The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study
Background: Noninvasive perinatal testing is a new method of screening for aneuploidy called cell-free DNA (cfDNA). Fetal fraction (FF) plays a crucial role in assessing the reliability of aneuploidy detection through noninvasive perinatal testing. Objective: We aimed to investigate the associat...
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Shahid Sadoughi University of Medical Sciences
2025-01-01
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| Series: | International Journal of Reproductive BioMedicine |
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| Online Access: | https://knepublishing.com/index.php/ijrm/article/view/17824 |
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| author | Maryam Aryavand Maryam Nurzadeh Marjan Ghaemi Sina Eskandari Delfan Vajiheh Marsoosi |
| author_facet | Maryam Aryavand Maryam Nurzadeh Marjan Ghaemi Sina Eskandari Delfan Vajiheh Marsoosi |
| author_sort | Maryam Aryavand |
| collection | DOAJ |
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Background: Noninvasive perinatal testing is a new method of screening for aneuploidy called cell-free DNA (cfDNA). Fetal fraction (FF) plays a crucial role in assessing the reliability of aneuploidy detection through noninvasive perinatal testing.
Objective: We aimed to investigate the association between the amount of FF in cfDNA testing and adverse pregnancy outcomes.
Materials and Methods: This cohort study was conducted on 619 singleton pregnant women who were candidates for cfDNA testing and were referred to the perinatology clinics of Shariati hospital and Arash Women’s hospital, both affiliated with Tehran University of Medical Sciences, Tehran, Iran from March 2019 to June 2020. The FF was extracted from the cfDNA test results, and the participants were followed until delivery.
Results: A total of 619 singleton pregnant women with a mean ± SD age and FF of 34.4 ± 4.85 and 8.39 ± 3.95, respectively, participated in the study. A significant association between maternal age and FF was not found (p = 0.12). A lower FF was associated with a rise in the incidence of gestational diabetes mellitus (p = 0.02) and a higher FF was associated with a rise in the incidence of fetal growth restriction (p < 0.001). However, high or low FF was not associated with pre-eclampsia, premature rupture of membranes, birth weight, or delivery time. No significant association was found between FF and multiple of the median of pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin.
Conclusion: The amount of FF may be considered a predictor of certain adverse pregnancy outcomes. Therefore, maternity care should be performed more carefully for women with high or low FF.
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| format | Article |
| id | doaj-art-8c1f236c14ca4671a5934210fec59252 |
| institution | Kabale University |
| issn | 2476-4108 2476-3772 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj-art-8c1f236c14ca4671a5934210fec592522025-01-15T06:16:56ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722025-01-01221110.18502/ijrm.v22i11.17824The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort studyMaryam Aryavand0Maryam Nurzadeh1Marjan Ghaemi2Sina Eskandari Delfan3 Vajiheh Marsoosi4Department of Obstetrics and Gynecology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.Department of Obstetrics and Gynecology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran.Vali-E-Asr Reproductive Health Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran.Department of Obstetrics and Gynecology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. Background: Noninvasive perinatal testing is a new method of screening for aneuploidy called cell-free DNA (cfDNA). Fetal fraction (FF) plays a crucial role in assessing the reliability of aneuploidy detection through noninvasive perinatal testing. Objective: We aimed to investigate the association between the amount of FF in cfDNA testing and adverse pregnancy outcomes. Materials and Methods: This cohort study was conducted on 619 singleton pregnant women who were candidates for cfDNA testing and were referred to the perinatology clinics of Shariati hospital and Arash Women’s hospital, both affiliated with Tehran University of Medical Sciences, Tehran, Iran from March 2019 to June 2020. The FF was extracted from the cfDNA test results, and the participants were followed until delivery. Results: A total of 619 singleton pregnant women with a mean ± SD age and FF of 34.4 ± 4.85 and 8.39 ± 3.95, respectively, participated in the study. A significant association between maternal age and FF was not found (p = 0.12). A lower FF was associated with a rise in the incidence of gestational diabetes mellitus (p = 0.02) and a higher FF was associated with a rise in the incidence of fetal growth restriction (p < 0.001). However, high or low FF was not associated with pre-eclampsia, premature rupture of membranes, birth weight, or delivery time. No significant association was found between FF and multiple of the median of pregnancy-associated plasma protein-A and free β-human chorionic gonadotropin. Conclusion: The amount of FF may be considered a predictor of certain adverse pregnancy outcomes. Therefore, maternity care should be performed more carefully for women with high or low FF. https://knepublishing.com/index.php/ijrm/article/view/17824Noninvasive prenatal testing, Cell-free DNA, Pre-eclampsia. |
| spellingShingle | Maryam Aryavand Maryam Nurzadeh Marjan Ghaemi Sina Eskandari Delfan Vajiheh Marsoosi The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study International Journal of Reproductive BioMedicine Noninvasive prenatal testing, Cell-free DNA, Pre-eclampsia. |
| title | The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study |
| title_full | The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study |
| title_fullStr | The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study |
| title_full_unstemmed | The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study |
| title_short | The association between the amount of fetal fraction in cell-free DNA testing and adverse pregnancy outcomes: A cohort study |
| title_sort | association between the amount of fetal fraction in cell free dna testing and adverse pregnancy outcomes a cohort study |
| topic | Noninvasive prenatal testing, Cell-free DNA, Pre-eclampsia. |
| url | https://knepublishing.com/index.php/ijrm/article/view/17824 |
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