and density of regenerated axons, along with more consistent and thicker myelin sheaths. The IONP and magnetic fields work synergistically to enhance the differentiation and proliferation of SC. This showcases their positive effect on promoting the regeneration of peripheral nerves after injuries.http://www.sciencedirect.com/science/article/pii/S0264127525006240Schwann cellCell blockStatic magnetic fieldsIron oxideNerve guidance conduitsExtracellular vesicles |
| spellingShingle | Yueh-Sheng Chen Jian-Jr Lee Yen-Hong Lin Yun-Jhen Lin Min-Hua Yu Cheng-Yu Chen Der-Yang Cho Ming-You Shie Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration Materials & Design Schwann cell Cell block Static magnetic fields Iron oxide Nerve guidance conduits Extracellular vesicles |
| title | Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration |
| title_full | Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration |
| title_fullStr | Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration |
| title_full_unstemmed | Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration |
| title_short | Effects of magnetic fields-stimulated 3D-Schwann cell-secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration |
| title_sort | effects of magnetic fields stimulated 3d schwann cell secreted extracellular vesicles regulate differentiation of neural stem cells and nerve regeneration |
| topic | Schwann cell Cell block Static magnetic fields Iron oxide Nerve guidance conduits Extracellular vesicles |
| url | http://www.sciencedirect.com/science/article/pii/S0264127525006240 |
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