Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy
The alkamide content and specific tissue localization in the cypselae of <i>Heliopsis longipes</i> were investigated using gas chromatography–electron ionization mass spectrometry (GC-EIMS) and multiphoton fluorescence microscopy (MPFM). GC-EIMS analysis identified two olefinic alkamides...
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MDPI AG
2024-11-01
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| Online Access: | https://www.mdpi.com/1420-3049/29/23/5651 |
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| author | Juan Vázquez-Martínez Jorge Molina-Torres |
| author_facet | Juan Vázquez-Martínez Jorge Molina-Torres |
| author_sort | Juan Vázquez-Martínez |
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| description | The alkamide content and specific tissue localization in the cypselae of <i>Heliopsis longipes</i> were investigated using gas chromatography–electron ionization mass spectrometry (GC-EIMS) and multiphoton fluorescence microscopy (MPFM). GC-EIMS analysis identified two olefinic alkamides: affinin (spilanthol) and <i>N</i>-2-methylbutyl-2<i>E</i>,6<i>Z</i>,8<i>E</i>-decatrienamide. Microscopic analysis revealed that alkamides are localized within the cotyledons, and specifically compartmentalized in lipid bodies, highlighting their spatial organization. The linear unmixing of fluorescence emission fingerprints showed that affinin exhibits autofluorescence at 693 nm, corresponding to the red spectral region. This emission is attributed to the conjugated double bonds in its acyl chain. This study is the first to report on the presence and precise localization of alkamides in the cypselae of <i>H. longipes.</i> |
| format | Article |
| id | doaj-art-8bca3683ec544d98a42eb7f84a3f9cc7 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-8bca3683ec544d98a42eb7f84a3f9cc72024-12-13T16:28:35ZengMDPI AGMolecules1420-30492024-11-012923565110.3390/molecules29235651Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton MicroscopyJuan Vázquez-Martínez0Jorge Molina-Torres1Departamento de Ingeniería Química, TecNM/ITS Irapuato, Silao-Irapuato Km 12.5 El Copal, Irapuato CP 36821, GTO, MexicoDepartamento de Biotecnología y Bioquímica, Cinvestav Irapuato, Km. 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato CP 36821, GTO, MexicoThe alkamide content and specific tissue localization in the cypselae of <i>Heliopsis longipes</i> were investigated using gas chromatography–electron ionization mass spectrometry (GC-EIMS) and multiphoton fluorescence microscopy (MPFM). GC-EIMS analysis identified two olefinic alkamides: affinin (spilanthol) and <i>N</i>-2-methylbutyl-2<i>E</i>,6<i>Z</i>,8<i>E</i>-decatrienamide. Microscopic analysis revealed that alkamides are localized within the cotyledons, and specifically compartmentalized in lipid bodies, highlighting their spatial organization. The linear unmixing of fluorescence emission fingerprints showed that affinin exhibits autofluorescence at 693 nm, corresponding to the red spectral region. This emission is attributed to the conjugated double bonds in its acyl chain. This study is the first to report on the presence and precise localization of alkamides in the cypselae of <i>H. longipes.</i>https://www.mdpi.com/1420-3049/29/23/5651affininalkamidesGC-EIMS<i>Heliopsis longipes</i> seedsmultiphoton microscopyspilanthol |
| spellingShingle | Juan Vázquez-Martínez Jorge Molina-Torres Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy Molecules affinin alkamides GC-EIMS <i>Heliopsis longipes</i> seeds multiphoton microscopy spilanthol |
| title | Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy |
| title_full | Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy |
| title_fullStr | Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy |
| title_full_unstemmed | Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy |
| title_short | Alkamide Content and Localization in <i>Heliopsis longipes</i> Cypselae, Obtained via Fluorescence and Double-Multiphoton Microscopy |
| title_sort | alkamide content and localization in i heliopsis longipes i cypselae obtained via fluorescence and double multiphoton microscopy |
| topic | affinin alkamides GC-EIMS <i>Heliopsis longipes</i> seeds multiphoton microscopy spilanthol |
| url | https://www.mdpi.com/1420-3049/29/23/5651 |
| work_keys_str_mv | AT juanvazquezmartinez alkamidecontentandlocalizationiniheliopsislongipesicypselaeobtainedviafluorescenceanddoublemultiphotonmicroscopy AT jorgemolinatorres alkamidecontentandlocalizationiniheliopsislongipesicypselaeobtainedviafluorescenceanddoublemultiphotonmicroscopy |