Evaluation of V3–V4 and FL-16S rRNA amplicon sequencing approach for microbiota community analysis of tracheostomy aspirates

ABSTRACT Respiratory infections pose a significant risk for people requiring prolonged mechanical ventilation, yet limited information exists regarding the complex microbiome dynamics of people with tracheostomies during chronic critical illness. Oxford Nanopore Technologies (ONT) long-read sequenci...

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Bibliographic Details
Main Authors: A. Gupta, V. S. Cooper, A. C. Zemke
Format: Article
Language:English
Published: American Society for Microbiology 2025-08-01
Series:mSphere
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/msphere.00388-25
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