Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors
In vivo imaging of aminopeptidase N (APN/CD13) expression is crucial for the early detection of cancer. This study attempted to show that APN/CD13 expression can be imaged and quantified with novel Cerenkov luminescence tomography (CLT). Na 131 I with various activities was placed at different depth...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2013-05-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2012.00030 |
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| author | Zhenhua Hu Weidong Yang Xiaowei Ma Wenhui Ma Xiaochao Qu Jimin Liang Jing Wang Jie Tian |
| author_facet | Zhenhua Hu Weidong Yang Xiaowei Ma Wenhui Ma Xiaochao Qu Jimin Liang Jing Wang Jie Tian |
| author_sort | Zhenhua Hu |
| collection | DOAJ |
| description | In vivo imaging of aminopeptidase N (APN/CD13) expression is crucial for the early detection of cancer. This study attempted to show that APN/CD13 expression can be imaged and quantified with novel Cerenkov luminescence tomography (CLT). Na 131 I with various activities was placed at different depths in a tissue-mimicking phantom, and various porcine tissues and luminescent images were acquired. The binding of 131 I-NGR with human fibrosarcoma HT1080 and human colon cancer HT-29 cells was detected with Cerenkov luminescence imaging (CLI). Nude mice bearing HT-1080 tumors were imaged after injection with 131 I-NGR using both planar and tomographic CLI methods. The penetration depth increased with ascending activity of Na 131 I. There was a robust linear correlation between the optical signal intensity and the HT1080 cell numbers ( r 2 = .9691), as well as the activity ( r 2 = .9860). The three-dimensional visualization CLT results clearly showed that 131 I-NGR uptake in tumor tissues represented a high expression of the APN/CD13 receptor. CLT also allowed quantifying 131 I-NGR uptake in tumor tissues showing an average activity of 0.1388 ± 4.6788E-6 MBq in tumor tissues. Our study indicated that 131 I-NGR combined with CLT allowed us to image and quantify tumor-associated APN/CD13 expression noninvasively. The promising CLT technique could be potentially used for sensitively evaluating tumor angiogenesis in vivo. |
| format | Article |
| id | doaj-art-8bf4a0d25f63459c9f30a5a6551ffc82 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2013-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-8bf4a0d25f63459c9f30a5a6551ffc822025-01-03T01:22:54ZengSAGE PublishingMolecular Imaging1536-01212013-05-011210.2310/7290.2012.0003010.2310_7290.2012.00030Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 TumorsZhenhua HuWeidong YangXiaowei MaWenhui MaXiaochao QuJimin LiangJing WangJie TianIn vivo imaging of aminopeptidase N (APN/CD13) expression is crucial for the early detection of cancer. This study attempted to show that APN/CD13 expression can be imaged and quantified with novel Cerenkov luminescence tomography (CLT). Na 131 I with various activities was placed at different depths in a tissue-mimicking phantom, and various porcine tissues and luminescent images were acquired. The binding of 131 I-NGR with human fibrosarcoma HT1080 and human colon cancer HT-29 cells was detected with Cerenkov luminescence imaging (CLI). Nude mice bearing HT-1080 tumors were imaged after injection with 131 I-NGR using both planar and tomographic CLI methods. The penetration depth increased with ascending activity of Na 131 I. There was a robust linear correlation between the optical signal intensity and the HT1080 cell numbers ( r 2 = .9691), as well as the activity ( r 2 = .9860). The three-dimensional visualization CLT results clearly showed that 131 I-NGR uptake in tumor tissues represented a high expression of the APN/CD13 receptor. CLT also allowed quantifying 131 I-NGR uptake in tumor tissues showing an average activity of 0.1388 ± 4.6788E-6 MBq in tumor tissues. Our study indicated that 131 I-NGR combined with CLT allowed us to image and quantify tumor-associated APN/CD13 expression noninvasively. The promising CLT technique could be potentially used for sensitively evaluating tumor angiogenesis in vivo.https://doi.org/10.2310/7290.2012.00030 |
| spellingShingle | Zhenhua Hu Weidong Yang Xiaowei Ma Wenhui Ma Xiaochao Qu Jimin Liang Jing Wang Jie Tian Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors Molecular Imaging |
| title | Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors |
| title_full | Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors |
| title_fullStr | Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors |
| title_full_unstemmed | Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors |
| title_short | Cerenkov Luminescence Tomography of Aminopeptidase N (APN/CD13) Expression in Mice Bearing HT1080 Tumors |
| title_sort | cerenkov luminescence tomography of aminopeptidase n apn cd13 expression in mice bearing ht1080 tumors |
| url | https://doi.org/10.2310/7290.2012.00030 |
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