The bone Gla protein osteocalcin is expressed in cranial neural crest cells
Abstract Background Osteocalcin is a small protein abundant in the bone extracellular-matrix, that serves as a marker for mature osteoblasts. To become activated, osteocalcin undergoes a specific post-translational carboxylation. Osteocalcin is expressed at advanced stages of embryogenesis and after...
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BMC
2024-11-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-024-06990-7 |
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| author | Rotem Kalev-Altman Veatriki Fraggi-Rankis Efrat Monsonego-Ornan Dalit Sela-Donenfeld |
| author_facet | Rotem Kalev-Altman Veatriki Fraggi-Rankis Efrat Monsonego-Ornan Dalit Sela-Donenfeld |
| author_sort | Rotem Kalev-Altman |
| collection | DOAJ |
| description | Abstract Background Osteocalcin is a small protein abundant in the bone extracellular-matrix, that serves as a marker for mature osteoblasts. To become activated, osteocalcin undergoes a specific post-translational carboxylation. Osteocalcin is expressed at advanced stages of embryogenesis and after birth, when bone formation takes place. Neural crest cells (NCCs) are a unique cell population that evolves during early stages of development. While initially NCCs populate the dorsal neural-tube, later they undergo epithelial-to-mesenchymal-transition and migrate throughout the embryo in highly-regulated manner. NCCs give rise to multiple cell types including neurons and glia of the peripheral nervous system, chromaffin cells and skin melanocytes. Remarkably, in the head region, NCCs give rise to cartilage and bone. Finding: Here we report that osteocalcin is detected in cranial NCCs. Analysis of chick embryos at stages of cranial NCC migration revealed that osteocalcin mRNA and protein is expressed in pre-migratory and migratory NCCs in-vivo and ex-vivo. Addition of warfarin, an inhibitor of osteocalcin carboxylation, onto neural-tube explants, reduced the amount of NCC migration. These results provide the first evidence of osteocalcin presence in cranial NCCs, much before they give rise to craniofacial skeleton, and propose its possible involvement in the regulation of NCC migration. |
| format | Article |
| id | doaj-art-8c543f398a9444959e4ab7544822590a |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Research Notes |
| spelling | doaj-art-8c543f398a9444959e4ab7544822590a2024-11-10T12:05:14ZengBMCBMC Research Notes1756-05002024-11-011711910.1186/s13104-024-06990-7The bone Gla protein osteocalcin is expressed in cranial neural crest cellsRotem Kalev-Altman0Veatriki Fraggi-Rankis1Efrat Monsonego-Ornan2Dalit Sela-Donenfeld3Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew UniversityKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew UniversityThe Institute of Biochemistry and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew UniversityKoret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew UniversityAbstract Background Osteocalcin is a small protein abundant in the bone extracellular-matrix, that serves as a marker for mature osteoblasts. To become activated, osteocalcin undergoes a specific post-translational carboxylation. Osteocalcin is expressed at advanced stages of embryogenesis and after birth, when bone formation takes place. Neural crest cells (NCCs) are a unique cell population that evolves during early stages of development. While initially NCCs populate the dorsal neural-tube, later they undergo epithelial-to-mesenchymal-transition and migrate throughout the embryo in highly-regulated manner. NCCs give rise to multiple cell types including neurons and glia of the peripheral nervous system, chromaffin cells and skin melanocytes. Remarkably, in the head region, NCCs give rise to cartilage and bone. Finding: Here we report that osteocalcin is detected in cranial NCCs. Analysis of chick embryos at stages of cranial NCC migration revealed that osteocalcin mRNA and protein is expressed in pre-migratory and migratory NCCs in-vivo and ex-vivo. Addition of warfarin, an inhibitor of osteocalcin carboxylation, onto neural-tube explants, reduced the amount of NCC migration. These results provide the first evidence of osteocalcin presence in cranial NCCs, much before they give rise to craniofacial skeleton, and propose its possible involvement in the regulation of NCC migration.https://doi.org/10.1186/s13104-024-06990-7BGPOSTNeural tubeDelaminationCell motilityChondrogenesis |
| spellingShingle | Rotem Kalev-Altman Veatriki Fraggi-Rankis Efrat Monsonego-Ornan Dalit Sela-Donenfeld The bone Gla protein osteocalcin is expressed in cranial neural crest cells BMC Research Notes BGP OST Neural tube Delamination Cell motility Chondrogenesis |
| title | The bone Gla protein osteocalcin is expressed in cranial neural crest cells |
| title_full | The bone Gla protein osteocalcin is expressed in cranial neural crest cells |
| title_fullStr | The bone Gla protein osteocalcin is expressed in cranial neural crest cells |
| title_full_unstemmed | The bone Gla protein osteocalcin is expressed in cranial neural crest cells |
| title_short | The bone Gla protein osteocalcin is expressed in cranial neural crest cells |
| title_sort | bone gla protein osteocalcin is expressed in cranial neural crest cells |
| topic | BGP OST Neural tube Delamination Cell motility Chondrogenesis |
| url | https://doi.org/10.1186/s13104-024-06990-7 |
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