Protocol for identification of NEMF-mediated C-terminal extensions on mitochondrial nonstop proteins via customized MS/MS spectra database searching

Protocol for identification of NEMF-mediated C-terminal extensions on mitochondrial nonstop proteins via customized MS/MS spectra database searching

Summary: The ribosome-associated protein quality control (RQC) core factor nuclear export mediator factor (NEMF) appends C-terminal extended sequences (CESs) to ribosome-stalled nascent chains (NCs). Specific CESs compositions could be directly recognized by enzymes and facilitate NC degradation. Ye...

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Bibliographic Details
Main Authors: Leijie Chen, Jinyou Mo, Yuyong Tan, Liang Lv, Jia Liu
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Proteomics
Protein expression and purification
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005318
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