Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit
The study aimed to investigate the effects of thifluzamide (2.67 mg/L) on ‘Huangguan’ pear fruit rot caused by <i>Rhizoctonia solani</i> during storage, as well as the activities of polygalacturonase (PG), pectin methylesterase (PME), polygalacturonic acid trans-eliminase (PGTE), pectin...
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2024-11-01
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| author | Yushuo Wu Weiwei Yan Xiaonan Sun Xinnan Zhang Yonghong Ge Xiaohui Jia |
| author_facet | Yushuo Wu Weiwei Yan Xiaonan Sun Xinnan Zhang Yonghong Ge Xiaohui Jia |
| author_sort | Yushuo Wu |
| collection | DOAJ |
| description | The study aimed to investigate the effects of thifluzamide (2.67 mg/L) on ‘Huangguan’ pear fruit rot caused by <i>Rhizoctonia solani</i> during storage, as well as the activities of polygalacturonase (PG), pectin methylesterase (PME), polygalacturonic acid trans-eliminase (PGTE), pectin methyl trans-eliminase (PMTE), xylanase, and pectate lyase (PL) secreted by <i>R. solani</i>. The results showed that thifluzamide treatment significantly inhibited the activities of PG, PME, PGTE, PMTE, xylanase, and PL secreted by <i>R. solani</i> after 3 days in vitro culture, compared to the control. Thifluzamide also increased the activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), and 4-coumarate CoA ligase (4CL), and the contents of flavonoids and total phenolic compounds in pear fruit. Furthermore, thifluzamide increased the expression of <i>PcPAL</i>, <i>PcC4H</i>, <i>Pc4CL</i>, <i>Pcβ-1,3-GA</i>, <i>PcLCH</i>, <i>PcF3H</i>, and <i>PcDFR</i> involved in phenylpropanoid metabolism in pear fruit. In conclusion, thifluzamide treatment reduced the infection ability of <i>R. solani</i> by inhibiting the expression of the genes encoding cell wall-degrading enzymes in <i>R. solani</i>. At the same time, it inhibited the activities of cell wall-degrading enzymes induced resistance against <i>R. solani</i> infection in ‘Huangguan’ pears by promoting phenylpropane metabolism. |
| format | Article |
| id | doaj-art-8c5b3547f60f40aa9a095f248bb92ca8 |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-8c5b3547f60f40aa9a095f248bb92ca82024-11-26T18:16:48ZengMDPI AGPathogens2076-08172024-11-01131196310.3390/pathogens13110963Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear FruitYushuo Wu0Weiwei Yan1Xiaonan Sun2Xinnan Zhang3Yonghong Ge4Xiaohui Jia5Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaInstitute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaInstitute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaInstitute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaCollege of Food Science and Engineering, Bohai University, Jinzhou 121013, ChinaInstitute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaThe study aimed to investigate the effects of thifluzamide (2.67 mg/L) on ‘Huangguan’ pear fruit rot caused by <i>Rhizoctonia solani</i> during storage, as well as the activities of polygalacturonase (PG), pectin methylesterase (PME), polygalacturonic acid trans-eliminase (PGTE), pectin methyl trans-eliminase (PMTE), xylanase, and pectate lyase (PL) secreted by <i>R. solani</i>. The results showed that thifluzamide treatment significantly inhibited the activities of PG, PME, PGTE, PMTE, xylanase, and PL secreted by <i>R. solani</i> after 3 days in vitro culture, compared to the control. Thifluzamide also increased the activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), and 4-coumarate CoA ligase (4CL), and the contents of flavonoids and total phenolic compounds in pear fruit. Furthermore, thifluzamide increased the expression of <i>PcPAL</i>, <i>PcC4H</i>, <i>Pc4CL</i>, <i>Pcβ-1,3-GA</i>, <i>PcLCH</i>, <i>PcF3H</i>, and <i>PcDFR</i> involved in phenylpropanoid metabolism in pear fruit. In conclusion, thifluzamide treatment reduced the infection ability of <i>R. solani</i> by inhibiting the expression of the genes encoding cell wall-degrading enzymes in <i>R. solani</i>. At the same time, it inhibited the activities of cell wall-degrading enzymes induced resistance against <i>R. solani</i> infection in ‘Huangguan’ pears by promoting phenylpropane metabolism.https://www.mdpi.com/2076-0817/13/11/963<i>Rhizoctonia solani</i>thifluzamidepear fruitcell wall-degrading enzymephenylpropanoid pathway |
| spellingShingle | Yushuo Wu Weiwei Yan Xiaonan Sun Xinnan Zhang Yonghong Ge Xiaohui Jia Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit Pathogens <i>Rhizoctonia solani</i> thifluzamide pear fruit cell wall-degrading enzyme phenylpropanoid pathway |
| title | Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit |
| title_full | Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit |
| title_fullStr | Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit |
| title_full_unstemmed | Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit |
| title_short | Effects of Thifluzamide Treatment on the Production of Cell Wall Degrading Enzymes in <i>Rhizoctonia solani</i> and Phenylpropane Metabolism in Pear Fruit |
| title_sort | effects of thifluzamide treatment on the production of cell wall degrading enzymes in i rhizoctonia solani i and phenylpropane metabolism in pear fruit |
| topic | <i>Rhizoctonia solani</i> thifluzamide pear fruit cell wall-degrading enzyme phenylpropanoid pathway |
| url | https://www.mdpi.com/2076-0817/13/11/963 |
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